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Inkqubo ePhakamileyo yaseChina yeNyukliya yokuCoca iAcid, Umatshini wokuCoca ngokuCoca iAcid oZenzekelayo, i-32 kunye ne-96 Type CE
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer enokuthi ikhuphe i-RNA ngokukhawuleza kwiisampulu zeseli ezenzelwe i-RT-qPCR reaction, ngaloo ndlela ishenxisa inkqubo yokuhlanjululwa kwexesha kunye ne-RNA. Itemplate yeRNA inokufumaneka kwimizuzu nje esi-7. Umxube we-5 × ngqo we-RT kunye ne-2 × ngqo qPCR Mix-SYBR reagents ebonelelwe yikiti inokufumana ngokukhawuleza nangokufanelekileyo iziphumo ze-PCR zexesha lokwenyani.
I-5 × i-Direct RT Mix kunye ne-2 × i-Direct QPCR Mix-SYBR ine-inhibitor eyomeleleyo yokunyamezelana, kwaye i-lysate yeesampulu inokusetyenziswa njenge template ye-RT-qPCR ngqo. Le khithi iqulethe i-RNA ekhethekileyo yokuhlangana okuphezulu kwe-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.
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Ukhetho olukhulu lwe-China Real Time Fluorescence Virus Nucleic Acid PCR Test
Ngokusekwe kwikhithi ye-Plant Leaf ngqo ye-PCR, i-DUTP iyasetyenziswa endaweni ye-dTTP, kunye ne-enzyme ye-UNG enokuthomalalisa itemplate ene-DUTP. Ngale ndlela, ukucaciswa kunye nokuchaneka kokukhulisa kuyaqinisekiswa, kwaye ingxaki yongcoliseko lwemveliso yePCR enokuthi yenzeke ngexesha lovavanyo olukhulu lwemfuza kuthintelwe.
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Ixabiso eliphantsi kwiFektri yeChina yeNyukliya yokuKhangela iAcid yeKhompyuter yentsholongwane yeNyukliya
Le mveliso yamkela kuphela ubuchwephesha bekholamu yokuhlanjululwa kwe-DNA kunye nefomula efanelekileyo ye-SDS ye-lysis, enokufumana i-plasmid DNA ekumgangatho ophezulu kwiibhaktheriya ngaphakathi kwemizuzu engama-20.
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I-OEM China China High Sensitivity Nucleic Acid Detection Testing Kits Umatshini wePCR
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu wentsholongwane ye-DNA kunye ne-RNA kwiisampulu ezinje ngeplasma, i-serum, ulwelo olungena selfowuni, kunye nenkcubeko yeseli engaphaya kwamandla. Ikhithi yongeza ngokuthe ngqo i-Linear Acrylamide, enokuthi ngokulula ibambe isixa esincinci se-DNA kunye ne-RNA kwiisampulu. I-DNA / RNA-Ikholamu kuphela enokubopha ngokufanelekileyo iDNA kunye neRNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. IBuffer RW1 kunye neBuffer RW2 inokuqinisekisa ukuba ifumanekile intsholongwane ye-acid engenayo iprotein, nuclease okanye ezinye izinto ezingcolileyo, ezinokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji.
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Ihowuliseyili OEM China Runmei 2021 Ukufika okutsha kwePCR koVavanyo lwezoNyango lweLebhu yeNyukliya
Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu we-RNA yentsholongwane kwiisampulu ezinje ngeplasma, i-serum, ulwelo olungena-cell, kunye nenkcubeko yeseli engaphezulu. Ikhithi yongeza ngokuthe ngqo i-Linear Acrylamide, enokuthi ngokulula ibambe isixa esincinci seRNA kwiisampulu. Ikholamu ye-RNA-kuphela enokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.
Ikhithi yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. I-Buffer viRW1 kunye ne-Buffer viRW2 inokuqinisekisa ukuba ifumanekile intsholongwane ye-acid engenayo iprotein, nuclease okanye ezinye izinto ezingcolileyo, ezinokusetyenziselwa ngokuthe ngqo kuvavanyo lwebhayoloji.
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Quots for China Full Reagents Extractor Nucleic Acid (Generotex)
Ukuncamathisela kumgaqo we "Super High-quality, inkonzo eyonelisayo", besizama ukuba liqabane loshishino elibalaseleyo lakho kwiiQuots zeChina ezizenzekelayo Rea ... -
Ixabiso elifanelekileyo kwi-China Real Time Fluorescence Virus Nucleic Acid PCR Test
Le khithi isebenzisa ubuchwepheshe be-Real PC ye-RTR (rRT-PCR) ekufumaneni umgangatho we-SARS-CoV-2 (i-ORF1ab yemfuza kunye nohlobo lwe-N) yeecicic acid kwi-nasopharyngeal okanye kwi-oropharyngeal swab sampuli, ngendlela ye-multiplex PCR fluorescent probe. ulawulo lwangaphakathi lokuvavanya umgangatho wesampulu, ke ngoko akukho mfuneko yokuhlanjululwa kwe-acid ye-acid, unokugqibezela uvavanyo kwiyure e-1, ngakumbi ifanelekile ukubonwa ngokukhawuleza okukhulu.
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Ukucaciswa okuphezulu kwe-China Nucleic Acid Ukucocwa kwe-Yc-701 Cov Nucleic Acid
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwiisampulu zezicubu zezilwanyana zokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65°C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo weDNA okhutshiweyo unokusetyenziswa njengetemplate yokuphendula kwePCR.
