Enesimo sengqondo esilungileyo nesinenkqubela ekuthandeni kwabathengi, ishishini lethu lihlala liphucula into yethu ngokugqwesileyo ukwanelisa iminqweno yabathengi kwaye lijolise ngakumbi kukhuseleko, ukuthembeka, iimfuno zokusingqongileyo, kunye nokuyila koMvelisi we-China Magnetic Beads Nucleic Acid PCR Extraction Kit kunye ne-CE yePoct PCR Machine, Ngokunyanisekileyo sikhula kunye nethemba lokusingqongileyo.
Enesimo sengqondo esilungileyo nesiqhubela phambili ekuthandeni kwabathengi, ishishini lethu lihlala liphucula into yethu ngokugqwesileyo ukwanelisa iminqweno yabathengi kwaye lijolise ngakumbi kukhuseleko, ukuthembeka, iimfuno zokusingqongileyo, kunye nokusungula izinto ezintsha.China DNA Extraction Kit, Nucleic Acid Extraction Kit, Sithobela umxhasi woku-1, umgangatho ophezulu we-1, ukuphuculwa okuqhubekayo, inzuzo efanayo kunye nemigaqo yokuphumelela.Xa intsebenziswano kunye nomthengi, sihambisa abathengi ngeyona nkonzo iphezulu iphezulu.Ukuseka ubudlelwane obuhle beshishini sisebenzisa umthengi waseZimbabwe ngaphakathi kwishishini, sizenzele igama kunye nodumo.Kwangelo xesha linye, wamkele ngeentliziyo ezipheleleyo amathemba amatsha namadala kwinkampani yethu ukuya kuthethathethana namashishini amancinci.

Iinkcazelo

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo enokuthi ikhulule ngokukhawuleza i-RNA kwiisampulu zeseli ezikhuliswe kwiimpendulo ze-RT-qPCR, ngaloo ndlela iphelisa inkqubo yokucoca i-RNA ethatha ixesha kunye nenzima.Ithemplethi ye-RNA inokufumaneka kwimizuzu nje esi-7.Umxube we-RT oyi-5 × ngokuthe ngqo kunye ne-2 × Direct qPCR Mix-SYBR ii-reagents ezibonelelwe yikhithi zinokufumana ngokukhawuleza nangempumelelo iziphumo zePCR zexesha lokwenyani.

I-5 × Direct RT Mix kunye ne-2 × Direct qPCR Mix-SYBR inokunyamezela inhibitor eqinile, kunye ne-lysate yeesampuli zingasetyenziswa njenge template ye-RT-qPCR ngokuthe ngqo.Le khithi iqulethe i-RNA ephezulu ehambelana ne-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl₂, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.

Iinkcukacha

200×20μl Rxns, 1000×20μl Rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ilula kwaye isebenzayo : ngeteknoloji ye-Cell Direct RT, iisampuli ze-RNA zinokufumaneka kwimizuzu nje eyi-7.

∎ Imfuno yesampulu incinci, ingaphantsi njengeeseli ezili-10 zinokuvavanywa.

■ Ukugqithisa okuphezulu: inokubona ngokukhawuleza i-RNA kwiiseli ezikhuliswe kwii-384, 96, 24, 12, 6-well plates.

■ I-DNA Eraser inokususa ngokukhawuleza iigenomes ezikhutshiweyo, inciphise kakhulu impembelelo kwiziphumo zovavanyo ezilandelayo.

■ Inkqubo elungiselelwe i-RT kunye ne-qPCR yenza amanyathelo amabini e-RT-PCR akhutshelwe umva asebenze ngakumbi kwaye i-PCR icace ngakumbi, kwaye imelane ngakumbi ne-RT-qPCR reaction inhibitors.

Isicelo seKit

Ububanzi besicelo: iiseli ezikhuliswe.

- I-RNA ekhutshwe ngesampuli ye-lysis: isebenza kuphela kwi-template ye-RT-qPCR yale khithi.

- Ikiti ingasetyenziselwa ezi njongo zilandelayo: uhlalutyo lwe-gene expression, ukuqinisekiswa kwe-siRNA-mediated gene silence effect, ukuhlolwa kweziyobisi, njl.

Umzobo

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-4℃;Icandelo II kufuneka ligcinwe kwi -20 ℃.

I-Foregene Protease Plus II kufuneka igcinwe ku-4℃, ungakhenkcezisi ku -20℃.

I-Reagent 2 × Direct qPCR Mix-SYBR kufuneka igcinwe kwi -20 ℃ ebumnyameni;ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (ukusetyenziswa ngaphakathi kweentsuku ze-10) .Enesimo sengqondo esilungileyo kunye nenkqubela phambili ekuthandeni umthengi, ishishini lethu liphucula ngokuqhubekayo into yethu egqwesileyo ukwanelisa iminqweno yabathengi kwaye igxininise ngakumbi kukhuseleko, ukuthembeka, iimfuno zokusingqongileyo, kunye nokuveliswa kwe-Accle ye-PC ye-Accle ye-CER ye-PC ye-Nuclear Manufacturer kunye ne-PC ye-Nuclean Ndiyathemba ukuba siyakhula kunye namathemba ethu kuyo yonke indalo esingqongileyo.
Umenzi weChina DNA Extraction Kit, Nucleic Acid Extraction Kit, Sithobela umxhasi woku-1, umgangatho ophezulu we-1, ukuphuculwa okuqhubekayo, inzuzo efanayo kunye nemigaqo yokuphumelela.Xa intsebenziswano kunye nomthengi, sihambisa abathengi ngeyona nkonzo iphezulu iphezulu.Ukuseka ubudlelwane obuhle beshishini sisebenzisa umthengi waseZimbabwe ngaphakathi kwishishini, sizenzele igama kunye nodumo.Kwangelo xesha linye, wamkele ngeentliziyo ezipheleleyo amathemba amatsha namadala kwinkampani yethu ukuya kuthethathethana namashishini amancinci.