Amava olawulo lweeprojekthi ezininzi kakhulu kunye ne-1 ukuya kwimodeli yomboneleli nje enye yenza ukubaluleka okuphezulu konxibelelwano lwenkampani kunye nokuqonda kwethu lula ukulindela kwakho kwiFactory ethengisa kakhulu iChina Magnetic Bead Method Nucleic Acid Extraction Kits Rna Isolation DNA Purification Kit Lab Reagent, Njengeqela elinamava nathi samkela imiyalelo elungiselelweyo.Eyona njongo iphambili yequmrhu lethu kukuhlala kukuphuhlisa inkumbulo eyanelisayo kubo bonke abathengi, kunye nokuseka ubudlelwane beshishini bexesha elide lokuwina.
Amava olawulo lweeprojekthi ezininzi kakhulu kunye ne-1 ukuya kwimodeli yomboneleli enye yenza ukubaluleka konxibelelwano lwenkampani kunye nokuqonda kwethu lula ulindelo lwakho.Ukuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, China Universal Rna DNA Reagents, Ngamandla aqiniweyo kunye nekhredithi ethembekileyo ngakumbi, besilapha ukunceda abathengi bethu ngokubonelela ngomgangatho ophezulu kunye nenkonzo, kwaye siyayibulela ngokunyanisekileyo inkxaso yakho.Siza kuzama ukugcina igama lethu elikhulu njengoyena mthengisi ubalaseleyo kwihlabathi.Ukuba unayo nayiphi na imibuzo okanye izimvo, kufuneka uqhagamshelane nathi ngokukhululekileyo.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides okanye umxholo we-polyphenols.Inika ikholomu ye-DNA-Cleaning enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Ngokukodwa ifanele ukucocwa kwe-RNA kwiisampuli zezityalo ze-polysaccharides kunye ne-polyphenols.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
∎ Isampulu: Izihlunu zezityalo ezitsha okanye ezikhenkcezisiweyo zeepolysaccharides neepolyphenols
∎ Umthamo: 50mg izicubu zezityalo
■ Ubuninzi bomthamo wokubopha i-RNA yekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA epheleleyo kwiisampuli zezicubu zezityalo ezitsha okanye ezikhenkcezisiweyo (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye nomxholo ophezulu we-polysaccharide kunye ne-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ugcino kunye nobomi beShelf

Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
I-Buffer PSL1 inokubekwa kwi-4℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (kucetyiswa ukuba uyifake ngexesha elifanayo lokulinga) .Amava olawulo lweeprojekthi ezininzi kakhulu kunye ne-1 ukuya kwimodeli enye yomnikezeli yenza ukubaluleka okuphezulu konxibelelwano lwenkampani kunye nokuqonda kwethu ngokulula okulindelweyo kwiFactory ethengisa kakhulu i-China Magnetic Bead Method I-Nuclear ye-DNA ye-Nuclear ye-Nuclear ye-RedD iqela sikwazamkela iiodolo ezilungiselelweyo.Eyona njongo iphambili yequmrhu lethu kukuhlala kukuphuhlisa inkumbulo eyanelisayo kubo bonke abathengi, kunye nokuseka ubudlelwane beshishini bexesha elide lokuwina.
Ifektri ithengiswa kakhuluChina Universal Rna DNA Reagents, Ukuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, Ngamandla aqiniweyo kunye nekhredithi ethembekileyo ngakumbi, besilapha ukunceda abathengi bethu ngokubonelela ngomgangatho ophezulu kunye nenkonzo, kwaye siyayibulela ngokunyanisekileyo inkxaso yakho.Siza kuzama ukugcina igama lethu elikhulu njengoyena mthengisi ubalaseleyo kwihlabathi.Ukuba unayo nayiphi na imibuzo okanye izimvo, kufuneka uqhagamshelane nathi ngokukhululekileyo.