Enobuchwephesha obugqibeleleyo besayensi bolawulo, umgangatho ogqwesileyo kunye nenkolo entle kakhulu, sifumana igama elihle kwaye sahlala kweli candelo lobuNgcali baseTshayina eMgangathweni oPhezulu oPhakamileyo weXesha lokwenyani leSiXhobo seLabhoratri yoVavanyo lwePCR, ukuba unomdla kulo naluphi na urhwebo lwethu okanye unqwenela ukuthetha malunga nokuthenga okulungiselelweyo, kufuneka uzive ukhululekile ngokupheleleyo ukuba usibambe.
Enobuchule obugqibeleleyo bolawulo lwesayensi, umgangatho obalaseleyo kunye nenkolo entle kakhulu, sifumana igama elihle kwaye sahlala kweli candeloUkufunyanwa kwe-Acid ye-Nucleic ngokuzenzekelayo, China Rt PCR Izixhobo zokubona, Inkampani yethu, isoloko imalunga nomgangatho njengesiseko senkampani, ifuna uphuhliso ngenqanaba eliphezulu lokuthembeka, ithobela i-iso9000 yomgangatho wolawulo ngokungqongqo, isenza inkampani ekwinqanaba eliphezulu ngomoya wokuthembeka nokuphawula inkqubela phambili kunye nethemba.

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Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides okanye umxholo we-polyphenols.Inika ikholomu ye-DNA-Cleaning enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
■ Ngokukodwa ifanele ukucocwa kwe-RNA kwiisampuli zezityalo ze-polysaccharides kunye ne-polyphenols.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
∎ Isampulu: Izihlunu zezityalo ezitsha okanye ezikhenkcezisiweyo zeepolysaccharides neepolyphenols
∎ Umthamo: 50mg izicubu zezityalo
■ Ubuninzi bomthamo wokubopha i-RNA yekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA epheleleyo kwiisampuli zezicubu zezityalo ezitsha okanye ezikhenkcezisiweyo (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye nomxholo ophezulu we-polysaccharide kunye ne-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ugcino kunye nobomi beShelf

Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kumaqondo obushushu begumbi (15-25℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2–8℃.
I-Buffer PSL1 inokubekwa ku-4℃ kwinyanga e-1 emva kokudibanisa i-β-mercaptoethanol (kuyacetyiswa ukuba uyidibanise ngexesha elifanayo lovavanyo).

Enobuchwephesha obugqibeleleyo besayensi bolawulo, umgangatho ogqwesileyo kunye nenkolo entle kakhulu, sifumana igama elihle kwaye sahlala kweli candelo lobuNgcali baseTshayina eMgangathweni oPhezulu oPhakamileyo weXesha lokwenyani leSiXhobo seLabhoratri yoVavanyo lwePCR, ukuba unomdla kulo naluphi na urhwebo lwethu okanye unqwenela ukuthetha malunga nokuthenga okulungiselelweyo, kufuneka uzive ukhululekile ngokupheleleyo ukuba usibambe.
Ubuchwephesha baseTshayinaChina Rt PCR Izixhobo zokubona, Ukufunyanwa kwe-Acid ye-Nucleic ngokuzenzekelayo, Inkampani yethu, isoloko imalunga nomgangatho njengesiseko senkampani, ifuna uphuhliso ngenqanaba eliphezulu lokuthembeka, ithobela i-iso9000 yomgangatho wolawulo ngokungqongqo, isenza inkampani ekwinqanaba eliphezulu ngomoya wokuthembeka nokuphawula inkqubela phambili kunye nethemba.