Sinokwanelisa ngokulula abathengi bethu abahlonitshwayo ngomgangatho wethu ophezulu, ixabiso lokuthengisa eligqwesileyo kunye nenkonzo entle ngenxa yokuba siye saziingcali ngakumbi kwaye sisebenza nzima kwaye siyenza ngendlela enexabiso eliphantsi yeFactory ethengisa i-China Viral Magnetic Beads Virus Nucleic Acid DNA Rna Extraction Kit, Samkela abathengi abatsha nabaphelelwe lixesha ukuba benze unxibelelwano olude kunye nonxibelelwano olude oluphuma kuzo zonke iindlela zonxibelelwano kunye nonxibelelwano olude olunempumelelo!
Sinokwanelisa ngokulula abathengi bethu abahlonitshwayo ngomgangatho wethu ophezulu obalaseleyo, ixabiso elithengisiweyo elincomekayo kunye nenkonzo entle ngenxa yokuba siye saziingcali ngakumbi kwaye sisebenza nzima kwaye siyenza ngendlela eneendleko eziphantsi.China RNA Extraction Kit, Dna Rna Extraction Kit, Ngomoya "womgangatho ophezulu ubomi benkampani yethu;igama elihle yingcambu yethu”, sithemba ngokunyanisekileyo ukusebenzisana nabathengi abavela ekhaya nakumazwe aphesheya kwaye sinethemba lokwakha ubudlelwane obuhle nawe.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sinokwanelisa ngokulula abathengi bethu abahlonitshwayo ngomgangatho wethu ophezulu, ixabiso lokuthengisa eligqwesileyo kunye nenkonzo entle ngenxa yokuba siye saziingcali ngakumbi kwaye sisebenza nzima kwaye siyenza ngendlela enexabiso eliphantsi yeFactory ethengisa i-China Viral Magnetic Beads Virus Nucleic Acid DNA Rna Extraction Kit, Samkela abathengi abatsha nabaphelelwe lixesha ukuba benze unxibelelwano olude kunye nonxibelelwano olude oluphuma kuzo zonke iindlela zonxibelelwano kunye nonxibelelwano olude olunempumelelo!
Umzi-mveliso othengisa i-China Rna Extraction Kit, i-DNA Rna Extraction Kit, Ngomoya “womgangatho ophezulu bubomi benkampani yethu;igama elihle yingcambu yethu”, sithemba ngokunyanisekileyo ukusebenzisana nabathengi abavela ekhaya nakumazwe aphesheya kwaye sinethemba lokwakha ubudlelwane obuhle nawe.