Ngokwenene luxanduva lwethu ukwanelisa iimfuno zakho kwaye sikukhonze ngokufanelekileyo.Ukuzaliseka kwakho ngowona mvuzo wethu mkhulu.Sijonge phambili kuhambo lwakho lwenkqubela phambili edibeneyo yoBonelelo lwaseTshayina iNyathelo elinye lokuFundela iVirus yeDengue (qPCR-Probe), Sikwangumzi-mveliso we-OEM onyuliweyo kwiimpawu ezininzi zentengiso zehlabathi.Wamkelekile ukuba uthethe nathi ukwenzela uthethathethwano kunye nentsebenziswano.
Ngokwenene luxanduva lwethu ukwanelisa iimfuno zakho kwaye sikukhonze ngokufanelekileyo.Ukuzaliseka kwakho ngowona mvuzo wethu mkhulu.Sijonge phambili ekuhambeni kwakho kwinkqubela phambili edibeneyo yeChina Dengue Virus Ixesha lokwenyani PCR Kit, IVirusi yeDengue Qpcr Kit, Ukuba zizisombululo eziphezulu zefektri yethu, uluhlu lwethu lwezisombululo luye lwavavanywa kwaye lwasiphumelela iziqinisekiso zegunya.Kwiiparamitha ezongezelelweyo kunye neenkcukacha zoluhlu lwezinto, qiniseka ukucofa iqhosha ukufumana ulwazi olongezelelweyo.

Iinkcukacha

50×20μl rxns, 200×20μl rxns, 1000×20μl rxns, 2000×20μl rxns

I-2 × Real PCR Easy TM Mix-SYBR enikezelwa yi-Real Time PCR Easy TM-SYBR I-Green I kit yinkqubo entsha ye-premix esebenzisa i-SYBR Green I kwi-Real Time PCR yokuphendula i-amplification, enokuphucula kakhulu imveliso ethile kunye novelwano lokuphendula.I-ROX inikezelwa njengedayi yolawulo lwangaphakathi.Ukuqina kwe-fluorescence yale khithi ngama-3-5 amaxesha eemveliso ezifanayo, ezinokuthi zibe novakalelo kwaye zibonise ngokucacileyo ukuxinwa kwe-template ye-DNA ekujoliswe kuyo.

I-2 × I-PCR yokwenene i-Easy TM Mix-SYBR iqulethe i-Foregene ekhethekileyo eshushu-isiqalo se-Taq DNA Polymerase.Xa kuthelekiswa nee-enzymes ze-Taq eziqhelekileyo, ineengenelo zokusebenza kwe-amplification ephezulu, isakhono esithile esinamandla sokukhulisa kunye nesantya esisezantsi sokungahambelani.Inokunciphisa ukukhuliswa okungangqaliyo kunye nokuphucula ukuchaneka kwe-PCR.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ilula—I-2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza

■ I-Specific-optimized buffer kunye ne-hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.

■ Uvakalelo oluphezulu-lukwazi ukubona iikopi ezisezantsi zetemplate

■ Ukuguquguquka okuhle—okuhambelana nezixhobo ezininzi zePCR zexesha lokwenyani

Isicelo seKit

Uhlalutyo lwe-qPCR

Ukuhamba komsebenzi

Umzobo

Ugcino kunye nobomi beShelf

Le khithi kufuneka igcinwe kude nokukhanya kwaye kufuneka igcinwe ku -20 ℃.Ukuba isetyenziswa rhoqo, inokugcinwa kwi-4℃ ixesha elifutshane (iintsuku ezili-10). Ngokwenene luxanduva lwethu ukwanelisa iimfuno zakho kwaye sikukhonze ngokufanelekileyo.Ukuzaliseka kwakho ngowona mvuzo wethu mkhulu.Sijonge phambili kuhambo lwakho lwenkqubela phambili edibeneyo yoBonelelo lwaseTshayina iNyathelo elinye lokuFundela iVirus yeDengue (qPCR-Probe), Sikwangumzi-mveliso we-OEM onyuliweyo kwiimpawu ezininzi zentengiso zehlabathi.Wamkelekile ukuba uthethe nathi ukwenzela uthethathethwano kunye nentsebenziswano.
Ubonelelo lwefektriChina Dengue Virus Ixesha lokwenyani PCR Kit, IVirusi yeDengue Qpcr Kit, Ukuba zizisombululo eziphezulu zefektri yethu, uluhlu lwethu lwezisombululo luye lwavavanywa kwaye lwasiphumelela iziqinisekiso zegunya.Kwiiparamitha ezongezelelweyo kunye neenkcukacha zoluhlu lwezinto, qiniseka ukucofa iqhosha ukufumana ulwazi olongezelelweyo.