Yintoni i-PCR eNgqo

I-PCR ethe ngqo yindlela ye-DNA yokukhulisa ngokuthe ngqo ukusukaigazi, inyama yezilwanyana,umsila wempuku,iseli, indlebe yempuku,zebra intlanzi, amaqanda entlanzi,tyala amagqabi,iimbewuokanye enye isampuli ye-biological tissue ye-original ngaphandle kokwenza i-DNA yodwa kunye namanyathelo okucoca.Ubuchule be-PCR obuthe ngqo bunokunciphisa kakhulu ixesha lovavanyo, kunye neendleko kwi-genotyping kunye neeprojekthi zobuninzi obuphezulu.Ikwabonelela ngenketho engcono xa ujongene nemingeni yokukhulisa inani elincinci kakhulu lesampulu apho inyathelo lokucoca linokukhokelela kwilahleko yesampulu.

I-ForEGENE's PCR Mix inoxhathiso oluqinileyo loxinzelelo, ngenxa ye-FOREGENE amandla anelungelo elilodwa lomenzi we-Taq enzyme ( ipatent egunyazisiweyo: ZL20161034512.0 (China), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)I-enzyme yayo ye-Taq isebenzisa iteknoloji yokudibanisa i-DNA ukuhlanganisa kwakhona i-DNA ebophelelayo ummandla wesakhiwo se-archaeal nucleic acid ebopha iprotheni kunye ne-Taq DNA polymerase enganyangekiyo ubushushu ukwakha i-template ye-DNA ngokunyuka kobudlelwane.I-Taq ephuculweyo eshushu-isiqalo egcina imisebenzi eyahlukeneyo ye-polymerase ye-DNA yasekuqaleni.Le enzyme ye-Taq inokubophelela kwi-template ye-DNA kwaye iqalise i-DNA synthesis kwi-templates ephantsi yoxinaniso kunye neendawo ezinzima.

For umzekelo,itkulula njengokongeza eyakhoiseli,isityalo (igqabi eliselula, ingcambu okanye imbewu) okanye isampuli yesilwanyana kwibuffer, kwaye ukongeza eyodwamaster mix kunyeethileii-primers kwiityhubhu ze-PCR kwaye uyiqhube ngebhayisekile ye-thermal.Ukusebenza kweKit kukulungele uhlalutyo lwesampulu enkulu apho ukugcinwa kwexesha kubaluleke kakhulu.

Inkqubo yokusabela kwe-PCR equlethe i-UNG enzyme kunye ne-dUTP ikhethwe ngokweemfuno zovavanyo, ezinokuthi ziphephe ngokufanelekileyo ukungcola okubangelwa yimveliso yokukhulisa i-PCR.