Sinebhongo ngokwaneliseka kwakho kwabathengi kunye nokwamkelwa okubanzi ngenxa yokuzingisa kwethu umgangatho ophezulu ngokulinganayo kwisisombululo kunye nenkonzo yendlela ekhawulezayo yokwahlukaniswa kunye nokucocwa kwe-RNA iyonke kwiiseli, Ukubambelela kwifilosofi yeshishini elincinci 'lomthengi wokuqala, qhubela phambili', siyabamkela ngokunyanisekileyo abathengi abasuka endlwini yakho kunye nokusebenzisana nathi phesheya.
Sinebhongo ngokwaneliseka kwakho kwabathengi kunye nokwamkelwa ngokubanzi ngenxa yokuzingisa kwethu ukufuna umgangatho ophezulu ngokulinganayo kwisisombululo kunye nenkonzoindlela ekhawulezayo yokwahlukaniswa kunye nokucocwa kwe-RNA iyonke kwiiseli ezikhuliswayo, Inkampani yethu isebenza ngomgaqo-siseko wokusebenza "wokusekwe kwintembeko, intsebenziswano edaliweyo, abantu abajolise kubo, baphumelele intsebenziswano".Sinethemba lokuba sinokuba nobudlelwane bobuhlobo kunye nosomashishini abavela kwihlabathi liphela.

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa kwi-4 ℃ inyanga enye emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho). Siye saba nebhongo ngokwaneliseka kwakho kwabathengi kunye nokwamkelwa okubanzi ngenxa yokuzingisa kwethu ukufuna umgangatho ophezulu ngokulinganayo kwisisombululo kunye nenkonzo yexesha elifutshane eliKhokelo kwiChina iminyaka emi-2 yeWarrantying Coating ye-Industrial Ovento yokutshiza iBus encinci, i-Adheraying ye-Industrial Coating kwi-Bus yokutshiza encinci qhubela phambili', siyabamkela ngokunyanisekileyo abathengi abavela endlwini yakho naphesheya kweelwandle ukusebenzisana nathi.
Ixesha elifutshane lokukhokela i-China Powder Coating Booth, i-Industrial Coating, Inkampani yethu isebenza ngomgaqo-nkqubo "wokusekwe kwintembeko, intsebenziswano edaliweyo, abantu abajoliswe kuyo, intsebenziswano yokuphumelela".Sinethemba lokuba sinokuba nobudlelwane bobuhlobo kunye nosomashishini abavela kwihlabathi liphela.