Siye saba ngumenzi onamava.Ukuphumelela uninzi lwezatifikethi zakho ezibalulekileyo zentengiso ye-Super Purchasing ye-China yeXesha lokwenyani i-PCR, Ukuba unomdla phantse kuzo naziphi na iimpahla, khumbula ukuziva ukhululekile ngokupheleleyo ukunxibelelana nathi malunga neenyani ezithe vetshe okanye qiniseka ukuba usithumelele i-imeyile ekunene, siya kukuphendula nje kwiiyure ezingama-24 kunye neyona kowuteshini intle siza kuyinikwa.
Siye saba ngumenzi onamava.Ukuphumelela uninzi lwezatifiketi zakho ezibalulekileyo kwimarike yayoChina PCR, Ixesha lokwenyani Pcr, Ngomoya woshishino "wobuchule obuphezulu, obulula, obusebenzisekayo kunye nokusungula izinto ezintsha", kwaye ngokuhambelana nesikhokelo sokukhonza "somgangatho olungileyo kodwa ixabiso elingcono," kunye "netyala lehlabathi", sizama ukusebenzisana neenkampani zamacandelo emoto kwihlabathi liphela ukwenza ubambiswano oluphumeleleyo.

Iinkcazelo

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo enokuthi ikhulule ngokukhawuleza i-RNA kwiisampulu zeseli ezikhuliswe kwiimpendulo ze-RT-qPCR, ngaloo ndlela iphelisa inkqubo yokucoca i-RNA ethatha ixesha kunye nenzima.Ithemplethi ye-RNA inokufumaneka kwimizuzu nje esi-7.Umxube we-RT oyi-5 × ngokuthe ngqo kunye ne-2 × Direct qPCR Mix-SYBR ii-reagents ezibonelelwe yikhithi zinokufumana ngokukhawuleza nangempumelelo iziphumo zePCR zexesha lokwenyani.

I-5 × Direct RT Mix kunye ne-2 × Direct qPCR Mix-SYBR inokunyamezela inhibitor eqinile, kunye ne-lysate yeesampuli zingasetyenziswa njenge template ye-RT-qPCR ngokuthe ngqo.Le khithi iqulethe i-RNA ephezulu ehambelana ne-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl₂, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.

Iinkcukacha

200×20μl Rxns, 1000×20μl Rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ilula kwaye isebenzayo : ngeteknoloji ye-Cell Direct RT, iisampuli ze-RNA zinokufumaneka kwimizuzu nje eyi-7.

∎ Imfuno yesampulu incinci, ingaphantsi njengeeseli ezili-10 zinokuvavanywa.

■ Ukugqithisa okuphezulu: inokubona ngokukhawuleza i-RNA kwiiseli ezikhuliswe kwii-384, 96, 24, 12, 6-well plates.

■ I-DNA Eraser inokususa ngokukhawuleza iigenomes ezikhutshiweyo, inciphise kakhulu impembelelo kwiziphumo zovavanyo ezilandelayo.

■ Inkqubo elungiselelwe i-RT kunye ne-qPCR yenza amanyathelo amabini e-RT-PCR akhutshelwe umva asebenze ngakumbi kwaye i-PCR icace ngakumbi, kwaye imelane ngakumbi ne-RT-qPCR reaction inhibitors.

Isicelo seKit

Ububanzi besicelo: iiseli ezikhuliswe.

- I-RNA ekhutshwe ngesampuli ye-lysis: isebenza kuphela kwi-template ye-RT-qPCR yale khithi.

- Ikiti ingasetyenziselwa ezi njongo zilandelayo: uhlalutyo lwe-gene expression, ukuqinisekiswa kwe-siRNA-mediated gene silence effect, ukuhlolwa kweziyobisi, njl.

Umzobo

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-4℃;Icandelo II kufuneka ligcinwe kwi -20 ℃.

I-Foregene Protease Plus II kufuneka igcinwe ku-4℃, ungakhenkcezisi ku -20℃.

I-Reagent 2 × Direct qPCR Mix-SYBR kufuneka igcinwe kwi -20 ℃ ebumnyameni;ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (sebenzisa kwiintsuku ezili-10) sinamava omvelisi.Ukuphumelela uninzi lwezatifikethi zakho ezibalulekileyo kwimakethi yayo yokuThenga okuPhezulu kweTshayina Q160/Q320 yeXesha lokwenyani PCR, Ukuba unomdla phantse kuyo nayiphi na impahla, khumbula ukuziva ukhululekile ngokupheleleyo ukuba uqhagamshelane nathi ngeenyani ezithe vetshe okanye qiniseka ukuba usithumelele i-imeyile ekunene, siya kukuphendula kwiiyure nje ezingama-24 kwaye neyona khowuwuti iya kubonelelwa.
Ukuthenga okuphezulu kweChina PCR, I-PCR yexesha lokwenyani, Ngomoya osebenzayo wobuchule obuphezulu, ubulula, obusebenzayo kunye nokusungula izinto ezintsha, kwaye ngokuhambelana nesikhokelo sokukhonza "somgangatho olungileyo kodwa ixabiso elingcono," kunye "netyala lehlabathi", sizama ukusebenzisana neenkampani zamacandelo emoto kwihlabathi liphela ukwenza ubambiswano lokuphumelela.