Inkampani igcina ijolise kumbono wokusebenza "ulawulo lwesayensi, umgangatho ophezulu kunye nomgangatho wokusebenza, umxhasi ophakamileyo ngeXabiso eliKhethekileyo leChina iMagnetic Bead Method Swab Isampulu yeNucleic Acid Extraction Kit Rna Isolation Reagent DNA Purification Kit, Nucleic Acid Extraction Kit Rna Isolation DNA Extraction, Kuba sihlala kunye ne-10 yeminyaka yokutsalwa.Sifumene inkxaso yababoneleli abasebenzayo ngokugqwesileyo kunye nexabiso.Kwaye besibakhuphele ngaphandle abaxhasi abanomgangatho ophantsi ophezulu.Ngoku iifektri ezininzi ze-OEM zisebenzisana nathi.
Iqumrhu ligcina ingcamango yokusebenza "ulawulo lwenzululwazi, umgangatho ophezulu kunye nokubaluleka kokusebenza, umthengi ophakamileyoChina DNA Isolation Kit, Nucleic Acid Extraction Kit, sizimisele ngokupheleleyo ukulawula lonke ikhonkco lonikezelo ukuze sihambise iimpahla ezisemgangathweni ngexabiso elikhuphisanayo ngexesha elifanelekileyo.Siqhubeka nobuchule obuphambili, sikhula ngokudala amaxabiso angaphezulu kubathengi bethu nakuluntu.

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Inkampani igcina ijolise kumbono wokusebenza "ulawulo lwesayensi, umgangatho ophezulu kunye nomgangatho wokusebenza, umxhasi ophakamileyo ngeXabiso eliKhethekileyo leChina iMagnetic Bead Method Swab Isampulu yeNucleic Acid Extraction Kit Rna Isolation Reagent DNA Purification Kit, Nucleic Acid Extraction Kit Rna Isolation DNA Extraction, Kuba sihlala kunye ne-10 yeminyaka yokutsalwa.Sifumene inkxaso yababoneleli abasebenzayo ngokugqwesileyo kunye nexabiso.Kwaye besibakhuphele ngaphandle abaxhasi abanomgangatho ophantsi ophezulu.Ngoku iifektri ezininzi ze-OEM zisebenzisana nathi.
Ixabiso eliKhethekileyo le-China DNA Isolation Kit, i-Nucleic Acid Extraction Kit, sizimisele ngokupheleleyo ukulawula lonke ikhonkco lokubonelela ukuze kuhanjiswe urhwebo olusemgangathweni ngexabiso elikhuphisanayo ngexesha elifanelekileyo.Siqhubeka nobuchule obuphambili, sikhula ngokudala amaxabiso angaphezulu kubathengi bethu nakuluntu.