Sizibophelele ekuboneleleni ngokulula, ukonga ixesha kunye nokugcina imali enye yokuthenga inkonzo yokuthenga kuMgangatho oPhezulu weTechstar Nucleic Acid Extraction System ethengiswayo uMvelisi weNucleic Acid Series ovela eTshayina, sijonge phambili ekufumaneni imibuzo yakho ngokukhawuleza kwaye sinethemba lokuba nethuba lokufumana umsebenzi kunye nawe kwixa elizayo.Wamkelekile ukuba ufumane ukubonakala kumbutho wethu.
Sizibophelele ekunikezeleni ngeenkonzo ezilula, ezonga ixesha kunye nokugcina imali kwindawo enye yokuthenga abathengi.China Nucleic Acid Extraction System, Nucleic Acid Extraction Instrument, Ngeyona nkxaso ibalaseleyo yetekhnoloji, siye sayilungisa iwebhusayithi yethu ukuze sifumane amava angcono omsebenzisi kwaye sikugcine engqondweni ukukhululeka kwakho ekuthengeni.siqinisekisa ukuba okona kulungileyo kufikelela kuwe emnyango wakho, ngexeshana elifutshane kwaye ngoncedo lwamaqabane ethu asebenzayo okusebenza oko kukuthi i-DHL kunye ne-UPS.Sithembisa umgangatho, siphila ngokwesaci sokuthembisa kuphela oko sinokukuphumeza.

Inkcazo

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Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho).

Sizibophelele ekuboneleleni ngokulula, ukonga ixesha kunye nokugcina imali enye yokuthenga inkonzo yokuthenga kuMgangatho oPhezulu weTechstar Nucleic Acid Extraction System ethengiswayo uMvelisi weNucleic Acid Series ovela eTshayina, sijonge phambili ekufumaneni imibuzo yakho ngokukhawuleza kwaye sinethemba lokuba nethuba lokufumana umsebenzi kunye nawe kwixa elizayo.Wamkelekile ukuba ufumane ukubonakala kumbutho wethu.
Umgangatho ophezuluChina Nucleic Acid Extraction System, Nucleic Acid Extraction Instrument, Ngeyona nkxaso ibalaseleyo yetekhnoloji, siye sayilungisa iwebhusayithi yethu ukuze sifumane amava angcono omsebenzisi kwaye sikugcine engqondweni ukukhululeka kwakho ekuthengeni.siqinisekisa ukuba okona kulungileyo kufikelela kuwe emnyango wakho, ngexeshana elifutshane kwaye ngoncedo lwamaqabane ethu asebenzayo okusebenza oko kukuthi i-DHL kunye ne-UPS.Sithembisa umgangatho, siphila ngokwesaci sokuthembisa kuphela oko sinokukuphumeza.