I-China i-Wholesale yaseTshayina Lonke ixesha loBulunga obuGqwesileyo obuSebenzayo bePCR uMenzi kunye noMboneleli |I-Foregene

IWholeliseli yaseTshayina Lonke Ixesha loMgangatho oPhakamileyo wePCR eSebenzayo

China Ihowuliseleli China Lonke Ixesha loMgangatho uPhakamileyo wePCR oFakelweyo Umfanekiso

Inkcazelo yeKit:

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo ukukhulula ngokukhawuleza i-DNA ye-genomic kwi-zebrafish kunye nezinye izihlunu zentlanzi zamanzi ahlambulukileyo, ama-fins omsila okanye iisampuli zeqanda leentlanzi kwiimpendulo ze-PCR, ngoko ke ifaneleka ngakumbi kuvavanyo lwemfuzo olukhulu.Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwe ngaphakathi kwe-10-30 imizuzu kwi-65 ° C.Azikho ezinye iinkqubo ezifana neprotheyini kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA ungasetyenziswa njenge template ye-PCR reaction.

I-2 × PCR Easy TM Mix inokunyamezela okuqinileyo kwi-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampuli ukuba ivavanywe njenge template yokukhulisa ngokufanelekileyo kunye nokucacileyo.Le reagent iqulethe ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer kunye stabilizer.Isetyenziswe ngokubambisana ne-lysis buffer inokukhawuleza kwaye ibone iisampulu ngokulula, kwaye ineempawu zobuntununtunu obuphezulu, ukuchaneka okunamandla, kunye nokuzinza okulungileyo.

I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Undoqo kwimpumelelo yethu “nguMgangatho oLungileyo weMveliso, iXabiso eliQinisekileyo kunye neNkonzo eFanelekileyo” ye-China Wholesale China Lonke ixesha loNyaka loBulunga oBonelelo lwePCR, “Ubunjani”, “ukunyaniseka” kunye “nenkonzo” ngumgaqo wethu.Ukunyaniseka kwethu kunye nokuzinikela kwethu kuhlala kunentlonipho kwinkonzo yakho.Thetha nathi Namhlanje Ngedatha eyongezelelekileyo, qhagamshelana nathi ngoku.
Isitshixo kwimpumelelo yethu "nguMgangatho oMhle weMveliso, iXabiso eliQinisekileyo kunye neNkonzo eFanelekileyo" yeUmgangatho ogqwesileyo we-PCR, China Lonke ixesha PCR, Iqela lethu liwazi kakuhle iimfuno zemarike kumazwe ahlukeneyo, kwaye liyakwazi ukubonelela ngezinto ezifanelekileyo ezisemgangathweni ngamaxabiso angcono kwiimarike ezahlukeneyo.Inkampani yethu sele imisele iqela eliqeqeshiweyo, eliyilayo nelinoxanduva lokuphuhlisa abathengi ngomgaqo wokuphumelela okuninzi.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I	Buffer FP
	I-Foregene Protease
	I-6 × i-DNA yokulayisha i-Buffer
Icandelo II	2×PCR Easy^TMXuba
Imiyalelo

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-1mg ye-caudal fin yentlanzi okanye amaqanda entlanzi ali-10 anokuhlolwa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Iintlanzi zeZebra kunye nezinye iintlanzi zamanzi ahlambulukileyo.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

wk-flw-zebra-fish-direct-PCR

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

v IReagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

vReagent 6×DNA Loading Buffer ingagcinwa kwi 4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-vReagent 2 × PCR Easy TM Mix, ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (sebenzisa kwiintsuku ze-10).

Isitshixo kwimpumelelo yethu "nguMgangatho oMhle weMveliso, iXabiso eliQinisekileyo kunye neNkonzo eFanelekileyo" ye-China Wholesale China Lonke Ixesha loMgangatho oLona mgangatho we-PCR 185/70r13, "Umgangatho", "ukunyaniseka" kunye "nenkonzo" ngumgaqo wethu.Ukunyaniseka kwethu kunye nokuzinikela kwethu kuhlala kunentlonipho kwinkonzo yakho.Thetha nathi Namhlanje Ngedatha eyongezelelekileyo, qhagamshelana nathi ngoku.
I-China Ihoseyili yaseTshayina Lonke ixesha le-PCR, i-PCR yoMgangatho oPhezulu, Iqela lethu liwazi kakuhle iimfuno zemarike kumazwe ahlukeneyo, kwaye liyakwazi ukubonelela ngezinto ezisemgangathweni ezifanelekileyo ngamaxabiso angcono kwiimarike ezahlukeneyo.Inkampani yethu sele imisele iqela eliqeqeshiweyo, eliyilayo nelinoxanduva lokuphuhlisa abathengi ngomgaqo wokuphumelela okuninzi.

Ngaphambili: Umzi-mveliso unikezela ngokuthe ngqo eTshayina Rna DNA Nucleic Acid Extraction Reagent

Okulandelayo: Uyilo oludumileyo lwaseTshayina oluLahliweyo lweSampuli yeThubhu yeSampuli eneSwabs yeSixhobo soVavanyo

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