Inokuba yindlela entle yokuphucula izisombululo kunye nenkonzo yethu.Injongo yethu iya kuba kukwakha iimveliso zobuchule kubathengi abanamava aphezulu okusebenza kuMgangatho oLungileyo wePlasmid DNA Mini yokuCoca iKit, Ngokuqhelekileyo sithatha itekhnoloji kunye nabathengi njengezona ziphezulu.Siqhele ukwenza umsebenzi wenziwe nzima ukuyila amaxabiso amahle kubathengi bethu kwaye sinike abathengi bethu iimveliso kunye neenkonzo ezingcono.
Inokuba yindlela entle yokuphucula izisombululo kunye nenkonzo yethu.Injongo yethu iya kuba kukwakha iimveliso zobuchule kubathengi abanamava okusebenza aphezuluUkuhlanjululwa kwe-asidi ye-nucleic yaseTshayina kunye nePlasmid DNA yokuCoca, Sigxile ekuboneleleni ngenkonzo kubaxumi bethu njengento ephambili ekomelezeni ubudlelwane bethu bexesha elide.Ubukho bethu obuqhubekayo bezinto ezithengiweyo zodidi oluphezulu ngokudityaniswa nenkonzo yethu ebalaseleyo yokuthengisa kwangaphambili kunye nasemva kokuthengisa kuqinisekisa ukhuphiswano olomeleleyo kwimakethi ekhulayo yehlabathi.Sizimisele ukusebenzisana nabahlobo bezoshishino abasuka ekhaya naphesheya kunye nokudala ikamva elihle kunye.

Iinkcazelo Kit

I-FOREGENE'S nucleic acid extraction kunye neemveliso zechungechunge zokucoca zinokukhutshwa kwaye zihlanjululwe kwizinga eliphezulu le-nucleic acids ezivela kwimithombo eyahlukeneyo kunye neesampuli zamacandelo anzima.Iimveliso ze-DNA extraction series zisekelwe kwi-DNA-Kuphela i-silica membrane ye-silica iikholamu zokuhlanjululwa kunye ne-reagents ye-formula eyodwa enokuthi ihlambulule i-DNA ephezulu kwiisampuli ezahlukeneyo.

I-General Plasmid Mini Kit isebenzisa iteknoloji yekholomu yokucoca i-DNA-Kuphela kunye nefomula ye-SDS ye-lysis esebenzayo yokufumana i-plasmid DNA ephezulu kwiibhaktheriya ngaphakathi kwemizuzu engama-20.I-DNA-Kuphela i-silica gel membrane esetyenziswe kwikholamu ye-spin yinto entsha ekhethekileyo ye-FOREGENE, enokuthi ibhengeze i-DNA ngokufanelekileyo kwaye ngokuthe ngqo, kwaye isuse i-RNA, iiprotheni ezingcolileyo, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba elikhulu.Umthamo omkhulu wokubopha i-plasmid DNA yekholomu yokucoca kuphela yi-60μg DNA.Ubungakanani be-plasmid DNA ekhutshwe ngokwenene ihambelana nomthamo wesisombululo sebhaktheriya, inani leekopi ze-plasmid (ikopi ephezulu, ikopi ephantsi), ubunzima, iimeko zenkcubeko kunye nezinye izinto.

Iinkcukacha

Iimpawu&izinto eziluncedo

- Akukho ngcoliseko ye-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-plasmid DNA ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuphepha ukungcoliseka kwebhubhoratri nge-RNase yangaphandle.

- Isantya esikhawulezayo: I-Plasmid DNA extraction ingagqitywa kwimizuzu engama-20.

- Ikhuselekile: Akukho kutsalwa kwe-organic reagent efunekayo.

- Umgangatho ophezulu: I-plasmid efunyenwe ngokutsalwa ayinayo i-RNA, ayikho i-RNase kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

Isicelo seKit

I-Plasmid DNA extraction kunye nokuhlanjululwa kweebhaktheriya ze-Gram-negative.

Ukuhamba komsebenzi

Umzobo

Ukugcinwa kunye nobomi beshelufu

Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.

Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yifudumeze kwibhafu yamanzi engama-37 ℃ imizuzu eyi-10 ukunyibilikisa imvula, kwaye uyixube phambi kokusetyenziswa.

Inokuba yindlela entle yokuphucula izisombululo kunye nenkonzo yethu.Injongo yethu iya kuba kukwakha iimveliso zobuchule kubathengi abanamava aphezulu okusebenza kuMgangatho oLungileyo wePlasmid DNA Mini yokuCoca iKit, Ngokuqhelekileyo sithatha itekhnoloji kunye nabathengi njengezona ziphezulu.Siqhele ukwenza umsebenzi wenziwe nzima ukuyila amaxabiso amahle kubathengi bethu kwaye sinike abathengi bethu iimveliso kunye neenkonzo ezingcono.
Umgangatho olungileyoUkuhlanjululwa kwe-asidi ye-nucleic yaseTshayina kunye nePlasmid DNA yokuCoca, Sigxile ekuboneleleni ngenkonzo kubaxumi bethu njengento ephambili ekomelezeni ubudlelwane bethu bexesha elide.Ubukho bethu obuqhubekayo bezinto ezithengiweyo zodidi oluphezulu ngokudityaniswa nenkonzo yethu ebalaseleyo yokuthengisa kwangaphambili kunye nasemva kokuthengisa kuqinisekisa ukhuphiswano olomeleleyo kwimakethi ekhulayo yehlabathi.Sizimisele ukusebenzisana nabahlobo bezoshishino abasuka ekhaya naphesheya kunye nokudala ikamva elihle kunye.