Inkqubela phambili yethu ixhomekeke kwiimveliso ezihambele phambili, iitalente ezimangalisayo kunye nemikhosi yetekhnoloji eyomeleziweyo ngokuphindaphindiweyo yoHlolo loMgangatho we-China Utm Virus Kits DNA Test Sampling Tube Oral Swabs Viral Transport Medium Tubes Nasal Swab Vtm Ki, Kwimeko apho ujonge umgangatho ophezulu, ukuhanjiswa okukhawulezayo, eyona nto ingcono kakhulu emva kwenkxaso kunye nexabiso elikhulu kumthengisi e-China, siya kuba sishishini elingcono ixesha elide.
Inkqubela phambili yethu ixhomekeke kwiimveliso eziphambili, iitalente ezimangalisayo kunye nemikhosi yetekhnoloji eyomeleziweyo ngokuphindaphindiweyoChina Vtm Tube, I-Vtm engenanto 10 Ml Tube, Njengomzi-mveliso onamava sikwamkele iodolo elungiselelwe ngokwezifiso kwaye siyenze ifane nomfanekiso wakho okanye isampulu ecacisa ukucaciswa kunye nokupakishwa koyilo lwabathengi.Eyona njongo yenkampani kukuphila inkumbulo eyanelisayo kubo bonke abathengi, kunye nokuseka ubudlelwane beshishini bexesha elide lokuwina.Ngolwazi oluthe vetshe, khumbula ukuqhagamshelana nathi.Kwaye kuluvuyo lwethu ukuba uyathanda ukuba nentlanganiso buqu kwi-ofisi yethu.

Inkcazo

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa kwi-4 ℃ kwinyanga ye-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) .Inkqubela yethu ixhomekeke kwiimveliso eziphuculweyo, iitalente ezimangalisayo kunye nemikhosi yeteknoloji eyomeleziweyo ngokuphindaphindiweyo yoHlolo loMgangatho we-China Utm Virus Kits DNA Test Sampling Tube Oral Swabs Viral Transport Uthutho lweViral uThutho oluPhakathi oluPhakathi lungcono kakhulu emva kokunikezelwa kweVhubhu ePhakathi kwiNasal, kwi-Swab ekhawulezayo yokuhanjiswa kwe-Swab kunye nomthengisi wexabiso elikhulu e-China kuqhagamshelo lweshishini elincinci ixesha elide, siya kuba lolona khetho lwakho lungcono.
Ukuhlolwa koMgangatho weChina Vtm Tube, I-Vtm engenanto 10 Ml Tube, Njengomzi-mveliso onamava sikwamkele iodolo elungiselelwe ngokwezifiso kwaye siyenze ifane nomfanekiso wakho okanye isampulu ecacisa ukucaciswa kunye nokupakishwa koyilo lwabathengi.Eyona njongo yenkampani kukuphila inkumbulo eyanelisayo kubo bonke abathengi, kunye nokuseka ubudlelwane beshishini bexesha elide lokuwina.Ngolwazi oluthe vetshe, khumbula ukuqhagamshelana nathi.Kwaye kuluvuyo lwethu ukuba uyathanda ukuba nentlanganiso buqu kwi-ofisi yethu.