Sihlala sicinga kwaye siziqhelanisa ngokuhambelana nokutshintsha kweemeko, kwaye sikhule.Sinenjongo yokuphumelela ingqondo nomzimba otyebileyo kunye nokuphila kubathengisi beWholesale yeProfessional Magnetic Viral Rna Nucleic Acid Isolation Prepacked Kits, Ifemu yethu isebenza ngomgaqo-nkqubo "wokusekwe kwintembeko, intsebenziswano eyenziweyo, abantu abaqhelanisiweyo, baphumelele intsebenziswano".Siyathemba ukuba ngokulula sinokuba nentsebenziswano emnandi kunye nosomashishini abasuka kuzo zonke iindawo ezisingqongileyo.
Sihlala sicinga kwaye siziqhelanisa ngokuhambelana nokutshintsha kweemeko, kwaye sikhule.Sinenjongo ekufezekiseni ingqondo nomzimba otyebileyo kunye nokuphilaI-China Universal Rna DNA Reagents kunye ne-Automatic Nucleic Acid Purification, Sinqwenela ukuhlangabezana neemfuno zabathengi bethu kwihlabathi jikelele.Uluhlu lwethu lwentengiso kunye neenkonzo zikhula ngokuqhubekayo ukuhlangabezana neemfuno zabathengi.Samkela abathengi abatsha nabadala abavela kuzo zonke iinkalo zobomi ukuba baqhagamshelane nathi kubudlelwane beshishini elizayo kunye nokuzuza impumelelo efanayo!
Izikhokelo zoMyalelo:

 Sihlala sicinga kwaye siziqhelanisa ngokuhambelana nokutshintsha kweemeko, kwaye sikhule.Sinenjongo yokuphumelela ingqondo nomzimba otyebileyo kunye nokuphila kubathengisi beWholesale yeProfessional Magnetic Viral Rna Nucleic Acid Isolation Prepacked Kits, Ifemu yethu isebenza ngomgaqo-nkqubo "wokusekwe kwintembeko, intsebenziswano eyenziweyo, abantu abaqhelanisiweyo, baphumelele intsebenziswano".Siyathemba ukuba ngokulula sinokuba nentsebenziswano emnandi kunye nosomashishini abasuka kuzo zonke iindawo ezisingqongileyo.
Abathengisi beWholesale yeI-China Universal Rna DNA Reagents kunye ne-Automatic Nucleic Acid Purification, Sinqwenela ukuhlangabezana neemfuno zabathengi bethu kwihlabathi jikelele.Uluhlu lwethu lwentengiso kunye neenkonzo zikhula ngokuqhubekayo ukuhlangabezana neemfuno zabathengi.Samkela abathengi abatsha nabadala abavela kuzo zonke iinkalo zobomi ukuba baqhagamshelane nathi kubudlelwane beshishini elizayo kunye nokuzuza impumelelo efanayo!

Isikhokelo sokuHlalutya ingxaki

Olu lucazululo lulandelayo lweengxaki onokudibana nazoIzityalo zizonkeRNA extraction will help you with your experiments. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali : Tech@foregene.com.

Uluhlu olujikelezayo luvalekile

Ukuvinjwa kwekholomu ye-spin kuya kubangela ukuba isivuno se-RNA sincitshiswe okanye singakwazi ukuhlanjululwa ukufumana i-RNA, kwaye umgangatho we-RNA efunyenweyo uya kuba phantsi.

Uhlalutyo lwesizathu esiqhelekileyo:

1. Isampuli ayiphulwanga ngokupheleleyo.

Ukuqhekeka kwesampulu engaphelelanga kunokuthintela i-DNA-Cleaning Column, enokuthi ichaphazele isivuno se-RNA kunye nomgangatho.Sincoma ukuba xa usenza ulwahlulo lwesampulu, ngokukhawuleza ugaye kwinani elaneleyo le-nitrogen engamanzi ukuqhekeza izicubu ezinje ngeendonga zeseli kunye neembrane zeeseli zeesampulu kangangoko kunokwenzeka.Kwiisampuli zezityalo ze-polyphenol polysaccharides, sincoma ukuba usebenzise i-Plant Total RNA Isolation Kit Plus.

2.Aspirate DNA-Cleaning Column yedwa supernatant, aspirate kunokwenzeka cell debris pellet.

I-aspirated cell debris pellet inokuvala iKholamu ye-RNA kuphela ngexesha leenkqubo ze-adsorption ye-RNA (jonga inyathelo lesi-5 lenkqubo, inyathelo lesi-6 lenkqubo ye-polysaccharide polyphenol).Sicebisa ukuba unonophelo kufuneka luthathwe xa unqwenela lo mandla angaphezu kwamandla ukuze uthintele i-debris cell debris.

