Ngetyala elivakalayo leshishini, inkonzo ebalaseleyo emva kokuthengisa kunye nezixhobo zale mihla zokuvelisa, sizuze igama elihle phakathi kwabathengi bethu kwihlabathi liphela.iseti epheleleyo yee-reagents, Injongo yefemu yethu kufuneka ibe kukubonelela ngezona mpahla zikumgangatho ophezulu ezinexabiso eliphezulu.Besijonge phambili ekwenzeni umbutho nawe!
Ngetyala elivakalayo leshishini, inkonzo ebalaseleyo emva kokuthengisa kunye nezixhobo zale mihla zokuvelisa, sizuze igama elihle phakathi kwabathengi bethu kwihlabathi liphela.iseti epheleleyo yee-reagents, Ngokusekelwe kwiimpahla ezinomgangatho ophezulu, ixabiso elikhuphisanayo, kunye nenkonzo yethu epheleleyo yoluhlu, ngoku siye saqokelela amandla afanelekileyo kunye namava, kwaye siye sazakhela igama elihle kakhulu entsimini.Kunye nophuhliso oluqhubekayo, sizibophelela kungekuphela nje kwishishini lasekhaya laseTshayina kodwa nakwimarike yehlabathi.Ngamana ungashukunyiswa zizinto zethu ezikumgangatho ophezulu kunye nenkonzo enomdla.Masivule isahluko esitsha senzuzo efanayo kwaye siphumelele kabini.

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) Ngetyala leshishini elivakalayo, inkonzo ebalaseleyo emva kokuthengisa kunye nezixhobo zanamhlanje zokuvelisa, sizuze igama elihle phakathi kwabathengi bethu kwihlabathi liphela kwiFactory ngokuthe ngqo iChina Chemical Reagent Bloom Tech Citric Acid CAS 97-92 elungileyo kufuneka ibonelele ngexabiso eliphezulu le-tas 97-92. g.Besijonge phambili ekwenzeni umbutho nawe!
Factory ngqo China Chemical Reagent, Research Chemical, Ngokusekelwe iimpahla ezikumgangatho ophezulu, ixabiso ukhuphiswano, kunye nenkonzo yethu uluhlu olupheleleyo, ngoku siye saqokelela amandla afanelekileyo kunye namava, kwaye siye sazakhela igama elihle kakhulu entsimini.Kunye nophuhliso oluqhubekayo, sizibophelela kungekuphela nje kwishishini lasekhaya laseTshayina kodwa nakwimarike yehlabathi.Ngamana ungashukunyiswa zizinto zethu ezikumgangatho ophezulu kunye nenkonzo enomdla.Masivule isahluko esitsha senzuzo efanayo kwaye siphumelele kabini.