I-China iMgangatho oPhakamileyo waseTshayina woLungiselelo lweSampuli ye-PCR ye-Automatic Nucleic Acid yokucoca uMenzi kunye noMboneleli |I-Foregene

Umgangatho oPhakamileyo we-PCR yeSampulu yokuLungiselela iSampuli yokuLungiselela i-Automatic Nucleic Acid Purification

Umgangatho oPhakamileyo we-PCR yeSampuli yokuLungiselela iSampuli yokuLungiselela i-Automatic Nucleic Acid yokucoca i-Extractor Featured Image

Inkcazelo yeKit:

Le khithi isebenzisa itekhnoloji ye-RT PCR yexesha lokwenyani (rRT-PCR) ukufunyaniswa komgangatho we-SARS-CoV-2 (i-ORF1ab gene kunye ne-N gene) ye-nucleic acids kwiisampulu ze-nasopharyngeal okanye ze-oropharyngeal swab, nge-multiplex PCR fluorescent probe method. ukufunyanwa ngokukhawuleza okukhulu.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukufumana ukuzaliseka komthengi yinjongo yenkampani yethu ngaphandle kwesiphelo.Siza kwenza amalinge amabi okufumana izisombululo ezitsha nezikumgangatho ophezulu, sidibane neenkcukacha zakho ezikhethekileyo kwaye sikubonelele ngokuthengiswa kwangaphambili, ukuthengiswa kunye nababoneleli emva kokuthengiswa koMgangatho oPhakamileyo we-PCR weSampuli yokuLungiselela i-Automatic Nucleic Acid Purification Extractor.
Ukufumana ukuzaliseka komthengi yinjongo yenkampani yethu ngaphandle kwesiphelo.Siza kwenza amalinge amabi okufumana izisombululo ezitsha nezikumgangatho ophezulu, sidibane neenkcukacha zakho ezizodwa kwaye sikubonelele ngokuthengisa kwangaphambili, ukuthengisa kunye nababoneleli emva kokuthengisa.IThemomitha yeInfrared yaseTshayina, Isixhobo seNzululwazi yoBomi, ngoku sineminyaka eyi-8 yamava emveliso kunye neminyaka emi-5 yamava ekurhwebeni kunye nabathengi kwihlabathi liphela.abathengi bethu basasazwa ikakhulu kuMntla Melika, eAfrika naseMpuma Yurophu.sinokubonelela ngeemveliso ezikumgangatho ophezulu ngexabiso elikhuphisana kakhulu.

Iinkcukacha

48 Rxns, 96 Rxns

Hayi.	Icandelo	Isixa				Amacandelo aphambili
Hayi.	Icandelo	48Rxns		96 Rxns		Amacandelo aphambili
1	I-agent yokukhululwa kwe-Nucleic acid	1.4mL / ityhubhu	2 iityhubhu	5.3 mL/ibhotile	1 ibhotile	I-surfactant
2	Umkhuseli we-RNA	27 μL/ityhubhu	ityhubhu e-1	53 μL/ityhubhu	ityhubhu e-1	I-RNase inhibitor
3	Isisombululo sokusabela kwe-SARS-CoV-2	800 μL/ityhubhu	ityhubhu e-1	1600 μL/ityhubhu	ityhubhu e-1	Iprimer, probe, reaction buffer, dNTP
4	Umxube we-enzyme ye-SARS-CoV-2	80 μL/ityhubhu	ityhubhu e-1	160 μL/ityhubhu	ityhubhu e-1	Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV
5	Ulawulo olulungileyo lwe-SARS-CoV-2	100 μL/ityhubhu	ityhubhu e-1	100 μL/ityhubhu	ityhubhu e-1	I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA
6	Ulawulo olubi lwe-SARS-CoV-2	1200 μL/ityhubhu	ityhubhu e-1	1200 μL/ityhubhu	ityhubhu e-1	TE buffer

Iimpawu&izinto eziluncedo

Ngokukhawuleza kwaye kulula.
Gwema ilahleko ye-nucleic acid.
Umda wokubona okuphantsi kunye novakalelo oluphezulu.
Imfuneko yezixhobo eziphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu.

Ukuqonda ukuqonda kunokufikelela kwinqanaba eliphantsi njengeekopi ze-500 / mL).

GCE/mL	Ct ixabiso
GCE/mL	Njini	Uhlobo lweORF1ab
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N / A	N / A
2.00E-01	N / A	N / A
NC	N / A	N / A

Igophe lokukhulisa umgca

igophe lokukhulisa-RT-qPCR

Kusetyenziswa kwanjani

1. Isixhobo esisebenzayo

i-covid-19 ikiti yokubona i-nucleic acid1

2. Inkqubo yokufumanisa

i-covid-19 ikiti yokubona i-nucleic acid2

Isampulu yokusetyenzwa: Isampuli ye-Naopharyngeal okanye i-oropharyngeal swab (inyathelo eli-1 kuphela) (Linda imizuzu eyi-10)

PCR amplificaton(<=55min)

Umsebenzi:

i-covid-19 ikiti yokubona i-nucleic acid4

Ugcino

itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;

yokugcina
ugcino2

Beka ubomi kwishelufa:1 unyaka

Ukufumana ukuzaliseka komthengi yinjongo yenkampani yethu ngaphandle kwesiphelo.Siza kwenza amalinge amabi okufumana izisombululo ezitsha nezikumgangatho ophezulu, sidibane neenkcukacha zakho ezikhethekileyo kwaye sikubonelele ngokuthengiswa kwangaphambili, ukuthengiswa kunye nababoneleli emva kokuthengiswa koMgangatho oPhakamileyo we-PCR weSampuli yokuLungiselela i-Automatic Nucleic Acid Purification Extractor.
Umgangatho ophezulu weIThemomitha yeInfrared yaseTshayina, Isixhobo seNzululwazi yoBomi, ngoku sineminyaka eyi-8 yamava emveliso kunye neminyaka emi-5 yamava ekurhwebeni kunye nabathengi kwihlabathi liphela.abathengi bethu basasazwa ikakhulu kuMntla Melika, eAfrika naseMpuma Yurophu.sinokubonelela ngeemveliso ezikumgangatho ophezulu ngexabiso elikhuphisana kakhulu.

Ngaphambili: Nikeza ngoVavanyo lwe-Acid yaseChina/iKit yokuFumana i-Rna (iMultiple RT-PCR Fluorescence Probing (Lyophilised)

Okulandelayo: Umzi-mveliso waseTshayina weTechstar yokuLahla iiKits zeSampuli yokuKhupha iReagent ngaphandle kwe-Rna/ Nucleic Acid Extraction Kit Kit Reagent Kits ngexesha langempela

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