3. Isixa sokuqala sesampulu sikhulu kakhulu.

Ukusetyenziswa kwesampulu ngokugqithisileyo kuya kubangela ukwahlulwa kwesampulu engaphelelanga okanye uhlaziyo olungaphelelanga lweeseli nge-Buffer PRL1 okanye i-Buffer PSL1, ekhokelela kuluhlu oluvaliweyo lokucoca lwemisebenzi yokucoca.I-Plant Total RNA Isolation Kit inomlinganiselo wokuqala we-50 mg ngokucocwa okukodwa kwesampuli esebenzayo.Kwiisampuli zezityalo ze-polyphenol polysaccharides, sincoma ukuba uzame i-Plant Total RNA Isolation Kit Plus.

4. Ubushushu be-centrifuge buphantsi kakhulu.

Ukwahlulwa okupheleleyo kwe-RNA kunye nokucocwa ngaphandle kokuphazamiseka kwenitrogen ye-liquid yesampula yezicubu, onke amanyathelo enziwa kwiqondo lobushushu legumbi (20-25 °C).Ezinye ii-centrifuge zobushushu obuphantsi zinamaqondo obushushu angaphantsi kwe-20 °C, nto leyo enokubangela imiqobo kwiKholam yokucoca ye-DNA kunye/okanye iKholamu ye-RNA kuphela.Ukuba oku kuyenzeka, seta ubushushu be-centrifuge ukuya kuma-20-25 °C kwaye ufudumeze umxube we-lysis kunye / okanye ukongezwa kwe-ethanol yokwahlula ngaphezulu kwe-37 °C.

Akukho RNA ekhutshiweyo okanye isivuno se-RNA siphantsi

Ngokuqhelekileyo kukho izinto ezininzi ezichaphazela ukusebenza kakuhle kokubuyisela, njenge: umxholo we-RNA yesampuli, indlela yokusebenza, umthamo we-lution, njl.

Uhlalutyo lwezizathu eziqhelekileyo zingezantsi:

I-1.I-ice bath okanye ukushisa okuphantsi (4 ° C) i-centrifugation yenziwa ngexesha lokusebenza.

Isiphakamiso: Sebenza kwiqondo lokushisa (15-25 ° C) kuyo yonke inkqubo, musa ukwenza i-ice bath kunye ne-centrifugation yokushisa ephantsi.

       2.I-RNA iye yathotywa ngenxa yokugcinwa okungafanelekanga kwesampuli okanye ukugcinwa kwexesha elide lesampuli.

Isindululo: Iisampulu ezisandula ukuqokelelwa kufuneka zikhe zikhenkcezwe ngokukhawuleza kwinitrogen engamanzi, emva koko zigcinwe ku -80°C ixesha elide, zithintele ukuphinda kukhenkceze kunye nokunyibilika kweisampulu;okanye ngokukhawuleza ucwilise iisampuli kwi-RNA stabilizer RNAlater isisombululo (iisampulu zezilwanyana).

       3.Ukwahlulwa kwesampulu eyaneleyo kunye ne-lysis ikhokelela ekuvinjweni kwekholamu yokucoca.

Isiphakamiso: Xa ugaya i-tissue, nceda uqinisekise ukuba i-tissue iphantsi ngokwaneleyo, kwaye ngokukhawuleza uyidlulisele kwi-Buffer ye-PSL1 elungiselelwe ngaphambili (qinisekisa ukuba umlinganiselo ochanekileyo we-β-ME wongezwe, jonga inyathelo 1 lenkqubo).

4.I-eluent yongezwa ngokungalunganga.

Ingcebiso: Qinisekisa ukuba iRNase-Free ddH₂I-O ithontsizwa phakathi kwe-membrane yekholomu yokucoca.

5.Umthamo ochanekileyo we-ethanol epheleleyo ayizange yongezwe kwi-Buffer PSL2 okanye i-Buffer PRW2.

Ingcebiso: Nceda ulandele imiyalelo, yongeza umthamo ochanekileyo we-ethanol epheleleyo kwi-Buffer PSL2 kunye ne-Buffer PRW2 kwaye udibanise kakuhle phambi kokuba ikhithi isetyenziswe.

6.Ubungakanani besampulu yethishu ayifanelekanga.

Ingcebiso: Sebenzisa i-50 mg yethishu nge-500 μl ye-Buffer PSL1.Ukusebenzisa izicubu ezininzi kakhulu kuya kunciphisa inani le-RNA ekhutshiweyo kwaye ukucoceka kwe-RNA okubangelwayo kuya kuncitshiswa.Sincoma kakhulu ukuba idosi yesampulu yokuqala ayifanele idlule i-50 mg ngomsebenzi wokutsalwa kwe-RNA.

7. Umthamo wesandi esingafanelekanga okanye i-elution engaphelelanga.

Isiphakamiso: Umthamo ocacileyo wekholamu yokucoca ngu-50-200 μl;ukuba isiphumo se-elution asanelisi, kuyacetyiswa ukuba kwandiswe ixesha kwiqondo lobushushu legumbi emva kokongeza i-RNase-Free ddH efudunyeziweyo.₂O, njenge-5-10min.

