Ukudala ixabiso elithe chatha kubathengi yintanda-bulumko yenkampani yethu;Ukukhula komthengi kukuleqa iPhepha leXabiso leNtsha yeNtsholongwane yeNtsholongwane eNtsha engaBandeli ye-Viral DNA / i-Rna Nucleic Acid Extraction Kit Kit Reagent Kits yeXesha lokwenyani iPCR yegazi, Samkela ngeentliziyo ezipheleleyo abathengi kwihlabathi liphela beze kwindawo yethu yokuvelisa kwaye babe nentsebenziswano yokuphumelela kunye nathi!
Ukudala ixabiso elithe chatha kubathengi yintanda-bulumko yenkampani yethu;ukukhula komthengi kukuleqa kwethu ukusebenzaI-DNA yeGazi yaseTshayina kunye neKhiti yokuKhupha, Sithobela umxhasi woku-1, umgangatho ophezulu we-1, ukuphuculwa okuqhubekayo, inzuzo efanayo kunye nemigaqo yokuphumelela.Xa intsebenziswano kunye nomthengi, sihambisa abathengi ngeyona nkonzo iphezulu iphezulu.Ukuseka ubudlelwane obuhle beshishini sisebenzisa umthengi waseZimbabwe ngaphakathi kwishishini, sizenzele igama kunye nodumo.Kwangelo xesha linye, wamkele ngeentliziyo ezipheleleyo amathemba amatsha namadala kwinkampani yethu ukuya kuthethathethana namashishini amancinci.

Iinkcazelo Kit

Le khithi ithatha i-Foregene Protease Plus entsha kunye ne-BL1 kunye ne-BL2 ye-buffer systems, ekwazi ukwetyisa ngokupheleleyo iisampuli zegazi ze-anticoagulant ngexesha elifutshane, ngaloo ndlela inqanda ukuthotywa kwe-DNA ukuya kwinqanaba eliphezulu kunye nokufumana ubuninzi be-DNA ye-genomic.Inkqubo yokucubungula igazi ngokukhawuleza kunye ne-DNA-Only column operation lula kakhulu ukutsalwa kwe-genome yegazi, okwenza i-DNA ye-genomic inokufumaneka kumgangatho ophezulu kunye nokucoceka okuphezulu kwimizuzu engama-40.

I-DNA-kuphela kwe-silica gel membrane esetyenziswe kwikholamu ye-centrifuge yale kit luhlobo olutsha lwezinto ezibonakalayo kwinkampani yethu, enokuthi ibophe i-DNA ngokufanelekileyo kwaye ngokuthe ngqo, kwaye inokususa i-RNA, ukungcola, iiprotheni, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba eliphezulu.Iziqwenga ze-DNA ezifunyenweyo zikhulu, zicocekile, zizinzile kwaye zithembekile umgangatho.Ubuninzi bokuthwala umthamo we-DNA-Only column yi-80 μg DNA.I-DNA efunyenweyo ingasetyenziselwa kwiimvavanyo zebhayoloji ye-molekyuli ezifana ne-enzyme digestion, i-PCR, i-hybridization yaseMzantsi kunye nokwakhiwa kwethala leencwadi.

Ikiti inokuqhubela phambili ukuya kwi-1ml yegazi ngexesha lokufumana inani elaneleyo le-DNA ye-genomic ephezulu.

Iinkcukacha

*: I-Buffer BL2 kunye ne-Buffer PW ziqulathe ityuwa elulwelo olucaphukisayo.Nceda unxibe iiglavu kwaye uthathe amanyathelo okhuseleko afanelekileyo xa usebenza.

Iimpawu&izinto eziluncedo

-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.

-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kune-proteases efanayo, kwaye igaya iisampulu zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-40.

-Uncedo: I-centrifugation yenziwa kwiqondo lokushisa, i-4 ° C i-centrifugation okanye i-ethanol precipitation ye-DNA ayifuni.

-Ukhuseleko: Akukho kukhutshwa kwezinto eziphilayo ezisetyenzisiweyo.

-Umgangatho ophezulu: Iziqwenga ze-DNA ze-genomic ezikhutshwe zikhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.

Isicelo seKit

Ikulungele ukutsalwa kunye nokucocwa kwe-DNA ye-genomic kwigazi elitsha okanye elinomkhenkce elichasene ne-anticoagulated.

Ukuhamba komsebenzi

Ukugcinwa kunye nobomi beshelufu

Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.

Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.

Isisombululo se-Foregene Protease Plus sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);iya kusebenza ngakumbi kwaye izinzile xa igcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuba igcinwe kwi-4 ° C, khumbula ukuba ungayigcini -20 ° C.

Ukudala ixabiso elithe chatha kubathengi yintanda-bulumko yenkampani yethu;Ukukhula komthengi kukuleqa iPhepha leXabiso leNtsha yeNtsholongwane yeNtsholongwane eNtsha engaBandeli ye-Viral DNA / i-Rna Nucleic Acid Extraction Kit Kit Reagent Kits yeXesha lokwenyani iPCR yegazi, Samkela ngeentliziyo ezipheleleyo abathengi kwihlabathi liphela beze kwindawo yethu yokuvelisa kwaye babe nentsebenziswano yokuphumelela kunye nathi!
IPhepha leXabiso leI-DNA yeGazi yaseTshayina kunye neKhiti yokuKhupha, Sithobela umxhasi woku-1, umgangatho ophezulu we-1, ukuphuculwa okuqhubekayo, inzuzo efanayo kunye nemigaqo yokuphumelela.Xa intsebenziswano kunye nomthengi, sihambisa abathengi ngeyona nkonzo iphezulu iphezulu.Ukuseka ubudlelwane obuhle beshishini sisebenzisa umthengi waseZimbabwe ngaphakathi kwishishini, sizenzele igama kunye nodumo.Kwangelo xesha linye, wamkele ngeentliziyo ezipheleleyo amathemba amatsha namadala kwinkampani yethu ukuya kuthethathethana namashishini amancinci.