Mhlawumbi sinezona zixhobo zikumgangatho ophezulu zemveliso, iinjineli kunye nabasebenzi abanamava kunye nabaqeqeshiweyo, baye baqaphela umgangatho olungileyo wokulawula iinkqubo kunye nenkxaso yabasebenzi abanezakhono zokufumana umvuzo wangaphambi / emva kokuthengisa kwi-CE Certificate China 16 Isolation Reagent Viral DNA/Rna Purification Kit.
Mhlawumbi sinezona zixhobo zikumgangatho ophezulu zemveliso, iinjineli ezinamava naqeqeshiweyo kunye nabasebenzi, iinkqubo zolawulo ezikumgangatho olungileyo kunye nenkxaso yabasebenzi abanobungane abanezakhono phambi / emva kokuthengisa.China Nucleic Acid Ukutsalwa kweReagent Kit, IKit Nucleic Acid Extraction Reagent Kit, Sibambelele kumgangatho obalaseleyo, ixabiso elikhuphisanayo kunye nokufika ngexesha kunye nenkonzo engcono, kwaye sithemba ngokunyanisekileyo ukuseka ubudlelwane obuhle bexesha elide kunye nentsebenziswano kunye namaqabane ethu amatsha kunye namadala asuka kwihlabathi jikelele.Wamkelekile ngokunyanisekileyo ukuba uzibandakanye nathi.

Iinkcukacha

50 Preps, 200 Preps

Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 ingagcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) mhlawumbi sinezona zixhobo zemveliso zaseburhulumenteni, iinjineli ezinamava kunye nabasebenzi abaqeqeshiweyo, baqaphela iinkqubo zokulawula umgangatho olungileyo kunye nenkxaso yabasebenzi abanobungane abanezakhono ngaphambi / emva kokuthengiswa kweCertificate ye-CE Certificate kunye ne-ReanaR ngokuqhubekayo. ukuseka ubudlelwane nababoneleli abatsha ukubonelela abathengi bethu abaxabisekileyo ngeendlela eziqhubekayo nezikrelekrele.
Isatifikethi seCEChina Nucleic Acid Ukutsalwa kweReagent Kit, IKit Nucleic Acid Extraction Reagent Kit, Sibambelele kumgangatho obalaseleyo, ixabiso elikhuphisanayo kunye nokufika ngexesha kunye nenkonzo engcono, kwaye sithemba ngokunyanisekileyo ukuseka ubudlelwane obuhle bexesha elide kunye nentsebenziswano kunye namaqabane ethu amatsha kunye namadala asuka kwihlabathi jikelele.Wamkelekile ngokunyanisekileyo ukuba uzibandakanye nathi.