Umgangatho wokuQala, kunye noMthengi oPhakamileyo sisikhokelo sethu sokubonelela ngeyona nkonzo ilungileyo kubathengi bethu.Namhlanje, sizama konke okusemandleni ethu ukuba ngomnye wabathengisi bangaphandle abagqwesileyo kwintsimi yethu ukuhlangabezana neemfuno ezingakumbi zoYilo oludumileyo lweChina 96t Automatic DNA Rna.Ukwahlulwa kwe-Nucleic AcidIsisombululo nge-CE kunye ne-FDA, Samkela ithemba lokwenza amashishini amancinci kunye nawe kwaye sinethemba lokonwaba ekuncamatheleni iinkcukacha ezithe vetshe zentengiso yethu.
Umgangatho Wokuqala, kwaye uMthengi oPhakamileyo sisikhokelo sethu sokubonelela ngeyona nkonzo ilungileyo kubathengi bethu.Namhlanje, sizama konke okusemandleni ethu ukuba ngomnye wabathengisi abagqwesileyo kwintsimi yethu ukuhlangabezana neemfuno zabathengi.China Nucleic Acid Isolution Isolation, Ukwahlulwa kwe-Nucleic Acid, Kuye nabani na onomdla kuyo nayiphi na into yethu kanye emva kokujonga uluhlu lwemveliso yethu, ngokuqinisekileyo kufuneka uzive ukhululekile ngokupheleleyo ukunxibelelana nathi malunga nemibuzo.Uyakwazi ukusithumelela ii-imeyile kwaye uqhagamshelane nathi ngokubonisana kwaye siya kukuphendula ngokukhawuleza njengoko sinakho.Ukuba kulula, ungakhangela idilesi yethu kwiwebhusayithi yethu kwaye uze kwishishini lethu ukuze ufumane ulwazi oluthe kratya lwentengiso yethu ngokwakho.Sisoloko sikulungele ukwakha ubudlelwane bentsebenziswano obandisiweyo kunye nezizinzileyo kunye nabo nabaphi na abathengi abanokubakho kwiinkalo ezinxulumeneyo.

Iinkcukacha

I-50 Preps, yokucoca i-DNA ye-genomic kwigazi elipheleleyo usebenzisa i-<1ml yegazi

I-Buffer DC: Ikhupha iiseli ezibomvu zegazi egazini.

Isithinteli BL1: Nikezela ngemeko yokwetyisa i-enzymatic.

Foregene Protease Plus: Iisampulu zegazi zokwetyiswa ngokweenzymatic kwindawo yeBuffer BL1.

I-Buffer BL2: Yenze i-Foregene Protease Plus kwaye ibonelele ngendawo yokulayisha i-DNA.

I-Buffer PW: Susa ukungcola okufana neprotheni kunye ne-RNA kwi-DNA.

I-Buffer WB1: Susa iiyoni zetyuwa ezishiyekileyo kwi-DNA.

I-Buffer EB: I-Elute i-DNA kwi-membrane yekholomu yokucoca.

Ikholamu ye-DNA kuphela: I-adsorbs i-DNA ye-genomic kwi-lysate.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.

-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kune-proteases efanayo, kwaye igaya iisampulu zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-40.

-Uncedo: I-centrifugation yenziwa kwiqondo lokushisa, i-4 ° C i-centrifugation okanye i-ethanol precipitation ye-DNA ayifuni.

-Ukhuseleko: Akukho kukhutshwa kwezinto eziphilayo ezisetyenzisiweyo.

-Umgangatho ophezulu: Iziqwenga ze-DNA ze-genomic ezikhutshwe zikhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

Isicelo seKit

Ikulungele ukutsalwa kunye nokucocwa kwe-DNA ye-genomic kwigazi elitsha okanye elinomkhenkce elichasene ne-anticoagulated.

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.

Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.

Isisombululo se-Foregene Protease Plus sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);iya kusebenza ngakumbi kwaye izinzile xa igcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuyigcina kwi-4 ° C, khumbula ukuba ungayigcini -20 ° C. Umgangatho wokuqala, kwaye uMthengi oPhakamileyo sisikhokelo sethu sokubonelela ngeyona nkonzo ingcono kubathengi bethu.Namhlanje, sizama konke okusemandleni ethu ukuba sibe ngomnye wabathengisi abagqwesileyo kwintsimi yethu ukuhlangabezana nabathengi imfuneko engaphezulu ye-96na Automatic DesignUkwahlulwa kwe-Nucleic AcidIsisombululo nge-CE kunye ne-FDA, Samkela ithemba lokwenza amashishini amancinci kunye nawe kwaye sinethemba lokonwaba ekuncamatheleni iinkcukacha ezithe vetshe zentengiso yethu.
Uyilo oludumileyo lweChina Nucleic Acid Isolution Isolation, I-Nucleic Acid Isolation, kuye nabani na onomdla kuyo nayiphi na into yethu kanye emva kokujonga uluhlu lwemveliso yethu, kufuneka uzive ukhululekile ngokupheleleyo ukunxibelelana nathi xa unemibuzo.Uyakwazi ukusithumelela ii-imeyile kwaye uqhagamshelane nathi ngokubonisana kwaye siya kukuphendula ngokukhawuleza njengoko sinakho.Ukuba kulula, ungakhangela idilesi yethu kwiwebhusayithi yethu kwaye uze kwishishini lethu ukuze ufumane ulwazi oluthe kratya lwentengiso yethu ngokwakho.Sisoloko sikulungele ukwakha ubudlelwane bentsebenziswano obandisiweyo kunye nezizinzileyo kunye nabo nabaphi na abathengi abanokubakho kwiinkalo ezinxulumeneyo.