Sikwayingcali ekuphuculeni izinto zolawulo kunye nendlela ye-QC ukuze sikwazi ukugcina umda owoyikekayo ngaphakathi kwishishini elincinci elikhuphisanayo kwisampulu yasimahla ye-CE / FDA eYamkelweyo ye-Magnetic Beads Indlela yeDNA / iRna Nucleic Acid Extraction Reagent Kits (i-Pre-packed Magnetic Beads), Ukuqonda unxibelelwano lwethu lwamazwe ngamazwe lunenzuzo yokuthengisa kumazwe aphesheya. , ukuhanjiswa ngokukhawuleza, olona mgangatho ulungileyo kunye nentsebenziswano yexesha elide.
Sikwayingcali ekuphuculeni ulawulo lwezinto kunye nendlela ye-QC ukuze sikwazi ukugcina umda owoyikekayo ngaphakathi kwishishini elincinci elikhuphisana ngokuqatha.China Nucleic Acid, China Nucleic Acid Abavelisi, Imveliso yethu ithunyelwe kumazwe angaphezu kwama-30 kunye nemimandla njengomthombo wesandla sokuqala kunye nexabiso eliphantsi.Samkela ngokunyanisekileyo abathengi abasuka ekhaya nakwamanye amazwe ukuba beze kuthethathethana nathi ngoshishino.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 ingagcinwa kwi-4 ℃ kwinyanga ye-1 emva kokufaka i-β- mercaptoethanol (ukhetho) .Sikwayingcali ekuphuculeni izinto zolawulo kunye nendlela ye-QC ukwenzela ukuba sigcine umda onobungozi ngaphakathi kwishishini elincinci elikhuphisana ngokukrakra kwisampuli yaMahala ye-China CE / FDA Approved Magnetic Acculection Nuclear-Method. iintengiso), Ukuqonda izibonelelo ezihambelanayo, ishishini lethu likhulisa amaqhinga ethu onxibelelwano lwehlabathi malunga nonxibelelwano nabathengi baphesheya, ukuhanjiswa ngokukhawuleza, olona mgangatho ulungileyo kunye nentsebenziswano yexesha elide.
Isampulu yasimahla yeChina Nucleic Acid, China Nucleic Acid Abavelisi, Imveliso yethu ithunyelwe kumazwe angaphezu kwama-30 kunye nemimandla njengomthombo wesandla sokuqala kunye nexabiso eliphantsi.Samkela ngokunyanisekileyo abathengi abasuka ekhaya nakwamanye amazwe ukuba beze kuthethathethana nathi ngoshishino.