8.Ikholamu yokucoca inentsalela ye-ethanol emva kokuhlamba nge-Buffer PRW2.

   Isiphakamiso: Ukuba ityhubhu engenanto i-centrifuged ye-1 min kwaye kusekho i-ethanol eseleyo emva kokuhlamba kwi-Buffer PRW2, unokwandisa ixesha le-tube engenanto ye-centrifugation ukuya kwi-2 min, okanye ubeke ikholamu yokucoca kwindawo yokushisa ye-5 min ukususa ngokupheleleyo i-ethanol eseleyo.

9.Ikhithi isetyenziswe ngokungachanekanga.

   Isiphakamiso: Kwiisampuli zezityalo ze-polyphenolic polysaccharides, ukusebenzisa iikiti eziqhelekileyo ezifana ne-Plant Total RNA Isolation Kit ayinakukwazi ukufumana iisampulu ezifanelekileyo ze-RNA.Sincoma ukuba usebenzise iPlant Total RNA IsolationKit Plus, eyilelwe ngokukodwa iisampuli zezityalo ze-polyphenolic polysaccharide.Ikhithi ephuhliswe ngokukodwa ukukhupha i-RNA kwi-polyphenol kunye neesampuli zezityalo ze-polysaccharide.

Ixabiso le-OD260/OD280 liphantsi

I-RNA elution kunye ne-ddH₂I-O kwaye isetyenziselwa ukufundwa kwe-spectrophotometer iphumela kwixabiso eliphantsi le-OD260/OD280.Sincoma ukusebenzisa i-10 mM Tris-HCl, pH 7.5 (kunokuba RNase-Free ddH₂O to elute RNA) ukufumana amaxabiso achanekileyo eOD260/OD280, bona “RNA Concentration and Purification Assays” kwiphepha le-19.

I-RNA ehlanjululweyo ithotywa

Umgangatho we-RNA ecocekileyo inxulumene nezinto ezifana nokugcinwa kwesampulu, ukungcoliseka kwe-RNase, kunye nokukhwabanisa.

Uhlalutyo lwezizathu eziqhelekileyo:

I-1.Iisampulu ze-tissue azizange zigcinwe ngexesha emva kokuqokelela.

    Isincomo: Ukuba iisampulu zezicubu azisetyenziswanga ngexesha emva kokuqokelela, nceda uzigcine kwi-nitrogen engamanzi kwiqondo lokushisa eliphantsi ngokukhawuleza okanye uzidlulisele kwi--80 ° C yokugcina ixesha elide emva kokuqhwala ngokukhawuleza kwi-nitrogen engamanzi, okanye ngokukhawuleza ucwilise iisampuli kwi-RNA stabilizer RNAlater isisombululo (iisampulu zezilwanyana).Ukukhutshwa kwe-RNA, zama ukusebenzisa iisampulu zeethishu ezisanda kuqokelelwa.

2.Ukukhenkceza okuphindaphindiweyo kunye nokunyibilika kweesampuli zethishu.

   Icebiso: Xa ugcina iisampulu zethishu, kungcono ukuzisika zibe ziingceba ezincinci ukuze zigcinwe, kwaye ukhuphe inxalenye yazo xa uzisebenzisa ukuthintela ukonakaliswa kweRNA okubangelwa kukukhenkcezwa okuphindaphindiweyo nokunyibilika kweisampulu.

3.I-RNase yaziswa kwigumbi lokusebenza okanye inganxitywanga iiglavu ezilahlwayo, iimaski, njl.

   Ingcebiso: Imifuniselo yokutsalwa kwe-RNA yenziwa ngcono kwimisebenzi eyahlukeneyo ye-RNA, kwaye itafile yelabhoratri kufuneka icocwe phambi kovavanyo, kwaye iiglavu ezilahlwayo kunye nemaski kufuneka zinxitywe ngexesha lovavanyo ukunqanda ukuthotywa kwe-RNA okubangelwa kukwaziswa kwe-RNase ngowona mgangatho mkhulu.

I-4.I-reagent ingcolisekile yi-RNase ngexesha lokusetyenziswa.

   Ingcebiso: Faka endaweni yoluhlu olutsha lwezixhobo ze-RNA zokutsalwa kwezityalo zizonke zemifuniselo enxulumeneyo.

I-5.Iibhubhu ze-centrifuge kunye neengcebiso ze-pipette ezisetyenziselwa ukuguqulwa kwe-RNA zingcolile nge-RNase.

Ingcebiso: Qinisekisa ukuba iityhubhu ze-centrifuge, iingcebiso ze-pipette, ii-pipettes, njl njl. ezisetyenziswa kwi-RNA extraction zonke zi-RNase-Free.

Izikhokelo zoMyalelo:

ISityalo sisonke seRNA yeSikhokelo seKit yoMyalelo