Uvavanyo lwe-asidi ye-nucleic yaseTshayina kunye negumbi lokusampula uMenzi kunye noMboneleli |I-Foregene

uvavanyo nucleic acid kunye negumbi isampuli

Inkcazelo yeKit:

Le khithi isebenzisa itekhnoloji ye-RT PCR yexesha lokwenyani (rRT-PCR) ukufunyaniswa komgangatho we-SARS-CoV-2 (i-ORF1ab gene kunye ne-N gene) ye-nucleic acids kwiisampulu ze-nasopharyngeal okanye ze-oropharyngeal swab, nge-multiplex PCR fluorescent probe method. ukufunyanwa ngokukhawuleza okukhulu.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Injongo yethu kufuneka ibe kukudibanisa kunye nokuphucula umgangatho ophezulu kunye nenkonzo yempahla esele ikhona, okwangoku sihlala siphuhlisa iimveliso ezintsha kunye nezisombululo zokuzalisekisa iimfuno zabathengi ezahlukeneyouvavanyo nucleic acid kunye negumbi isampuli, Siyathembisa ukuba siza kuzama okusemandleni ethu ukukuzisa ngomgangatho weprimiyamu kunye nezisombululo ezisebenzayo.
Injongo yethu kufuneka ibe kukudibanisa kunye nokuphucula umgangatho ophezulu kunye nenkonzo yempahla esele ikhona, okwangoku sihlala siphuhlisa iimveliso ezintsha kunye nezisombululo zokuzalisekisa iimfuno zabathengi ezahlukeneyouvavanyo nucleic acid kunye negumbi isampuli, Ngaphezulu kweminyaka eyi-9 yamava kunye neqela leengcali, ngoku sithumele izisombululo zethu kumazwe amaninzi nakwimimandla kwihlabathi liphela.Samkela abathengi, imibutho yamashishini kunye nabahlobo abavela kuzo zonke iindawo zehlabathi ukuba baqhagamshelane nathi kwaye bafune intsebenziswano ukuze bafumane inzuzo efanayo.

Iinkcukacha

48 Rxns, 96 Rxns

Hayi.	Icandelo	Isixa				Amacandelo aphambili
Hayi.	Icandelo	48Rxns		96 Rxns		Amacandelo aphambili
1	I-agent yokukhululwa kwe-Nucleic acid	1.4mL / ityhubhu	2 iityhubhu	5.3 mL/ibhotile	1 ibhotile	I-surfactant
2	Umkhuseli we-RNA	27 μL/ityhubhu	ityhubhu e-1	53 μL/ityhubhu	ityhubhu e-1	I-RNase inhibitor
3	Isisombululo sokusabela kwe-SARS-CoV-2	800 μL/ityhubhu	ityhubhu e-1	1600 μL/ityhubhu	ityhubhu e-1	Iprimer, probe, reaction buffer, dNTP
4	Umxube we-enzyme ye-SARS-CoV-2	80 μL/ityhubhu	ityhubhu e-1	160 μL/ityhubhu	ityhubhu e-1	Isiqalo esishushu se-Taq enzyme, i-enzyme ye-M-MLV
5	Ulawulo olulungileyo lwe-SARS-CoV-2	100 μL/ityhubhu	ityhubhu e-1	100 μL/ityhubhu	ityhubhu e-1	I-plasmid edibeneyo equlethe iqhekeza ekujoliswe kulo, i-RNA
6	Ulawulo olubi lwe-SARS-CoV-2	1200 μL/ityhubhu	ityhubhu e-1	1200 μL/ityhubhu	ityhubhu e-1	TE buffer

Iimpawu&izinto eziluncedo

Ngokukhawuleza kwaye kulula.
Gwema ilahleko ye-nucleic acid.
Umda wokubona okuphantsi kunye novakalelo oluphezulu.
Imfuneko yezixhobo eziphantsi kunye nokusetyenziswa okubhetyebhetye kakhulu.

Ukuqonda ukuqonda kunokufikelela kwinqanaba eliphantsi njengeekopi ze-500 / mL).

GCE/mL	Ct ixabiso
GCE/mL	Njini	Uhlobo lweORF1ab
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N / A	N / A
2.00E-01	N / A	N / A
NC	N / A	N / A

Igophe lokukhulisa umgca

igophe lokukhulisa-RT-qPCR

Kusetyenziswa kwanjani

1. Isixhobo esisebenzayo

i-covid-19 ikiti yokubona i-nucleic acid1

2. Inkqubo yokufumanisa

i-covid-19 ikiti yokubona i-nucleic acid2

Isampulu yokusetyenzwa: Isampuli ye-Naopharyngeal okanye i-oropharyngeal swab (inyathelo eli-1 kuphela) (Linda imizuzu eyi-10)

PCR amplificaton(<=55min)

Umsebenzi:

i-covid-19 ikiti yokubona i-nucleic acid4

Ugcino

itywinwe ekukhanyeni kwaye igcinwe kwi -20±5℃;

yokugcina
ugcino2

Beka ubomi kwishelufa:1 unyaka

Injongo yethu kufuneka ibe kukudibanisa kunye nokuphucula umgangatho ophezulu kunye nenkonzo yempahla esele ikhona, okwangoku sihlala siphuhlisa iimveliso ezintsha kunye nezisombululo zokuzalisekisa iimfuno zabathengi abahlukeneyo kwiFactory ebonelelwe yi-China FDA Sterile Medical Nylon Flock Flocked Sampling Specimen Collection Collection Umqala weNasopharyngeal Flocked Swabs Liquid Transport Medium, Siyathembisa ukuba uza kuzama ngeyona ndlela iphezulu yezothutho.
Ifektri ebonelelwe yi-China Swab, i-Injection Instrument, Ngaphezu kweminyaka engama-9 yamava kunye neqela leengcali, ngoku sithumele izisombululo zethu kumazwe amaninzi nakwimimandla kwihlabathi jikelele.Samkela abathengi, imibutho yamashishini kunye nabahlobo abavela kuzo zonke iindawo zehlabathi ukuba baqhagamshelane nathi kwaye bafune intsebenziswano ukuze bafumane inzuzo efanayo.

Ngaphambili: I-OEM/ODM Factory China Vtm Disposable Virus Sampling Kit Flocked Nylon Nesal Throat Swab

Okulandelayo: Isampulu yasimahla ye-China CE Nucleic Acid DNA/Rna Extraction Kit+Instrument

Bhala umyalezo wakho apha kwaye uwuthumele kuthi