2×PCR KululaTM Umxube (i-UNG) usebenzisa i-DUTP endaweni ye-dTTP kwisiseko se-2×PCR KululaTM Hlanganisa, kwaye wongeza i-UNG enzyme (Uracil-N-glycosylase) enokuthotywa itemplate ene-DUTP ngaxeshanye. Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthoba imveliso yePCR equlathe uracil. I-enzyme ye-UNG ayizukuchaphazeleka kwitemplate engenayo i-uracil, ngaloo ndlela iqinisekisa ubungqangi kunye nokuchaneka kokukhulisa kunye nokuthintela ukubakho kweemveliso ze-PCR zongcoliseko ngexesha lovavanyo olukhulu lofuzo.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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IBakala eliPhezulu leTsholongwane yeNtsholongwane yeNtsholongwane yeNtsholongwane yaseChina, iBhokisi yokuVavanya iNyukliya, iNyukliya yeAcid
I-2X eyi-PCR yokwenene ilulaTM Umxube-SYBR obonelelwe liXesha leXesha le-PCR elilulaTM-SYBR Green I kit yinkqubo entsha yeprimix esebenzisa i-SYBR Green I yeXesha leXesha lokuPhendula kokunyusa i-PCR, enokuphucula kakhulu imveliso ethile kunye nemvakalelo yokuphendula. I-ROX ibonelelwe njengedayi yolawulo lwangaphakathi. Ubungakanani be-fluorescence yale khithi amaxesha angama-3-5 eemveliso ezifanayo, ezinokuba novakalelo ngakumbi kwaye zibonakalise ngokungathandabuzekiyo ukugxila kwethemplate ekujolise kuyo.
2X yokwenene PCR EasyTM Umxube-SYBR uqulethe i-Foregene ekhethekileyo eshushu-yokuqalisa iTaq DNA Polymerase. Xa kuthelekiswa nee-enzyme eziqhelekileyo zeTaq, inezibonelelo zokukhulisa ukusebenza okuphezulu, amandla okukhulisa okomeleleyo kunye nezinga eliphantsi lokungalingani. Inokunciphisa ukukhulisa okungangqalanga kunye nokuphucula ukuchaneka kwePCR.
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Inyukliya Acid
Le khithi isebenzisa ikholamu ye-DNA kuphela enokubopha ngokuthe ngqo i-DNA, Foregene protease kunye nenkqubo ekhethekileyo ye-buffer, eyenza lula ukucocwa kwe-DNA ye-genomic yezityalo. Umgangatho ophezulu we-genomic DNA unokufumaneka kwisithuba semizuzu engama-30, ethintela ukonakaliswa kwe-genomic DNA.
Inwebu yejeli ye-silica ye-DNA esetyenziswe kwikholamu ejikelezayo zizinto ezintsha ezahlukileyo ze-Foregene, ezinokuthi zibambe ngokuthe ngqo kwaye zibophe ngokukodwa kwi-DNA, kunye nokwandisa ukususwa kwe-RNA, iiprotein ezingcolileyo, ion, polysaccharides, polyphenols kunye nezinye izinto eziphilayo.
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Umzi mveliso ngqo kwiChina Nucleic Acid Rapid PCR yoVavanyo lweNtsholongwane yokuVavanya iKhaya ekhaya
Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer ukukhupha ngokukhawuleza i-genomic DNA kwimisila yegundane, iindlebe, izihlunu kunye nezinye iisampulu zethishu yokuphendula kwe-PCR, ke kufanelekile ngokukodwa kuvavanyo lwemfuza olukhulu.
Inkqubo yokukhupha i-genomic DNA kwi-lysis buffer igqityiwe kwisithuba semizuzu eli-10 ukuya kwengama-65 ° C. Azikho ezinye iinkqubo ezinje ngeprotein kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA unokusetyenziswa njengetemplate yokuphendula kwePCR.
I-2 × M1-PCR Easy TM Mix inonyamezelo olomeleleyo kwii-PCR reaction inhibitors, kwaye inokusebenzisa i-lysate yesampulu ukuba ivavanywe njengetemplate yokusebenza ngokufanelekileyo nangokukhulisa okuthile. Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
I-D-Taq DNA polymerase yi-DNA polymerase eyenziwe ngokukodwa yi-Foregene kwi-PCR ngqo. I-D-Taq DNA polymerase inonyamezelo olomeleleyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokukhulisa ngokufanelekileyo ukulandelela izixa ze-DNA kwiinkqubo ezahlukeneyo zokuphendula, kwaye isantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo ngokukodwa ekuphenduleni ngqo kwe-PCR.
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Imveliso eNtsha yaseTshayina IPrueba De Saliva Igg Igm Saliva Test Rapid, iSalva esekwe kuvavanyo lokuchuma, iRina yokuQokelelwa kweNtsholongwane yeRna
Le khithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho ophezulu we-RNA iyonke kwiiseli ezikhule kwi-96, 24, 12, kunye neepleyiti ezingama-6. Ikhithi ibonelela ngekholamu efanelekileyo yokuCoca i-DNA, enokuhlula ngokulula i-supernatant kunye ne-lysate yeseli, ukubopha nokususa i-genomic DNA. Umsebenzi ulula kwaye ugcina ixesha; Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA ngefomula eyahlukileyo. Inani elikhulu leesampulu zinokuqwalaselwa ngaxeshanye.
Yonke le nkqubo ayi-RNase-Simahla, ukuze i-RNA ehlanjululweyo ingahlaziswa; i-Buffer RW1, i-Buffer RW2 ye-buffer yenkqubo yokuhlamba i-RNA engenaziprotheyini, i-DNA, ion kunye nongcoliseko lwekhompawundi.
