Yindlela elungileyo yokuphucula iimveliso zethu kunye nezisombululo kunye nokulungiswa.Injongo yethu iya kuba kukwakha izisombululo zokuyila kubathengi abanamava amnandi kwiFactory ethengisa i-China yoPhando Sebenzisa iMagnetic Viral Rna Nucleic Acid yokucoca iReagent Universal Extraction Kits, Samkela ngokufudumeleyo amaqabane amashishini amancinci asuka kuzo zonke iindlela zokuphila, sinethemba lokuseka ishishini elinobuhlobo kunye nentsebenziswano linxibelelana nawe kwaye uzuze injongo yokuphumelela.
Yindlela elungileyo yokuphucula iimveliso zethu kunye nezisombululo kunye nokulungiswa.Injongo yethu iya kuba kukwakha izisombululo zokuyila kubathengi abanamava amahleUkuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, China Universal Rna DNA Reagents, Umgangatho ogqwesileyo kunye nowokuqala weendawo ezisecaleni yeyona nto ibaluleke kakhulu kwezothutho.Sisenokunamathela ekunikezeni iindawo zoqobo nezisemgangathweni nokuba yingeniso encinci esiyifumeneyo.UThixo uya kusisikelela ukuba senze ishishini lobubele ngonaphakade.

Iinkcukacha

50 Preps, 200 Preps

I-Viral RNA I-Nucleic Acid Ukucoca I-Isolation Kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA yentsholongwane kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid, kunye ne-cell culture supernatant.Ikiti yongeza ngokukodwa i-Linear Acrylamide, enokuthi ibambe ngokulula ixabiso elincinci le-RNA kwiisampuli.IKholam ye-RNA Kuphela inokubopha i-RNA ngokufanelekileyo.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Ikhithi yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer viRW1 kunye ne-Buffer viRW2 inokuqinisekisa ukuba i-viral nucleic acid efunyenweyo ayinaprotheyini, i-nuclease okanye enye into engcolileyo, enokusetyenziswa ngokuthe ngqo kwimifuniselo yebhayoloji yemolekyuli esezantsi.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Isivuno esiphezulu se-nucleic acid: I-DNA / RNA-iKholamu kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-DNA kunye ne-RNA.
■ Umthamo omkhulu wokusetyenzwa kwesampulu: ukuya kuthi ga kwi-200μl iisampulu zisenokwenziwa ngexesha ngalinye.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-20.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukukhutshwa kunye nokucocwa kwe-viral nucleic acid kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid kunye ne-cell culture supernatant.

Ukuhamba komsebenzi

Umzobo

Ugcino kunye nobomi beShelf

■ Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
■ Isisombululo seLinear Acrylamide sinokugcinwa kwiqondo lobushushu legumbi kangangeentsuku ezisi-7;emva kokufumana ikiti, nceda uyikhuphe kwaye uyigcine kwi-20°C.
■ Emva kokongeza i-Linear Acrylamide kwi-Buffer DRL, inokugcinwa kwi-2-8 ° C ukuya kwi-48h.Nceda usebenzise isisombululo esenziwe eselenziwe.Yindlela elungileyo yokuphucula iimveliso zethu kunye nezisombululo kunye nokulungiswa.Injongo yethu iya kuba kukwakha izisombululo zokuyila kubathengi abanamava amnandi kwiFactory ethengisa i-China yoPhando Sebenzisa iMagnetic Viral Rna Nucleic Acid yokucoca iReagent Universal Extraction Kits, Samkela ngokufudumeleyo amaqabane amashishini amancinci asuka kuzo zonke iindlela zokuphila, sinethemba lokuseka ishishini elinobuhlobo kunye nentsebenziswano linxibelelana nawe kwaye uzuze injongo yokuphumelela.
Ukuthengiswa kweFactoryChina Universal Rna DNA Reagents, Ukuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, Umgangatho ogqwesileyo kunye nowokuqala weendawo ezisecaleni yeyona nto ibaluleke kakhulu kwezothutho.Sisenokunamathela ekunikezeni iindawo zoqobo nezisemgangathweni nokuba yingeniso encinci esiyifumeneyo.UThixo uya kusisikelela ukuba senze ishishini lobubele ngonaphakade.