Singabavelisi abanamava.Ukuphumelela uninzi kwiziqinisekiso ezibalulekileyo zentengiso yayo ngexabiso eliphantsi China Lab 64 Well Plate Magnetic Bead Nucleic Acid ExtractionI-DNA Rna Yokucoca Isolation Kitnge CE, Kunye nemizamo yethu, iimveliso zethu ziye zaphumelela ukuthembela kubathengi kwaye zithengiswa kakhulu apha nakwamanye amazwe.
Singabavelisi abanamava.Ukuphumelela uninzi kwiziqinisekiso ezibalulekileyo zemarike yayoChina Nucleic Acid Extraction Kit, I-DNA Rna Yokucoca Isolation Kit, Singathanda ukumema abathengi abasuka phesheya ukuba baxoxe ngeshishini nathi.Singabonisa abathengi bethu iimveliso ezikumgangatho ophezulu kunye nenkonzo ebalaseleyo.Siqinisekile ukuba siya kuba nobudlelwane obuhle bentsebenziswano kwaye senze ikamva eliqaqambileyo lawo omabini amaqela.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) .Singumenzi onamava.Ukuphumelela uninzi kwizatifiketi ezibalulekileyo kwimarike yayo ngexabiso eliphantsi China Lab 64 Well Plate Magnetic Bead Nucleic Acid Extraction DNA Rna Purification Isolation Kit nge CE, Kunye nemizamo yethu, iimveliso zethu ziye zaphumelela intembeko yabathengi kwaye ibithengiswa kakhulu apha nakwamanye amazwe.
Ixabiso eliphantsiChina Nucleic Acid Extraction Kit, I-DNA Rna Purification Isolation Kit, Singathanda ukumema abathengi abavela phesheya ukuba baxoxe ngeshishini nathi.Singabonisa abathengi bethu iimveliso ezikumgangatho ophezulu kunye nenkonzo ebalaseleyo.Siqinisekile ukuba siya kuba nobudlelwane obuhle bentsebenziswano kwaye senze ikamva eliqaqambileyo lawo omabini amaqela.