Umgangatho ophezulu othembekileyo kunye nokuma kwetyala okumangalisayo yimigaqo yethu, eya kusinceda kwindawo ephezulu.Ukubambelela kumgaqo wakho "womgangatho wokuqala, umxhasi ophakamileyo" kwi-Intanethi ye-Intanethi yokutsalwa kwe-Viru ye-Magnetic Beads Indlela ye-Viral DNA / i-Rna Nucleic Acid Extraction Kits ye-Real Time yokuFunyaniswa kwe-PCR nge-CE, Ngelixa usebenzisa uphuculo loluntu kunye noqoqosho, ishishini lethu liya kubamba i-tenet "Gxila kwintembeko, umgangatho ophezulu, sibala yonke into yokuqala kunye nomthengi ngamnye".
Umgangatho ophezulu othembekileyo kunye nokuma kwetyala okumangalisayo yimigaqo yethu, eya kusinceda kwindawo ephezulu.Ukubambelela kumgaqo wakho "womgangatho kuqala, umxhasi ophakamileyo" weI-DNA yeGazi yaseTshayina kunye neKhiti yokuKhupha, Inkampani yethu igxininisa kwinjongo "yokuthatha inkonzo ephambili kumgangatho, isiqinisekiso somgangatho wentengiso, yenza ishishini ngokholo oluhle, ukunikezela ngenkonzo efanelekileyo, ngokukhawuleza, echanekileyo kunye nexesha elifanelekileyo kuwe".Samkela abathengi abadala nabatsha ukuba bathethathethane nathi.Siza kukukhonza ngako konke ukunyaniseka!

Iinkcukacha

200 ×20μl rxns, 2000 ×20μl rxns 2× SuperEasy^TMI-Mix-EDTA: Iqulethe i-D-Taq DNA Polymerase elungiswe ngokukodwa, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR reaction enhancer, i-optimizer kunye ne-stabilizer, njl njl. Kwi-reaction ye-PCR, yongeza nje igazi elichanekileyo elichasene ne-anticoagulated lonke, iiprimers, kunye ne-ddH.₂O ukuya kwi-2 × SuperEasy ehambelanayo^TMI-Mix-EDTA iza kusetyenziswa kwi-PCR reaction.

I-6 × i-DNA yokuLayisha i-Buffer: I-Buffer yokuLayisha ayinayo i-SDS.Kunconywa ukusebenzisa i-6 × DNA Loading Buffer efakwe kunye nekiti xa wenza i-agarose gel electrophoresis ukuze ufumane iziphumo ezilungileyo ze-electrophoresis.Musa ukusebenzisa i-Loading Buffer equkethe i-SDS, ngaphandle koko kuya kubakho umsila omkhulu wokukhanya okukhanyayo kwindlela ngexesha le-electrophoresis, eya kuchaphazela iziphumo zovavanyo.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

-Akukho mfuneko yokuchitha ixesha kunye nokuhlanjululwa kwe-DNA ebiza kakhulu, akukho unyango lwangaphambili, kunye nokusetyenziswa ngokuthe ngqo kwegazi elipheleleyo njenge-template ye-PCR.-Inkqubo inamandla okukhulisa amandla kwaye inokubona ngononophelo i-genome kunye namaqhekeza e-DNA ekujoliswe kuwo angaphandle egazini.-2 × SuperEasy^TMI-Mix-EDTA Ukunyamezela kwegazi eliphezulu: ukuya kwi-45% kwigazi lomntu kunye ne-20% kwigazi le-mouse.-Isampuli ivalwe ngokupheleleyo, ngoko akukho mfuneko yakukhathazeka malunga nongcoliseko lwesampulu kunye neziphumo zobuxoki ze-PCR.

Isicelo seKit

Kunikezelwe ukuchongwa ngqo kwePCR ye-EDTA anticoagulated lonke igazi.(Kubandakanya: igazi lomntu, igazi lempuku, igazi lenkukhu, igazi lentaka, igazi lenkomo, igazi lenja, njl.)

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Le khithi kufuneka igcinwe kwi -20°C.2 × SuperEasy^TMI-Mix-EDTA, ukuba isetyenziswe rhoqo, ingagcinwa kwi-4 ℃ ukwenzela ukugcinwa kwexesha elifutshane (ukusebenzisa kwiintsuku ze-10).6 × DNA Loading Buffer，Inokugcinwa kwi-4 ° C okanye -20 ° C ixesha elide.Umgangatho ophezulu kunye nokuma kwetyala okumangalisayo yimigaqo yethu, eya kusinceda kwindawo ephezulu.Ukubambelela kumgaqo wakho "womgangatho wokuqala, umxhasi ophakamileyo" kwi-Intanethi ye-Intanethi yokutsalwa kwe-Viru ye-Magnetic Beads Indlela ye-Viral DNA / i-Rna Nucleic Acid Extraction Kits ye-Real Time yokuFunyaniswa kwe-PCR nge-CE, Ngelixa usebenzisa uphuculo loluntu kunye noqoqosho, ishishini lethu liya kubamba i-tenet "Gxila kwintembeko, umgangatho ophezulu, sibala yonke into yokuqala kunye nomthengi ngamnye".
Umthumeli ngaphandle kwe-IntanethiI-DNA yeGazi yaseTshayina kunye neKhiti yokuKhupha, Inkampani yethu igxininisa kwinjongo "yokuthatha inkonzo ephambili kumgangatho, isiqinisekiso somgangatho wentengiso, yenza ishishini ngokholo oluhle, ukunikezela ngenkonzo efanelekileyo, ngokukhawuleza, echanekileyo kunye nexesha elifanelekileyo kuwe".Samkela abathengi abadala nabatsha ukuba bathethathethane nathi.Siza kukukhonza ngako konke ukunyaniseka!