Ukubambelela kwinkolelo "yokwenza izinto eziphezulu zoluhlu kunye nokwenza abahlobo kunye nabantu namhlanje abavela kwihlabathi lonke", sihlala sibeka umdla wabathengi kwindawo yokuqala ye-Supply ODM China CE FDA IyahambelanaI-Nucleic Acid ExtractionIimvavanyo zeReagent Kit 96, Xa uzingela kanye kunye nomgangatho ophezulu ngexabiso elikhulu kunye nokuhanjiswa ngexesha elifanelekileyo.Nceda uqhagamshelane nathi.
Ukubambelela kwinkolelo "yokwenza izinto eziphezulu kuluhlu kunye nokwenza abahlobo nabantu namhlanje abavela kwihlabathi liphela", sihlala sibeka umdla wabathengi kwindawo yokuqala.China Nucleic Acid Extraction Reagent, I-Nucleic Acid Extraction, Ngoku sinemisebenzi engaphezulu kwe-100 kwiplanti, kwaye sikwanayo neqela le-15 labafana abasebenza kwinkonzo yabathengi bethu ngaphambi nasemva kokuthengisa.Umgangatho olungileyo yeyona nto iphambili kwinkampani ukuba yahluke kwabanye abantu esikhuphisana nabo.Ukubona kukukholelwa, ufuna ulwazi oluninzi?Ukuvavanywa nje kwempahla yayo!

Inkcazo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho).

Ukubambelela kwinkolelo "Ukudala izinto eziphezulu kunye nokudala abahlobo kunye nabantu namhlanje abavela kwihlabathi lonke", ngokuqhelekileyo sibeka umdla wabathengi kwindawo yokuqala ye-Supply ODM China CE FDA Compatible Nucleic Acid Extraction Reagent Kit 96 Tests, Xa uzingela kanye kunye nomgangatho ophezulu kwithegi yexabiso elikhulu kunye nokuhanjiswa kwexesha.Nceda uqhagamshelane nathi.
Ukubonelela nge-ODMChina Nucleic Acid Extraction Reagent, I-Nucleic Acid Extraction, Ngoku sinemisebenzi engaphezulu kwe-100 kwisityalo, kwaye sikwanayo neqela elisebenzayo le-15 abafana ukuba bakhonze abathengi bethu ngaphambi nangemva kokuthengisa.Umgangatho olungileyo yeyona nto iphambili kwinkampani ukuba yahluke kwabanye abantu esikhuphisana nabo.Ukubona kukukholelwa, ufuna ulwazi oluninzi?Ukuvavanywa nje kwempahla yayo!