Ubonelelo lwe-OEM China oludumileyo lweGenomic Nucleic Acid yokuCoca iMagnetic Viral Rna Isolation yeDNA Extraction Kit
Ngokuphathelele amaxabiso akhuphisanayo, siyakholelwa ukuba uyakube ukhangela kude nakude kuyo nantoni na enokusoyisa.Singatsho ngokuqiniseka ngokupheleleyo ukuba kulo mgangatho kumaxabiso alolo hlobo sisezantsi kwi-OEM Supply China i-Genomic Nucleic Acid yokucoca iMagnetic Viral Rna Isolation DNA Extraction Kit, Umgangatho ophezulu kunye namazinga okhuphiswano enza izinto zethu zonwabe kukuma okuphezulu kuyo yonke indawo kwigama.
Ngokuphathelele amaxabiso akhuphisanayo, siyakholelwa ukuba uyakube ukhangela kude nakude kuyo nantoni na enokusoyisa.Singatsho ngokuqinisekileyo ukuba kumgangatho onjalo kumaxabiso alolo hlobo singabona basezantsi kuwoChina PCR, Nucleic Acid Test Kit, Uphuhliso lwenkampani yethu alufuni kuphela isiqinisekiso somgangatho, ixabiso elifanelekileyo kunye nenkonzo egqibeleleyo, kodwa kuxhomekeke ekuthembekeni kunye nenkxaso yomthengi wethu!Kwixesha elizayo, siya kuqhubeka ngeyona nkonzo inamava kunye nomgangatho ophezulu wokubonelela ngelona xabiso likhuphisanayo, Kunye nabathengi bethu kwaye sifezekise i-win-win!Wamkelekile kumbuzo kunye nokubonisana!
Inkcazo
Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.
Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.
IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.
Amacandelo ekhithi
Ukwakhiwa kweKit | RE-03111 | RE-03114 |
50 T | 200 T | |
Isithinteli cRL1* | 25 ml | 100 ml |
Isithinteli cRL2 | 15 ml | 60 ml |
Isithinteli RW1* | 25 ml | 100 ml |
Isithinteli RW2 | 24 ml | 96 ml |
RNase-Free ddH2O | 10 ml | 40 ml |
Ikholamu ye-RNA Kuphela | 50 | 200 |
Ikholamu yokucoca iDNA | 50 | 200 |
Umyalelo | 1 | 1 |
*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.
Iimpawu&izinto eziluncedo
■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.
Isicelo seKit
Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.
Ukuhamba komsebenzi
Umzobo
Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.
Ukugcinwa kunye nobomi beshelufu
Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).
I-Buffer cRL1 ingagcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol(ukhetho). Malunga namaxabiso akhuphisanayo, siyakholelwa ukuba uya kukhangela kude nakude kuyo nayiphi na into enokusibetha.Singatsho ngokuqiniseka ngokupheleleyo ukuba kulo mgangatho kumaxabiso alolo hlobo sisezantsi kwi-OEM Supply China i-Genomic Nucleic Acid yokucoca iMagnetic Viral Rna Isolation DNA Extraction Kit, Umgangatho ophezulu kunye namazinga okhuphiswano enza izinto zethu zonwabe kukuma okuphezulu kuyo yonke indawo kwigama.
Ubonelelo lweOEMChina PCR, Nucleic Acid Test Kit, Uphuhliso lwenkampani yethu alufuni kuphela isiqinisekiso somgangatho, ixabiso elifanelekileyo kunye nenkonzo egqibeleleyo, kodwa kuxhomekeke ekuthembekeni kunye nenkxaso yomthengi wethu!Kwixesha elizayo, siya kuqhubeka ngeyona nkonzo inamava kunye nomgangatho ophezulu wokubonelela ngelona xabiso likhuphisanayo, Kunye nabathengi bethu kwaye sifezekise i-win-win!Wamkelekile kumbuzo kunye nokubonisana!
I-RNA ayikhutshwa okanye izivuno ze-RNA ziphantsi
Kuhlala kukho izinto ezahlukeneyo ezichaphazela ukusebenza kakuhle kokubuyisela, njengale: umxholo we-RNA we-tissue, indlela yokusebenza, umthamo we-lution, njl.
1. Ibhafu ye-ice okanye i-cryogenic (4 ° C) i-centrifugation yenziwa ngexesha lokusebenza.
Isincomo: Sebenza kwiqondo lokushisa (15-25 ° C) kuyo yonke inkqubo, musa ukuhlamba i-ice kunye ne-centrifuge kumaqondo aphantsi.
2. Ukugcinwa kwesampulu engafanelekanga okanye ixesha lokugcina isampuli.
Isindululo: Gcina iisampulu kwi -80 °C okanye umkhenkce kwinitrogen engamanzi kwaye uthintele ukusetyenziswa okuphindaphindiweyo komkhenkce;zama ukusebenzisa izicubu ezitsha okanye iiseli ezikhuliswayo zokutsalwa kwe-RNA.
3. I-lysis yesampuli eyaneleyo.
Isincomo: Xa i-homogenizing tissue, qinisekisa ukuba i-tissue i-homogenized ngokwaneleyo kwaye iiseli zethishu zahlulwe ngokwaneleyo ukucacisa ukukhululwa kwe-RNA.
4. I-eluent ayidityaniswa ngokuchanekileyo.
Ingcebiso: Qinisekisa ukuba i-RNase-Free ddH2I-O yongezwa kwi-dropwise phakathi kwe-membrane yekholomu yokucoca.
