Isidima soMsebenzisi esiLungileyo seChina iNucleic Acid yeKhithi yokutsalwa kwegazi leDNA yoVavanyo lweNucleic Acid yokuCoca iReagent
Siyonwatyiswa kudumo oluhle ngendlela emangalisayo phakathi kwabathengi bethu ngemveliso yethu ekumgangatho ophezulu, isantya esindlongo-ndlongo kunye neyona nkxaso isebenzayo yoSidima soMsebenzisi oLungileyo waseTshayina iNucleic Acid yeKhithi yokuTyangwa kweGazi le-DNA yeNucleic Acid yokuCoca iReagent, amathemba kuqala!Nokuba yintoni na oyifunayo, kufuneka senze konke okusemandleni ethu ukukunceda.Siyabamkela ngokufudumeleyo abathengi abavela kwihlabathi liphela ukuba basebenzisane nathi ukukhulisana.
Sifumana ukonwabela ukuthandwa okuhle okumangalisayo phakathi kwabathengi bethu kurhwebo lwethu olukumgangatho ophezulu, izinga elinobundlongondlongo kunye neyona nkxaso isebenzayoChina Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Sineenjineli eziphezulu kula mashishini kunye neqela elisebenzayo kuphando.Ngaphezu koko, ngoku sinemilomo yethu yoovimba kunye neentengiso eTshayina ngexabiso eliphantsi.Ke ngoko, sinokudibana nemibuzo eyahlukeneyo evela kubathengi abahlukeneyo.Khumbula ukufumana iwebhusayithi yethu ukujonga ulwazi oluninzi kwizinto zethu.
Iinkcazelo Kits
50 Preps, 200 Preps
Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.
Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.
Amacandelo ekhithi:
Buffer RL1 , Buffer RL2 |
Isithinteli i-RW1, i-Buffer RW2 |
RNase-Free ddH2O, DNA-Ukucoca Column |
Ikholamu ye-RNA Kuphela |
Iimpawu&izinto eziluncedo
■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1
Isicelo seKit
Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.
Iiparamitha zemveliso
■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×106
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl
Ukuhamba komsebenzi
Umzobo
Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar
Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo
Ukugcinwa kunye nobomi beshelufu
Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).
Siyonwatyiswa kudumo oluhle ngendlela emangalisayo phakathi kwabathengi bethu ngemveliso yethu ekumgangatho ophezulu, isantya esindlongo-ndlongo kunye neyona nkxaso isebenzayo yoSidima soMsebenzisi oLungileyo waseTshayina iNucleic Acid yeKhithi yokuTyangwa kweGazi le-DNA yeNucleic Acid yokuCoca iReagent, amathemba kuqala!Nokuba yintoni na oyifunayo, kufuneka senze konke okusemandleni ethu ukukunceda.Siyabamkela ngokufudumeleyo abathengi abavela kwihlabathi liphela ukuba basebenzisane nathi ukukhulisana.
Udumo lomsebenzisi olulungileyo lweChina Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Sineenjineli eziphezulu kula mashishini kunye neqela elisebenzayo kuphando.Ngaphezu koko, ngoku sinemilomo yethu yoovimba kunye neentengiso eTshayina ngexabiso eliphantsi.Ke ngoko, sinokudibana nemibuzo eyahlukeneyo evela kubathengi abahlukeneyo.Khumbula ukufumana iwebhusayithi yethu ukujonga ulwazi oluninzi kwizinto zethu.