Nokuba umthengi omtsha okanye umthengi omdala, sikholelwa ekuthetheni ixesha elide kunye nobudlelwane obuthembekileyo kwi-8 yeminyaka yokuThumela iSampuli yeVirus yaseChina kunye ne-Np Swab kunye ne-Op Swab ye-Specimen Collation kunye noThutho, iNasopharyngeal (Np) kunye ne-Urethral Cell Collection Flocked Swabs, Ukuba yinkampani ekhulayo ekhulayo, kodwa sisenokungabi lelona qabane lethu lisebenzayo.
Nokuba umthengi omtsha okanye umthengi oneminyaka ubudala, sikholelwa ekuchazeni ixesha elide kunye nobudlelwane obuthembekileyoityhubhu yokufumanisa i-nucleic acid, sixhomekeke kwiinzuzo zethu ukwakha indlela yorhwebo oluyinzuzo efanayo kunye namaqabane ethu entsebenziswano.Ngenxa yoko, sifumene uthungelwano lwentengiso lwehlabathi olufikelela kuMbindi Mpuma, eTurkey, eMalaysia naseVietnam.

Iinkcukacha

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 ingagcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho).

Nokuba umthengi omtsha okanye umthengi omdala, sikholelwa ekuthetheni ixesha elide kunye nobudlelwane obuthembekileyo kwi-8 yeminyaka yokuThumela iSampuli yeVirus yaseChina kunye ne-Np Swab kunye ne-Op Swab ye-Specimen Collation kunye noThutho, iNasopharyngeal (Np) kunye ne-Urethral Cell Collection Flocked Swabs, Ukuba yinkampani ekhulayo ekhulayo, kodwa sisenokungabi lelona qabane lethu lisebenzayo.
Iminyaka eyi-8 yomthengisi ngaphandle kwe-China Amies Medium, i-Amies, sithembele kwiinzuzo zethu ukwakha indlela yorhwebo oluxhamlayo kunye namaqabane ethu entsebenziswano.Ngenxa yoko, sifumene uthungelwano lwentengiso lwehlabathi olufikelela kuMbindi Mpuma, eTurkey, eMalaysia naseVietnam.