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Iseli ngqo ye-RT qPCR Kit
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer enokuthi ikhuphe i-RNA ngokukhawuleza kwiisampulu zeseli ezenzelwe i-RT-qPCR reaction, ngaloo ndlela ishenxisa inkqubo yokuhlanjululwa kwexesha kunye ne-RNA. Itemplate yeRNA inokufumaneka kwimizuzu nje esi-7. Umxube we-5 × ngqo we-RT kunye ne-2 × ngqo qPCR Mix-SYBR reagents ebonelelwe yikiti inokufumana ngokukhawuleza nangokufanelekileyo iziphumo ze-PCR zexesha lokwenyani.
I-5 × i-Direct RT Mix kunye ne-2 × i-Direct QPCR Mix-SYBR ine-inhibitor eyomeleleyo yokunyamezelana, kwaye i-lysate yeesampulu inokusetyenziswa njenge template ye-RT-qPCR ngqo. Le khithi iqulethe i-RNA ekhethekileyo yokuhlangana okuphezulu kwe-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.
