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Tyalela igqabi ngokuthe ngqo kwikhithi yePCR Plus
Le mveliso isebenzisa inkqubo ye-lysis buffer eyahlukileyo yokwenza amagqabi ezityalo. I-lysate inokusetyenziswa njengetemplate ngaphandle kokuhlanjululwa. Emva kokongeza ii-primers, i-PCR Mix yale kit ingasetyenziselwa ukukhulisa.
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Tyala iMbewu ngqo kwiPCR Kit I / II
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer yokufaka imbewu yezityalo. I-lysate inokusetyenziswa njengetemplate ngaphandle kokuhlanjululwa. Emva kokongeza ii-primers, i-PCR Mix yale kit ingasetyenziselwa ukukhulisa.
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Tyala igqabi ngqo PCR kunye nekhithi-UNG
Ngokusekwe kwikhithi ye-Plant Leaf ngqo ye-PCR Plus, i-DUTP iyasetyenziswa endaweni ye-dTTP, kunye ne-enzyme ye-UNG enokuthomalalisa itemplate ene-DUTP. Ngale ndlela, ukucaciswa kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yongcoliseko lwemveliso yePCR enokuthi yenzeke ngexesha lovavanyo olukhulu lwemfuza kuthintelwe.
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Tyalela igqabi ngqo kwi-PCR kit-UNG
Ngokusekwe kwikhithi ye-Plant Leaf ngqo ye-PCR, i-DUTP iyasetyenziswa endaweni ye-dTTP, kunye ne-enzyme ye-UNG enokuthomalalisa itemplate ene-DUTP. Ngale ndlela, ukucaciswa kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yongcoliseko lwemveliso yePCR enokuthi yenzeke ngexesha lovavanyo olukhulu lwemfuza kuthintelwe.
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Tyala igqabi ngqo kwikhithi yePCR
Le mveliso isebenzisa inkqubo ye-lysis buffer eyahlukileyo yokwenza amagqabi ezityalo. I-lysate inokusetyenziswa njengetemplate ngaphandle kokuhlanjululwa. Iipremers zinokongezwa kwaye Umxube wePCR wale khithi unokusetyenziselwa ukukhulisa.
