I-China iMgangatho oLungileyo waseChina iBioer Biospin DNA / iRna Extraction Kit 50t uMenzi weReagent kunye noMboneleli |I-Foregene

Umgangatho oLungileyo waseTshayina iBiospin DNA / iRna Extraction Kit 50t Reagent

Inkcazelo yeKit:

Le khithi isebenzisa itekhnoloji ye-RT PCR yexesha lokwenyani (rRT-PCR) ukufunyaniswa komgangatho we-SARS-CoV-2 (i-ORF1ab gene kunye ne-N gene) ye-nucleic acids kwiisampulu ze-nasopharyngeal okanye ze-oropharyngeal swab, nge-multiplex PCR fluorescent probe method. ukufunyanwa ngokukhawuleza okukhulu.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukuphucula rhoqo inkqubo yolawulo ngomthetho othi "onyanisekileyo, ukholo oluhle kakhulu kunye nomgangatho ophezulu zisisiseko sophuhliso lweshishini", sithatha ngokubanzi isiseko seemveliso ezinxulumene namazwe ngamazwe, kwaye sihlala siphuhlisa iimveliso ezintsha ukuzalisekisa iminxeba yabathengi boMgangatho oLungileyo waseTshayina Bioer Biospin DNA / Rna.Ikhithi yokutsalwaI-50t Reagent, Ukususela ekusekweni kweyunithi yokuvelisa, ngoku sizibophelele kwinkqubela phambili yempahla entsha.Kunye nesantya sezentlalo nezoqoqosho, siza kuqhubeka ukuqhubela phambili umoya “wokugqwesa okuphezulu, ukusebenza kakuhle, ukusungula izinto ezintsha, imfezeko”, kwaye sihlale nomgaqo wokusebenza “wetyala ekuqaleni, umthengi wokuqala, umgangatho olungileyo obalaseleyo”.Siza kuvelisa ikamva elihle elibonakalayo kwimveliso yeenwele kunye namaqabane ethu.
Ukuphucula rhoqo inkqubo yolawulo ngomthetho othi "onyanisekileyo, ukholo oluhle kakhulu kunye nomgangatho ophezulu zisisiseko sophuhliso lweshishini", sithatha ngokubanzi isiseko seemveliso ezinxulumene namazwe ngamazwe, kwaye sihlala siphuhlisa iimveliso ezintsha ukuzalisekisa iminxeba yabathengi.China Dbiozol Genomic DNA, Ikhithi yokutsalwa, Ukholo lwethu kukunyaniseka kuqala, ngoko ke sibonelela ngezinto ezikumgangatho ophezulu kubathengi bethu.Ngenene ndiyathemba ukuba sinokuba ngamaqabane oshishino.Sikholelwa ukuba sinokuseka ubudlelwane bexesha elide kunye nomnye.Ungaqhagamshelana nathi ngokukhululekileyo ngolwazi oluthe kratya kunye noluhlu lwamaxabiso lweemveliso zethu kunye nezisombululo !

Iinkcukacha

48 Rxns, 96 Rxns

Hayi.	Icandelo	Isixa				Amacandelo aphambili
Hayi.	Icandelo	48Rxns		96 Rxns		Amacandelo aphambili
1	I-agent yokukhululwa kwe-Nucleic acid	1.4mL / ityhubhu	2 iityhubhu	5.3 mL/ibhotile	1 ibhotile	I-surfactant
2	Umkhuseli we-RNA	27 μL/ityhubhu	ityhubhu e-1	53 μL/ityhubhu	ityhubhu e-1	I-RNase inhibitor
3	Isisombululo sokusabela kwe-SARS-CoV-2	800 μL/ityhubhu	ityhubhu e-1	1600 μL/ityhubhu	ityhubhu e-1	Iprimer, probe, reaction buffer, dNTP
4	Umxube we-enzyme ye-SARS-CoV-2	80 μL/ityhubhu	ityhubhu e-1	160 μL/ityhubhu	ityhubhu e-1	Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV
5	Ulawulo olulungileyo lwe-SARS-CoV-2	100 μL/ityhubhu	ityhubhu e-1	100 μL/ityhubhu	ityhubhu e-1	I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA
6	Ulawulo olubi lwe-SARS-CoV-2	1200 μL/ityhubhu	ityhubhu e-1	1200 μL/ityhubhu	ityhubhu e-1	TE buffer

Iimpawu&izinto eziluncedo

Ngokukhawuleza kwaye kulula.
Gwema ilahleko ye-nucleic acid.
Umda wokubona okuphantsi kunye novakalelo oluphezulu.
Imfuneko yezixhobo eziphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu.

Ukuqonda ukuqonda kunokufikelela kwinqanaba eliphantsi njengeekopi ze-500 / mL).

GCE/mL	Ct ixabiso
GCE/mL	Njini	Uhlobo lweORF1ab
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N / A	N / A
2.00E-01	N / A	N / A
NC	N / A	N / A

Igophe lokukhulisa umgca

igophe lokukhulisa-RT-qPCR

Kusetyenziswa kwanjani

1. Isixhobo esisebenzayo

i-covid-19 ikiti yokubona i-nucleic acid1

2. Inkqubo yokufumanisa

i-covid-19 ikiti yokubona i-nucleic acid2

Isampulu yokusetyenzwa: Isampuli ye-Naopharyngeal okanye i-oropharyngeal swab (inyathelo eli-1 kuphela) (Linda imizuzu eyi-10)

PCR amplificaton(<=55min)

Umsebenzi:

i-covid-19 ikiti yokubona i-nucleic acid4

Ugcino

itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;

yokugcina
ugcino2

Beka ubomi kwishelufa:1 unyaka

Ukuphucula rhoqo inkqubo yolawulo ngomthetho othi "onyanisekileyo, ukholo oluhle kakhulu kunye nomgangatho ophezulu zisisiseko sophuhliso lweshishini", sithatha ngokubanzi isiseko seemveliso ezinxulumene namazwe ngamazwe, kwaye sihlala siphuhlisa iimveliso ezintsha ukuzalisekisa iminxeba yabathengi boMgangatho oLungileyo waseTshayina Bioer Biospin DNA / Rna.Ikhithi yokutsalwaI-50t Reagent, Ukususela ekusekweni kweyunithi yokuvelisa, ngoku sizibophelele kwinkqubela phambili yempahla entsha.Kunye nesantya sezentlalo nezoqoqosho, siza kuqhubeka ukuqhubela phambili umoya “wokugqwesa okuphezulu, ukusebenza kakuhle, ukusungula izinto ezintsha, imfezeko”, kwaye sihlale nomgaqo wokusebenza “wetyala ekuqaleni, umthengi wokuqala, umgangatho olungileyo obalaseleyo”.Siza kuvelisa ikamva elihle elibonakalayo kwimveliso yeenwele kunye namaqabane ethu.
Umgangatho olungileyoChina Dbiozol Genomic DNA, Ikhithi yokuSebenza, ukholo lwethu kukunyaniseka kuqala, ngoko ke sibonelela ngezinto ezikumgangatho ophezulu kubathengi bethu.Ngenene ndiyathemba ukuba sinokuba ngamaqabane oshishino.Sikholelwa ukuba sinokuseka ubudlelwane bexesha elide kunye nomnye.Ungaqhagamshelana nathi ngokukhululekileyo ngolwazi oluthe kratya kunye noluhlu lwamaxabiso lweemveliso zethu kunye nezisombululo !

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