5. Umthamo ochanekileyo we-absolute ethanol awuzange wongezwe kwi-Buffer RL2 okanye kwi-Buffer RW2.
Ingcebiso: Landela imiyalelo, yongeza umthamo ochanekileyo we-ethanol epheleleyo kwi-Buffer RL2 kunye ne-Buffer RW2 kwaye udibanise kakuhle phambi kokusebenzisa ikhithi.
6. Idosi yesampula yezicubu ayifanelekanga.
Isincomo: Sebenzisa i-10-20 mg yezicubu okanye (1-5) × 106iiseli nge-500 μl buffer RL1, njengoko ukusetyenziswa kwethishu ngokugqithisileyo kunokubangela ukutsalwa kwe-RNA okucuthiweyo.
7. Umthamo ongafanelekanga okanye i-elution engaphelelanga.
Isincomo: Umthamo we-elution wekholamu yokucoca yi-50-200 μl;ukuba isiphumo se-elution asanelisi, kuyacetyiswa ukuba kwandiswe ixesha lokubeka ubushushu begumbi emva kokongeza i-RNase-Free ddH efudunyeziweyo.2O, umz. kwi-5-10 min.
8.Ikholamu yokucoca inentsalela ye-ethanol emva kokuhlamba kwe-Buffer RW2.
Isincomo: Ukuba kukho intsalela ye-ethanol emva kokuhlamba kwe-Buffer RW2, i-tube engenanto ye-centrifugation ye-1min, ixesha le-tube engenanto yokusebenza kwe-centrifugation linganyuswa ukuya kwi-2min, okanye ikholomu yokucoca inokubekwa kwindawo yokushisa ye-5 min ukususa ngokwaneleyo i-ethanol eseleyo.
I-RNA ecocekileyo ithotywa
Umgangatho we-RNA ecocekileyo inxulumene nezinto ezifana nokugcinwa kwesampulu, ukungcoliswa kwe-RNase, kunye nokukhwabanisa, njl.
1. Iisampulu zezicubu azigcinwanga ngexesha.
Isincomo: Ukuba iisampulu zethishu okanye iiseli azisetyenziswanga ngexesha elifanelekileyo emva kokuqokelelwa, ngoko nangoko gcina i-cryopreserve kwi -80 °C okanye initrogen engamanzi.Ukukhupha i-RNA, sebenzisa ithishu esanda kuthathwa okanye isampulu yeseli xa kunokwenzeka.
2. Ukunyibilika okuphindaphindiweyo kweesampuli zethishu.
Isincomo: Xa ugcina iisampulu zethishu, kungcono ukuba uzinqumle zibe ngamaqhekeza amancinci ukuze zigcinwe, kwaye ususe esinye seziqwenga xa uzisebenzisa ukuphepha ukunyibilika okuphindaphindiweyo kwesampulu kunye nokuthotywa kwe-RNA.
3. I-RNase yaziswa okanye ayinxibi iiglavu ezilahlwayo, iimaski, njl njl ngexesha lokusebenza.
Isincomo: Imifuniselo yokutsalwa kwe-RNA yenziwa ngcono kumagumbi okukhohlisa e-RNA kwaye itafile iyacinywa phambi kovavanyo.
Nxiba iiglavu ezilahlwayo kunye nemaski ngexesha lovavanyo lokunciphisa ukuthotywa kwe-RNA okubangelwa kukwaziswa kwe-RNase.
4. Ii-reagents zingcolisekile nge-RNase ngexesha lokusetyenziswa.
Ingcebiso: Faka endaweni entsha yeSilwanyana esipheleleyo se-RNA ye-Isolation Kit ukulungiselela imifuniselo ehambelanayo.
5. Iityhubhu ze-centrifuge, iingcebiso, njl.njl ezisetyenziswa kwi-RNA manipulation zingcolile nge-RNase.
Ingcebiso: Qinisekisa ukuba iityhubhu ze-centrifuge, iingcebiso, iipipette, njl.njl ezisetyenziswa kwi-RNA extraction azinayo yonke i-RNase.
I-RNA esulungekileyo ichaphazela imifuniselo esezantsi
I-RNA ihlanjululwe yikholomu yokucoca, ukuba iiyoni zetyuwa, umxholo weprotheyini mkhulu kakhulu uya kuchaphazela umfuniselo osezantsi, onje: uguqulelo lombhalo, Northern Blot et al.
1. I-RNA ekhutshiweyo ineentsalela ze-ion yetyuwa.
Isincomo: Qinisekisa ukuba umthamo ochanekileyo we-ethanol wongezwe kwi-Buffer RW2 kwaye wenze i-2 ikholamu yokuhlanjululwa kwi-speed ye-centrifugal eboniswe ekusebenzeni;ukuba kukho nayiphi na intsalela ye-ion yetyuwa, shiya ikholomu yokucoca kwi-Buffer RW2 ye-5 min kwiqondo lokushisa elingaphakathi kwaye wenze i-centrifugation ukwandisa ukukhutshwa kwetyuwa.
2. Intsalela ye-Ethanol kwi-RNA elutioned.
Isincomo: Qinisekisa ukuba emva kokuhlamba i-buffer RW2, yenza i-tube engenanto ye-centrifugation operation kwisantya se-centrifugation esibonakaliswe ukusebenza, ukwandisa ixesha le-tube engenanto ye-centrifugation operation ukuya kwi-2 min ukuba kusekho i-ethanol intsalela, okanye uyishiye kwindawo yokushisa kwe-5 m.