Asiyi kuzama kuphela okona kulungileyo ukukunika iimveliso ezibalaseleyo kunye neenkonzo kuye wonke umthengi omnye, kodwa sikulungele ukufumana naziphi na iingcebiso ezinikezelwa ngabathengi bethu ngeXabiso eliKhethekileyo leMagpure Ffpe Rna Kit yeNucleic Acid Isolation & Purification Kit, Ukubonelela ngamathemba ngezixhobo ezinkulu kunye nezisombululo, kwaye rhoqo ukuphuhlisa umatshini omtsha ziinjongo zoshishino lwenkampani yethu.Sijonge phambili ngentsebenziswano yakho.
Asiyi kuzama kuphela okusemandleni ethu ukukunika iimveliso ezigqwesileyo kunye neenkonzo kuye wonke umthengi omnye, kodwa sikulungele ukufumana naziphi na iingcebiso ezinikezelwa ngabathengi bethu.I-China i-Rna / i-DNA Extraction yokuCocwa kunye ne-Nucleic Acid Isolation, Sigxininisa kumgaqo othi "Ityala libe liphambili, abaThengi beyinkosi kwaye uMgangatho ube ngowona ubalaseleyo", sijonge phambili kwintsebenziswano kunye nabo bonke abahlobo basekhaya nakwamanye amazwe kwaye siza kudala ikamva eliqaqambileyo loshishino.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ispin ikholamu kunye nefomulaiphuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo zezilwanyana zezilwanyana kunye nokusebenza okuphezulu.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula kwaye ibhengeze i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate, elula kunye neyonga ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi

Ulwazi lwemveliso

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Amanqaku acatshulweyo

1.UKUBA:18.808:Zheng, Q., Qin, F., Luo, R., et al.I-mRNA-Elayishiweyo iLipid-NjengeNanoparticles yoHlelo oluSiseko seLiver ngokuPhuculwa koYilo oluDityanisiweyo oluPhakathi.Adv.Umsebenzi.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.UKUBA:18.187:Yena X, Hong W, Yang J, et al.I-apoptosis ezenzekelayo yeeseli ekulungiseleleni i-stem cell zonyango zenza i-immunomodulatory iziphumo ngokukhululwa kwe-phosphatidylserine.Ukuhanjiswa kweSignali ekuJoliswe kuyo.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.UKUBA: 17.97:Dai Z, Liu H, Liao J, et al.Ukuguqulwa kwe-N7-Methylguanosine tRNA kwandisa uguqulelo lwe-oncogenic mRNA kwaye ikhuthaza ukuqhubeka kwe-intrahepatic cholangiocarcinoma.Iseli yeMol.2021 Jul 29:S1097-2765 (21) 00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.UKUBA:9.225:Cao X, Shu Y, Chen Y, et al.Ukuguqulwa kwe-M6A ye-Mettl14-Mediated Iququzelela ukuHlaziywa kwesibindi ngokuGcina i-Endoplasmic Reticulum Homeostasis.Iseli Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

RNA isoaltion kits for eminye imithombo yesampuluziyafumaneka:

Iseli, isityalo, intsholongwane, igazi, njl.Asiyi kuzama kuphela okusemandleni ethu ukukunika iimveliso ezigqwesileyo kunye neenkonzo kumthengi ngamnye, kodwa sikulungele ukufumana nayiphi na ingcebiso enikezelwa ngabathengi bethu ngeXabiso eliKhethekileyo leMagpure Ffpe Rna Kit yeNucleic Acid Isolation & Purification Kit, Ukubonelela ngamathemba ngezixhobo ezinkulu kunye nezisombululo, kwaye rhoqo ukuphuhlisa iinjongo ezintsha zenkampani yinkampani yethu.Sijonge phambili ngentsebenziswano yakho.
Ixabiso elikhethekileyo leI-China i-Rna / i-DNA Extraction yokuCocwa kunye ne-Nucleic Acid Isolation, Sigxininisa kumgaqo othi "Ityala libe liphambili, abaThengi beyinkosi kwaye uMgangatho ube ngowona ubalaseleyo", sijonge phambili kwintsebenziswano kunye nabo bonke abahlobo basekhaya nakwamanye amazwe kwaye siza kudala ikamva eliqaqambileyo loshishino.

I-RNA ayikhutshwa okanye izivuno ze-RNA ziphantsi

Kuhlala kukho izinto ezahlukeneyo ezichaphazela ukusebenza kakuhle kokubuyisela, njengale: umxholo we-RNA we-tissue, indlela yokusebenza, umthamo we-lution, njl.

1. Ibhafu ye-ice okanye i-cryogenic (4 ° C) i-centrifugation yenziwa ngexesha lokusebenza.

Isincomo: Sebenza kwiqondo lokushisa (15-25 ° C) kuyo yonke inkqubo, musa ukuhlamba i-ice kunye ne-centrifuge kumaqondo aphantsi.

2. Ukugcinwa kwesampulu engafanelekanga okanye ixesha lokugcina isampuli.

Isindululo: Gcina iisampulu kwi -80 °C okanye umkhenkce kwinitrogen engamanzi kwaye uthintele ukusetyenziswa okuphindaphindiweyo komkhenkce;zama ukusebenzisa izicubu ezitsha okanye iiseli ezikhuliswayo zokutsalwa kwe-RNA.

3. I-lysis yesampuli eyaneleyo.

Isincomo: Xa i-homogenizing tissue, qinisekisa ukuba i-tissue i-homogenized ngokwaneleyo kwaye iiseli zethishu zahlulwe ngokwaneleyo ukucacisa ukukhululwa kwe-RNA.

