Uphuculo lwethu luxhomekeke kwizixhobo eziphezulu, iitalente ezigqwesileyo kunye nemikhosi yetekhnoloji eyomeleziweyo ngokuqhubekayokhupha i-RNA iyonke, Ukuze ufunde ngakumbi ngezinto esinokukwenzela zona ngokobuqu, thetha nathi nanini na.Sijonge phambili ekuphuhliseni imibutho ebalaseleyo neyexesha elide kunye nawe.
Uphuculo lwethu luxhomekeke kwizixhobo eziphezulu, iitalente ezigqwesileyo kunye nemikhosi yetekhnoloji eyomeleziweyo ngokuqhubekayokhupha i-RNA iyonke, Isebenzisa inkqubo yehlabathi ekhokelayo ekusebenzeni okuthembekileyo, izinga eliphantsi lokusilela, ilungele ukhetho lwabathengi baseArgentina.Inkampani yethu ikwizixeko eziphucukileyo zelizwe, ukugcwala kwezithuthi kulula kakhulu, iimeko ezizodwa zejografi kunye nezoqoqosho.Sisukela imveliso ejolise ebantwini, eyenziwe ngobuchule, sicebisana ngengqondo, sakhe ifilosofi yoshishino eqaqambileyo.Ulawulo olungqongqo lwekhwalithi, inkonzo egqibeleleyo, ixabiso elifanelekileyo eArgentina kukuma kwethu kwisiseko sokhuphiswano.Ukuba kuyimfuneko, wamkelekile ukuba uqhagamshelane nathi ngewebhusayithi yethu okanye ukubonisana ngefowuni, siya kukuvuyela ukukukhonza.

Iinkcukacha

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer Crl1 inokugcinwa kwi-4 ℃ ngenyanga enye emva kongezwe i-2-Hydroxy-1-ethanethiol ixhomekeke kwizinto eziphezulu ze-chinatie ze-China Amino Alid i-Amino Acid i-Suble Suble Suby, Thetha nathi nangaliphi na ixesha.Sijonge phambili ekuphuhliseni imibutho ebalaseleyo neyexesha elide kunye nawe.
IFactory yobuGcisa ye-Amino Acid yaseChina, i-Pharmaceutical, Isebenzisa inkqubo ehamba phambili yehlabathi yokusebenza okuthembekileyo, izinga eliphantsi lokungaphumeleli, lilungele ukhetho lwabathengi baseArgentina.Inkampani yethu ikwizixeko eziphucukileyo zelizwe, ukugcwala kwezithuthi kulula kakhulu, iimeko ezizodwa zejografi kunye nezoqoqosho.Sisukela imveliso ejolise ebantwini, eyenziwe ngobuchule, sicebisana ngengqondo, sakhe ifilosofi yoshishino eqaqambileyo.Ulawulo olungqongqo lwekhwalithi, inkonzo egqibeleleyo, ixabiso elifanelekileyo eArgentina kukuma kwethu kwisiseko sokhuphiswano.Ukuba kuyimfuneko, wamkelekile ukuba uqhagamshelane nathi ngewebhusayithi yethu okanye ukubonisana ngefowuni, siya kukuvuyela ukukukhonza.