Ukusungula izinto ezintsha, umgangatho olungileyo kunye nokuthembeka zizinto ezixabisekileyo ezingundoqo zeshishini lethu.Le migaqo namhlanje eyongezelelekileyo kunangaphambili isisiseko sempumelelo yethu njengombutho wamazwe ngamazwe ophakathi komgangatho oLungileyo oPhakamileyo weXesha lokwenyani iPCR Kit Hotstart Taq DNA Polymerase Power Green Qpcr Mix, Inkampani yethu izinikele ekuboneleleni abathengi ngeemveliso ezikumgangatho ophezulu nezizinzileyo ngexabiso elikhuphisanayo, ukwenza wonke umxhasi aneliseke ngeemveliso kunye neenkonzo zethu.
Ukusungula izinto ezintsha, umgangatho olungileyo kunye nokuthembeka zizinto ezixabisekileyo ezingundoqo zeshishini lethu.Le mithetho-siseko namhlanje eyongezelelekileyo kunangaphambili yenza isiseko sempumelelo yethu njengombutho wehlabathi osebenzayo ophakathi komlinganiselo weI-China Taq DNA Polymerase kunye ne-Qpcr, Xa unomdla kuyo nayiphi na into yethu elandelayo ukujonga uluhlu lwemveliso yethu, nceda uzive ukhululekile ukuqhagamshelana nathi ngemibuzo.Uya kukwazi ukusithumelela ii-imeyile kwaye uqhagamshelane nathi ukuze sibonisene kwaye siya kukuphendula ngokukhawuleza ukuba sikwazi ukwenza njalo.Ukuba kufanelekile, ungafumana idilesi yethu kwiwebhusayithi yethu kwaye uze kwishishini lethu.okanye ulwazi olongezelelweyo lwezinto zethu wedwa.Sisoloko sikulungele ukwakha ubudlelwane bentsebenziswano obude nobuzinzileyo nabo nabaphi na abathengi abanokubakho kwiindawo ezinxulumeneyo.

Iinkcazelo Kit

I-2 × I-PCR yangempela elula^TMUmxube-SYBR obonelelwa yi-Real Time PCR Easy^TM-SYBR Green I kit yinkqubo entsha ye-premix esebenzisa i-SYBR Green I yeReality Time PCR reactions yokukhulisa, enokuphucula kakhulu imveliso ethile kunye nobuntununtunu bokusabela.I-ROX inikezelwa njengedayi yolawulo lwangaphakathi.Ukuqina kwe-fluorescence yale khithi ngama-3-5 amaxesha eemveliso ezifanayo, ezinokuthi zibe novakalelo kwaye zibonise ngokucacileyo ukuxinwa kwe-template ye-DNA ekujoliswe kuyo.

I-2 × I-PCR yangempela elula^TMI-Mix-SYBR iqulethe i-Foregene eshushu-isiqalo esisodwa se-Taq DNA Polymerase.Xa kuthelekiswa nee-enzymes ze-Taq eziqhelekileyo, ineengenelo zokusebenza kwe-amplification ephezulu, isakhono esithile esinamandla sokukhulisa kunye nesantya esisezantsi sokungahambelani.Inokunciphisa ukukhuliswa okungangqaliyo kunye nokuphucula ukuchaneka kwe-PCR.

Iinkcukacha

Iimpawu&izinto eziluncedo

■ Ilula—I-2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza

■ I-Specific-optimized buffer kunye ne-hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.

■ Uvakalelo oluphezulu-lukwazi ukubona iikopi ezisezantsi zetemplate

■ Ukuguquguquka okuhle—okuhambelana nezixhobo ezininzi zePCR zexesha lokwenyani

Isicelo seKit

Uhlalutyo lwe-qPCR

Ukuhamba komsebenzi

Umzobo

Ukugcinwa kunye nobomi beshelufu

Le khithi kufuneka igcinwe kude nokukhanya kwaye kufuneka igcinwe ku -20 ℃.Ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ ixesha elifutshane (iintsuku ezili-10).

Ukusungula izinto ezintsha, umgangatho olungileyo kunye nokuthembeka zizinto ezixabisekileyo ezingundoqo zeshishini lethu.Le migaqo namhlanje eyongezelelweyo kunanini na ngaphambili yenza isiseko sempumelelo yethu njengombutho wamazwe ngamazwe ophakathi komgangatho oPhezulu oPhezulu oPhezulu oLungileyo ngexesha lokwenyani iPCR Kit Hotstart Taq DNA Polymerase Power Green Qpcr Mix , Inkampani yethu izinikele ekuboneleleni abathengi ngeemveliso ezikumgangatho ophezulu nezizinzileyo ngexabiso elikhuphisanayo, ukwenza wonke umxhasi aneliseke ngeemveliso kunye neenkonzo zethu.
Umgangatho olungileyoI-China Taq DNA Polymerase kunye ne-Qpcr, Xa unomdla kuyo nayiphi na into yethu elandelayo ukujonga uluhlu lwemveliso yethu, nceda uzive ukhululekile ukuqhagamshelana nathi ngemibuzo.Uya kukwazi ukusithumelela ii-imeyile kwaye uqhagamshelane nathi ukuze sibonisene kwaye siya kukuphendula ngokukhawuleza ukuba sikwazi ukwenza njalo.Ukuba kufanelekile, ungafumana idilesi yethu kwiwebhusayithi yethu kwaye uze kwishishini lethu.okanye ulwazi olongezelelweyo lwezinto zethu wedwa.Sisoloko sikulungele ukwakha ubudlelwane bentsebenziswano obude nobuzinzileyo nabo nabaphi na abathengi abanokubakho kwiindawo ezinxulumeneyo.