4. I-eluent ayidityaniswa ngokuchanekileyo.

Ingcebiso: Qinisekisa ukuba i-RNase-Free ddH₂I-O yongezwa kwi-dropwise phakathi kwe-membrane yekholomu yokucoca.

5. Umthamo ochanekileyo we-absolute ethanol awuzange wongezwe kwi-Buffer RL2 okanye kwi-Buffer RW2.

Ingcebiso: Landela imiyalelo, yongeza umthamo ochanekileyo we-ethanol epheleleyo kwi-Buffer RL2 kunye ne-Buffer RW2 kwaye udibanise kakuhle phambi kokusebenzisa ikhithi.

6. Idosi yesampula yezicubu ayifanelekanga.

Isincomo: Sebenzisa i-10-20 mg yezicubu okanye (1-5) × 10⁶iiseli nge-500 μl buffer RL1, njengoko ukusetyenziswa kwethishu ngokugqithisileyo kunokubangela ukutsalwa kwe-RNA okucuthiweyo.

7. Umthamo ongafanelekanga okanye i-elution engaphelelanga.

Isincomo: Umthamo we-elution wekholamu yokucoca yi-50-200 μl;ukuba isiphumo se-elution asanelisi, kuyacetyiswa ukuba kwandiswe ixesha lokubeka ubushushu begumbi emva kokongeza i-RNase-Free ddH efudunyeziweyo.₂O, umz. kwi-5-10 min.

8.Ikholamu yokucoca inentsalela ye-ethanol emva kokuhlamba kwe-Buffer RW2.

Isincomo: Ukuba kukho intsalela ye-ethanol emva kokuhlamba kwe-Buffer RW2, i-tube engenanto ye-centrifugation ye-1min, ixesha le-tube engenanto yokusebenza kwe-centrifugation linganyuswa ukuya kwi-2min, okanye ikholomu yokucoca inokubekwa kwindawo yokushisa ye-5 min ukususa ngokwaneleyo i-ethanol eseleyo.

I-RNA ecocekileyo ithotywa

Umgangatho we-RNA ecocekileyo inxulumene nezinto ezifana nokugcinwa kwesampulu, ukungcoliswa kwe-RNase, kunye nokukhwabanisa, njl.

1. Iisampulu zezicubu azigcinwanga ngexesha.

Isincomo: Ukuba iisampulu zethishu okanye iiseli azisetyenziswanga ngexesha elifanelekileyo emva kokuqokelelwa, ngoko nangoko gcina i-cryopreserve kwi -80 °C okanye initrogen engamanzi.Ukukhupha i-RNA, sebenzisa ithishu esanda kuthathwa okanye isampulu yeseli xa kunokwenzeka.

2. Ukunyibilika okuphindaphindiweyo kweesampuli zethishu.

Isincomo: Xa ugcina iisampulu zethishu, kungcono ukuba uzinqumle zibe ngamaqhekeza amancinci ukuze zigcinwe, kwaye ususe esinye seziqwenga xa uzisebenzisa ukuphepha ukunyibilika okuphindaphindiweyo kwesampulu kunye nokuthotywa kwe-RNA.

3. I-RNase yaziswa okanye ayinxibi iiglavu ezilahlwayo, iimaski, njl njl ngexesha lokusebenza.

Isincomo: Imifuniselo yokutsalwa kwe-RNA yenziwa ngcono kumagumbi okukhohlisa e-RNA kwaye itafile iyacinywa phambi kovavanyo.

Nxiba iiglavu ezilahlwayo kunye nemaski ngexesha lovavanyo lokunciphisa ukuthotywa kwe-RNA okubangelwa kukwaziswa kwe-RNase.

4. Ii-reagents zingcolisekile nge-RNase ngexesha lokusetyenziswa.

Ingcebiso: Faka endaweni entsha yeSilwanyana esipheleleyo se-RNA ye-Isolation Kit ukulungiselela imifuniselo ehambelanayo.

5. Iityhubhu ze-centrifuge, iingcebiso, njl.njl ezisetyenziswa kwi-RNA manipulation zingcolile nge-RNase.

Ingcebiso: Qinisekisa ukuba iityhubhu ze-centrifuge, iingcebiso, iipipette, njl.njl ezisetyenziswa kwi-RNA extraction azinayo yonke i-RNase.

I-RNA esulungekileyo ichaphazela imifuniselo esezantsi

I-RNA ihlanjululwe yikholomu yokucoca, ukuba iiyoni zetyuwa, umxholo weprotheyini mkhulu kakhulu uya kuchaphazela umfuniselo osezantsi, onje: uguqulelo lombhalo, Northern Blot et al.

1. I-RNA ekhutshiweyo ineentsalela ze-ion yetyuwa.

Isincomo: Qinisekisa ukuba umthamo ochanekileyo we-ethanol wongezwe kwi-Buffer RW2 kwaye wenze i-2 ikholamu yokuhlanjululwa kwi-speed ye-centrifugal eboniswe ekusebenzeni;ukuba kukho nayiphi na intsalela ye-ion yetyuwa, shiya ikholomu yokucoca kwi-Buffer RW2 ye-5 min kwiqondo lokushisa elingaphakathi kwaye wenze i-centrifugation ukwandisa ukukhutshwa kwetyuwa.

2. Intsalela ye-Ethanol kwi-RNA elutioned.

Isincomo: Qinisekisa ukuba emva kokuhlamba i-buffer RW2, yenza i-tube engenanto ye-centrifugation operation kwisantya se-centrifugation esiboniswe ukusebenza, ukwandisa ixesha le-tube engenanto ye-centrifugation operation ukuya kwi-2 min ukuba kusekho i-ethanol intsalela, okanye uyishiye kwindawo yokushisa kwe-5 min emva kwe-tube engenanto ye-centrifugation yokwandisa ukususwa kwe-ethanol ye-ethanol.