Sinamathela kumgaqo "womgangatho kuqala, inkonzo kuqala, ukuphuculwa okuqhubekayo kunye nokuveliswa kwezinto ezintsha ukuhlangabezana nabathengi" kulawulo kunye "neziphene zero, izikhalazo zero" njengenjongo yomgangatho.Ukwenza inkonzo yethu igqibelele, sibonelela ngeemveliso ezinomgangatho olungileyo ngexabiso elifanelekileyo lenkcazo ePhakamileyo yaseChina iNucleic Acid yokuTshintsha iYc-701 Cov Nucleic Acid, Ugxininiso olukhethekileyo malunga nokupakishwa kwezinto ezithengiswayo ukunqanda nawuphi na umonakalo ngexesha lothutho, Inzala eneenkcukacha kwingxelo eluncedo kunye nezicwangciso zabathengi bethu abahlonitshwayo.
Sinamathela kumgaqo "womgangatho kuqala, inkonzo kuqala, ukuphuculwa okuqhubekayo kunye nokuveliswa kwezinto ezintsha ukuhlangabezana nabathengi" kulawulo kunye "neziphene zero, izikhalazo zero" njengenjongo yomgangatho.Ukugqibelela inkonzo yethu, sibonelela ngeemveliso ezinomgangatho olungileyo ngexabiso elifanelekileyoChina Nucleic Acid Extraction System, Nucleic Acid Extraction Instrument, Sisanda ukwandisa isabelo sethu semarike yamazwe ngamazwe ngokusekelwe kwizinto ezisemgangathweni, inkonzo ebalaseleyo, ixabiso elifanelekileyo kunye nokuhanjiswa ngexesha elifanelekileyo.Nceda uqhagamshelane nathi nangaliphi na ixesha ngolwazi oluthe kratya.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi

∎ Imfuno yesampulu incinci, kancinane njenge-5mg inyama yesilwanyana inokuvavanywa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

■ Inkqubo yePCR yokuchasana nongcoliseko 2×PCR Easy^TMUkuxuba (UNG) kuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kwaye iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

Iiparamitha zeKit

Isicelo: iintlobo ezahlukeneyo zezicubu zezilwanyana.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer AL inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

I-Reagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

I-Reagent 6×DNA Loading Buffer ingagcinwa kwi-4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃ .

I-Reagent 2 × PCR Easy^TMUkuxuba (UNG), ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (ukusetyenziswa ngaphakathi kweentsuku ze-10) .Sinamathela kumgaqo "womgangatho wokuqala, inkonzo yokuqala, ukuphuculwa okuqhubekayo kunye nokutsha ukuhlangabezana nabathengi" kulawulo kunye "nesiphako se-zero, izikhalazo zero" njengenjongo yomgangatho.Ukwenza inkonzo yethu igqibelele, sibonelela ngeemveliso ezinomgangatho olungileyo ngexabiso elifanelekileyo lenkcazo ePhakamileyo yaseChina iNucleic Acid yokuTshintsha iYc-701 Cov Nucleic Acid, Ugxininiso olukhethekileyo malunga nokupakishwa kwezinto ezithengiswayo ukunqanda nawuphi na umonakalo ngexesha lothutho, Inzala eneenkcukacha kwingxelo eluncedo kunye nezicwangciso zabathengi bethu abahlonitshwayo.
Ingcaciso ephezuluChina Nucleic Acid Extraction System, Nucleic Acid Extraction Instrument, Sisanda ukwandisa isabelo sethu semarike yamazwe ngamazwe ngokusekelwe kwizinto ezisemgangathweni, inkonzo ebalaseleyo, ixabiso elifanelekileyo kunye nokuhanjiswa ngexesha elifanelekileyo.Nceda uqhagamshelane nathi nangaliphi na ixesha ngolwazi oluthe kratya.