China ngqo PCR kit uMvelisi kunye noMboneleli |I-Foregene

ikhithi PCR ngqo

Inkcazelo yeKit:

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo ukukhulula ngokukhawuleza i-DNA ye-genomic kwi-zebrafish kunye nezinye izihlunu zentlanzi zamanzi ahlambulukileyo, ama-fins omsila okanye iisampuli zeqanda leentlanzi kwiimpendulo ze-PCR, ngoko ke ifaneleka ngakumbi kuvavanyo lwemfuzo olukhulu.Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwe ngaphakathi kwe-10-30 imizuzu kwi-65 ° C.Azikho ezinye iinkqubo ezifana neprotheyini kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA ungasetyenziswa njenge template ye-PCR reaction.

I-2 × PCR Easy TM Mix inokunyamezela okuqinileyo kwi-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampuli ukuba ivavanywe njenge template yokukhulisa ngokufanelekileyo kunye nokucacileyo.Le reagent iqulethe ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer kunye stabilizer.Isetyenziswe ngokubambisana ne-lysis buffer inokukhawuleza kwaye ibone iisampulu ngokulula, kwaye ineempawu zobuntununtunu obuphezulu, ukuchaneka okunamandla, kunye nokuzinza okulungileyo.

I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Sihlala siwenza rhoqo umoya wethu “wokusungula izinto ezintsha ezizisa uphuhliso, umgangatho ophezulu wokuphila, intengiso yoLawulo kunye nenzuzo yokuthengisa, Amanqaku etyala atsala abathengi ngekiti yePCR ethe ngqo, besijonge phambili ukusebenzisana nawo onke amathuba avela ekhaya naphesheya.Ngaphezu koko, ukuzaliseka kwabathengi kukufuna kwethu ngonaphakade.
Sihlala siwuphumeza umoya wethu wokuba “Utshintsho oluzisa uphuhliso, Umgangatho ophezulu wokwenza ukutya okuthile, intengiso yoLawulo kunye nenzuzo yokuthengisa, Amanqaku etyala atsala abathengiIKit yePC ethe ngqo, Sijonge phambili ekusebenzisaneni ngokusondeleyo nawe kwiinzuzo zethu ezifanayo kunye nophuhliso oluphezulu.Siqinisekise umgangatho, ukuba abathengi abanelisekanga ngumgangatho weemveliso, unokubuyela ngaphakathi kwe-7days kunye namazwe abo okuqala.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I	Buffer FP
	I-Foregene Protease
	I-6 × i-DNA yokulayisha i-Buffer
Icandelo II	2×PCR Easy^TMXuba
Imiyalelo

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-1mg ye-caudal fin yentlanzi okanye amaqanda entlanzi ali-10 anokuhlolwa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Iintlanzi zeZebra kunye nezinye iintlanzi zamanzi ahlambulukileyo.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

wk-flw-zebra-fish-direct-PCR

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

v IReagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

vReagent 6×DNA Loading Buffer ingagcinwa kwi 4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-vReagent 2 × PCR Easy TM Mix, ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (sebenzisa kwiintsuku ze-10).

Sihlala siwenza rhoqo umoya wethu “wokuzisa uphuhliso, umgangatho ophezulu wokuphila, intengiso yolawulo kunye nenzuzo yokuthengisa, amanqaku etyala atsala abathengi beBig Discount China Cdv AG The Canine Distemper Virus Antigen Rapid Test Kit, besijonge phambili ukusebenzisana nawo onke amathemba avela ekhaya naphesheya.Ngaphezu koko, ukuzaliseka kwabathengi kukufuna kwethu ngonaphakade.
I-Big Discount China Card Test, i-Strips yoVavanyo, sijonge phambili ukusebenzisana ngokusondeleyo nawe kwiinzuzo zethu ezifanayo kunye nophuhliso oluphezulu.Siqinisekise umgangatho, ukuba abathengi abanelisekanga ngumgangatho weemveliso, unokubuyela ngaphakathi kwe-7days kunye namazwe abo okuqala.

Ngaphambili: Umgangatho ophezulu / imfezeko iyonke ikhithi yokuzahlula ye-RNA

Okulandelayo: Umzi-mveliso ngokuthe ngqo unikezelo lwaseTshayina oluPhezulu lwaBakhweli baMatayara PCR Trailer iTire 155r12 185r14c 195r14c 205/55r16 215/45r17

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