Ukusebenzisa inkqubo epheleleyo yenzululwazi yolawulo lomgangatho olungileyo, umgangatho olungileyo kakhulu kunye nokholo oluphezulu, siphumelele ukuma okuhle kwaye sahlala kule ngqeqesho yexabiso eliphantsi lomzi-mveliso China Long Taq DNA Polymerase, Long PCR, Samkela ngokunyanisekileyo zonke iindwendwe ukuba zilungiselele ubudlelwane benkampani kunye nathi malunga nesiseko semiba efanelekileyo efanayo.Kufuneka uqhagamshelane nathi ngoku.Ungafumana impendulo yethu yezakhono ngaphakathi kwe-8 iiyure ezininzi.
Sisebenzisa inkqubo epheleleyo yenzululwazi yolawulo lomgangatho olungileyo, umgangatho olungileyo kakhulu kunye nokholo oluphezulu, siphumelele ukuma okuhle kwaye sibambe olu qeqeshoChina PCR Ukwandiswa, Qpcr, Inkampani yethu igubungela indawo ye-20, 000 yeemitha square.Sinabasebenzi abangaphezu kwama-200, iqela lobuchwephesha leengcali, amava eminyaka eli-15, umsebenzi ogqwesileyo, umgangatho ozinzile nothembekileyo, ixabiso elikhuphisanayo kunye nomthamo owaneleyo wemveliso, le yindlela esenza ngayo abathengi bethu bomelele.Ukuba unayo nayiphi na imibuzo, nceda ungathandabuzi ukuqhagamshelana nathi.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-1mg ye-caudal fin yentlanzi okanye amaqanda entlanzi ali-10 anokuhlolwa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Iintlanzi zeZebra kunye nezinye iintlanzi zamanzi ahlambulukileyo.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

v IReagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

vReagent 6×DNA Loading Buffer ingagcinwa kwi 4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-vReagent 2 × PCR Easy TM Mix, ukuba isetyenziswa rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (ukusetyenziswa ngaphakathi kweentsuku ze-10) . Ukusebenzisa inkqubo yolawulo olusemgangathweni olulungileyo lwenzululwazi, umgangatho omhle kakhulu kunye nokholo oluphezulu, siphumelele ukuma okuhle kwaye sithathe le ngqeqesho yexabiso eliphantsi kwe-China Long Taq DNA Polymerase, i-PCR ende, Samkela ngokunyanisekileyo ubudlelwane benkampani kunye neendwendwe ngokunyanisekileyo.Kufuneka uqhagamshelane nathi ngoku.Ungafumana impendulo yethu yezakhono ngaphakathi kwe-8 iiyure ezininzi.
ixabiso eliphantsi lomzi-mvelisoChina PCR Ukwandiswa, Qpcr, Inkampani yethu igubungela indawo ye-20, 000 yeemitha square.Sinabasebenzi abangaphezu kwama-200, iqela lobuchwephesha leengcali, amava eminyaka eli-15, umsebenzi ogqwesileyo, umgangatho ozinzile nothembekileyo, ixabiso elikhuphisanayo kunye nomthamo owaneleyo wemveliso, le yindlela esenza ngayo abathengi bethu bomelele.Ukuba unayo nayiphi na imibuzo, nceda ungathandabuzi ukuqhagamshelana nathi.