Sigxininisa kwithiyori yophuhliso 'Yokusemagqabini okuphezulu, ukuSebenza, ukunyaniseka kunye nendlela yokusebenza ephantsi' ukukuzisa ngomboneleli omkhulu wokusetyenzwa koMboneleli othembekileyo waseTshayina CE Ephawulwe kwiLab Reagent Nucleic Acid Magnetic Bead.Nucleic Acid Extraction Kits Rna Isolation DNA Ukuhlanjululwa, Injongo yethu kukukunceda wenze ubudlelwane obuhlala ixesha elide kunye nabathengi bakho ngamandla emveliso yokuthengisa.
Sigxininisa kwithiyori yophuhliso 'Yokugqwesa okuphezulu, ukuSebenza, ukunyaniseka kunye nendlela yokusebenza ephantsi' ukukuzisa ngomboneleli omkhulu wokusetyenzwa kweChina Nucleic Acid Magnetic Bead, Nucleic Acid Extraction Kit, Umgangatho ogqwesileyo uvela ekubambeleleni kwethu kuzo zonke iinkcukacha, kwaye ukwaneliseka kwabathengi kuvela ekuzinikezeleni kwethu ngokunyanisekileyo.Ngokuthembela kubuchwephesha obuphambili kunye nodumo kwishishini lentsebenziswano elungileyo, sizama konke okusemandleni ethu ukuba sinikele ngeempahla ezisemgangathweni kunye neenkonzo kubathengi bethu, kwaye sonke sizimisele ukomeleza utshintshiselwano kunye nabathengi basekhaya nabangaphandle kunye nentsebenziswano enyanisekileyo, ukwakha ikamva elingcono.

Iinkcukacha

50 Preps, 200 Preps

I-Viral RNA I-Nucleic Acid Ukucoca I-Isolation Kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA yentsholongwane kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid, kunye ne-cell culture supernatant.Ikiti yongeza ngokukodwa i-Linear Acrylamide, enokuthi ibambe ngokulula ixabiso elincinci le-RNA kwiisampuli.IKholam ye-RNA Kuphela inokubopha i-RNA ngokufanelekileyo.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Ikhithi yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer viRW1 kunye ne-Buffer viRW2 inokuqinisekisa ukuba i-viral nucleic acid efunyenweyo ayinaprotheyini, i-nuclease okanye enye into engcolileyo, enokusetyenziswa ngokuthe ngqo kwimifuniselo yebhayoloji yemolekyuli esezantsi.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Isivuno esiphezulu se-nucleic acid: I-DNA / RNA-iKholamu kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-DNA kunye ne-RNA.
■ Umthamo omkhulu wokusetyenzwa kwesampulu: ukuya kuthi ga kwi-200μl iisampulu zisenokwenziwa ngexesha ngalinye.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-20.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukukhutshwa kunye nokucocwa kwe-viral nucleic acid kwiisampuli ezifana ne-plasma, i-serum, i-cell-free body fluid kunye ne-cell culture supernatant.

Ukuhamba komsebenzi

Umzobo

Ugcino kunye nobomi beShelf

■ Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
■ Isisombululo seLinear Acrylamide sinokugcinwa kwiqondo lobushushu legumbi kangangeentsuku ezisi-7;emva kokufumana ikiti, nceda uyikhuphe kwaye uyigcine kwi-20°C.
■ Emva kokongeza i-Linear Acrylamide kwi-Buffer DRL, inokugcinwa kwi-2-8 ° C ukuya kwi-48h.Nceda usebenzise isisombululo esenziwe sele sigxininise kwithiyori yophuhliso 'egqwesileyo ephezulu, iNtsebenzo, ukunyaniseka kunye nezantsi-ukuya-earth yokusebenza indlela' ukukuzisa ngomboneleli omkhulu wokusetyenzwa koMboneleli othembekileyo waseTshayina CE Ephawulwe ngeLab Reagent yeNucleic Acid Magnetic Bead Nucleic Acid Extraction Kits Rna Isolation DNA Ukuhlanjululwa ngoncedo lwakho lokunyuswa kobudlelwane bomxumi kunye nobudlelwane bakho bomthengi.
UMboneleli othembekileyoChina Nucleic Acid Magnetic Bead, I-Nucleic Acid Extraction Kit, Umgangatho ogqwesileyo uvela ekubambeleleni kwethu kuzo zonke iinkcukacha, kwaye ukwaneliseka kwabathengi kuvela ekuzinikezeleni kwethu ngokunyanisekileyo.Ngokuthembela kubuchwephesha obuphambili kunye nodumo kwishishini lentsebenziswano elungileyo, sizama konke okusemandleni ethu ukuba sinikele ngeempahla ezisemgangathweni kunye neenkonzo kubathengi bethu, kwaye sonke sizimisele ukomeleza utshintshiselwano kunye nabathengi basekhaya nabangaphandle kunye nentsebenziswano enyanisekileyo, ukwakha ikamva elingcono.