Sihlala siqhuba umoya wethu "wokusungula izinto ezintsha ezizisa ukuqhubela phambili, umgangatho ophezulu wokuqinisekisa ukuphila, ukuthengisa inzuzo yokuthengisa, umlinganiselo wetyala utsalela abathengi base China Factory yase China Baypure High Yield Tissue Genomic DNA Cleanification Kit Reagents kuMntla Melika, Sine-ISO 9001 kunye nesiqinisekiso se-ISO 9001 kwaye siyifanelekele le mveliso okanye inkonzo yethu iminyaka eyi-1 kunye noyilo olugqwesileyo olugqwesileyo kunye noyilo olugqwesileyo oluyi-1 iminyaka kunye noyilo olugqwesileyo lwamava ayi-1 kunye nomgangatho ogqwesileyo we-1. izinga ndlongo.Wamkelekile ukusebenzisana nathi!
Sihlala siqhuba umoya wethu "wokusungula izinto ezintsha ezisa ukuqhubela phambili, umgangatho ophezulu wokuqinisekisa ukuziphilisa, ukuthengisa inzuzo yoLawulo, umlinganiselo wetyala utsala abathengiI-Automatic Nucleic Acid Purification Extractor, China Tissue Genomic DNA Kit, Iintlobo ezininzi zeemveliso ezahlukeneyo ziyafumaneka ukuba ukhethe, unokwenza enye yokuyeka ukuthenga apha.Kwaye iiodolo ezilungiselelweyo zamkelekile.Ishishini lokwenyani kukufumana imeko yokuphumelela, ukuba kunokwenzeka, singathanda ukubonelela ngenkxaso engaphezulu kubathengi.Wamkelekile bonke abathengi abalungileyo banxibelelana iinkcukacha zeemveliso nathi!!

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sihlala siqhuba umoya wethu "wokusungula izinto ezintsha ezizisa ukuqhubela phambili, umgangatho ophezulu wokuqinisekisa ukuphila, ukuthengisa inzuzo yokuthengisa, umlinganiselo wetyala utsalela abathengi base China Factory yase China Baypure High Yield Tissue Genomic DNA Cleanification Kit Reagents kuMntla Melika, Sine-ISO 9001 kunye nesiqinisekiso se-ISO 9001 kwaye siyifanelekele le mveliso okanye inkonzo yethu iminyaka eyi-1 kunye noyilo olugqwesileyo olugqwesileyo kunye noyilo olugqwesileyo oluyi-1 iminyaka kunye noyilo olugqwesileyo lwamava ayi-1 kunye nomgangatho ogqwesileyo we-1. izinga ndlongo.Wamkelekile ukusebenzisana nathi!
China Factory forChina Tissue Genomic DNA Kit, I-Automatic Nucleic Acid Purification Extractor, Iintlobo ezininzi zeemveliso ezahlukeneyo ziyafumaneka ukuba ukhethe, unokwenza enye yokuyeka ukuthenga apha.Kwaye iiodolo ezilungiselelweyo zamkelekile.Ishishini lokwenyani kukufumana imeko yokuphumelela, ukuba kunokwenzeka, singathanda ukubonelela ngenkxaso engaphezulu kubathengi.Wamkelekile bonke abathengi abalungileyo banxibelelana iinkcukacha zeemveliso nathi!!