Enobuchwephesha obugqibeleleyo bolawulo lwezenzululwazi, umgangatho ogqwesileyo kunye nenkolo entle kakhulu, sifumana igama elihle kwaye sahlala kweli candelo leChina iMveliso eNtsha yaseTshayina Baypure Wholesales Tissue Genomic Nucleic Acid Extraction Kit Reagents Asia Market, Ngelixa sisebenzisa itenet "yokholo olusekwe, umthengi kuqala", siyakwamkela abathengi ukuba bafowunele okanye basithumele i-imeyile ukuze sibambisane.
Enobuchule obugqibeleleyo bolawulo lwesayensi, umgangatho obalaseleyo kunye nenkolo entle kakhulu, sifumana igama elihle kwaye sahlala kweli candeloI-Automatic Nucleic Acid Purification Extractor, China Tissue Genomic DNA Kit, Iimveliso zethu zithunyelwa kwihlabathi liphela.Abathengi bethu bahlala benelisekile ngomgangatho wethu othembekileyo, iinkonzo ezijoliswe kubathengi kunye namaxabiso akhuphisanayo.Injongo yethu “kukuqhubeka sifumana ukuthembeka kwakho ngokunikela iinzame zethu ekuphuculeni rhoqo iimveliso kunye neenkonzo zethu ukuze siqinisekise ulwaneliseko lwabasebenzisi bethu bokugqibela, abathengi, abasebenzi, ababoneleli kunye noluntu lwehlabathi esisebenzisana kulo”.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Enobuchwephesha obugqibeleleyo bolawulo lwezenzululwazi, umgangatho ogqwesileyo kunye nenkolo entle kakhulu, sifumana igama elihle kwaye sahlala kweli candelo leChina iMveliso eNtsha yaseTshayina Baypure Wholesales Tissue Genomic Nucleic Acid Extraction Kit Reagents Asia Market, Ngelixa sisebenzisa itenet "yokholo olusekwe, umthengi kuqala", siyakwamkela abathengi ukuba bafowunele okanye basithumele i-imeyile ukuze sibambisane.
China Product NewChina Tissue Genomic DNA Kit, I-Automatic Nucleic Acid Purification Extractor, Iimveliso zethu zithunyelwa kwihlabathi liphela.Abathengi bethu bahlala benelisekile ngomgangatho wethu othembekileyo, iinkonzo ezijoliswe kubathengi kunye namaxabiso akhuphisanayo.Injongo yethu “kukuqhubeka sifumana ukuthembeka kwakho ngokunikela iinzame zethu ekuphuculeni rhoqo iimveliso kunye neenkonzo zethu ukuze siqinisekise ulwaneliseko lwabasebenzisi bethu bokugqibela, abathengi, abasebenzi, ababoneleli kunye noluntu lwehlabathi esisebenzisana kulo”.