Ugxininiso lwethu kufuneka lube kukuhlanganisa kunye nokuphucula umgangatho kunye nokulungiswa kweemveliso zangoku, okwangoku gqolo ukuseka iimveliso ezintsha ukuhlangabezana neemfuno zabathengi ezizodwa zomgangatho oLungileyo weChina Column Nucleic Acid Rna Isolation Kit, isizukulwana sesithathu, Nganye yeembono kunye nezicwangciso ziya kuxatyiswa kakhulu!Intsebenziswano entle ingasenza uphuculo kuthi sobabini sikhule ngcono!
Ugxininiso lwethu kufuneka lube kukuhlanganisa kunye nokuphucula umgangatho kunye nokulungiswa kweemveliso zangoku, okwangoku sihlala siseka iimveliso ezintsha ukuhlangabezana neemfuno zabathengi abakhethekileyoChina Isolation Kit, I-Pcr Rna Extraction Reagent, Sithobela ukunyaniseka, okusebenzayo, okusebenzayo kokuphumelela ukuphumelela ubuthunywa kunye nefilosofi yoshishino ejolise ebantwini.Umgangatho ogqwesileyo, ixabiso elifanelekileyo kunye nokwaneliseka kwabathengi zihlala zilandelwa!Ukuba unomdla kwizinto zethu, zama nje ukunxibelelana nathi ngeenkcukacha ezithe kratya!

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Ugxininiso lwethu kufuneka lube kukuhlanganisa kunye nokuphucula umgangatho kunye nokulungiswa kweemveliso zangoku, okwangoku sihlala siseka iimveliso ezintsha ukuhlangabezana neemfuno zabathengi ezizodwa zomgangatho oLungileyo weChina Magnetic Particles Nucleic Acid Rna Isolation Kit, Imbono nganye kunye nezicwangciso ziya kuxatyiswa kakhulu!Intsebenziswano entle ingasenza uphuculo kuthi sobabini sikhule ngcono!
Umgangatho olungileyoChina Isolation Kit, I-PCR Rna Extraction Reagent, Sibambelela ekunyanisekeni, okusebenzayo, okusebenzayo kokuphumelela ukuphumelela ubuthunywa kunye nefilosofi yoshishino ejolise ebantwini.Umgangatho ogqwesileyo, ixabiso elifanelekileyo kunye nokwaneliseka kwabathengi zihlala zilandelwa!Ukuba unomdla kwizinto zethu, zama nje ukunxibelelana nathi ngeenkcukacha ezithe kratya!