Silandela imfundiso yolawulo ethi "Umgangatho uyamangalisa, iNkampani iphezulu, igama lelokuqala", kwaye siyakwenza ngokunyanisekileyo kwaye sabelane ngempumelelo nabo bonke abathengi beekhithi zokutsalwa kwe-RNA, Iimveliso zethu kunye nezisombululo zonwabela ukuthandwa okumangalisayo phakathi kwabathengi bethu.Samkela ithemba, imibutho yenkampani kunye nabahlobo abasenyongweni abavela kuzo zonke iindawo zehlabathi ukuba baqhagamshelane nathi kwaye bafune intsebenziswano ukuze bafumane inzuzo efanayo.
Silandela imfundiso yolawulo "Umgangatho uyamangalisa, iNkampani iphezulu, igama lelokuqala", kwaye siyakwenza ngokunyanisekileyo kwaye sabelane ngempumelelo nabo bonke abathengi.Foreegne Stool DNA Rna Extraction Kit, Ukholo lwethu kukunyaniseka kuqala, ngoko ke sibonelela nje ngeempahla ezikumgangatho ophezulu kubathengi bethu.Ngenene sinethemba lokuba sinokuba ngamaqabane oshishino.Sikholelwa ukuba sinokuseka ubudlelwane bexesha elide kunye nomnye.Ungaqhagamshelana nathi ngokukhululekileyo ngolwazi oluthe kratya kunye noluhlu lwamaxabiso lweemveliso zethu kunye nezisombululo !

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ispin ikholamu kunye nefomulaiphuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo zezilwanyana zezilwanyana kunye nokusebenza okuphezulu.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula kwaye ibhengeze i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate, elula kunye neyonga ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi

Ulwazi lwemveliso

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Amanqaku acatshulweyo

1.UKUBA:18.808:Zheng, Q., Qin, F., Luo, R., et al.I-mRNA-Elayishiweyo iLipid-NjengeNanoparticles yoHlelo oluSiseko seLiver ngokuPhuculwa koYilo oluDityanisiweyo oluPhakathi.Adv.Umsebenzi.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.UKUBA:18.187:Yena X, Hong W, Yang J, et al.I-apoptosis ezenzekelayo yeeseli ekulungiseleleni i-stem cell zonyango zenza i-immunomodulatory iziphumo ngokukhululwa kwe-phosphatidylserine.Ukuhanjiswa kweSignali ekuJoliswe kuyo.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.UKUBA: 17.97:Dai Z, Liu H, Liao J, et al.Ukuguqulwa kwe-N7-Methylguanosine tRNA kwandisa uguqulelo lwe-oncogenic mRNA kwaye ikhuthaza ukuqhubeka kwe-intrahepatic cholangiocarcinoma.Iseli yeMol.2021 Jul 29:S1097-2765 (21) 00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.UKUBA:9.225:Cao X, Shu Y, Chen Y, et al.Ukuguqulwa kwe-M6A ye-Mettl14-Mediated Iququzelela ukuHlaziywa kwesibindi ngokuGcina i-Endoplasmic Reticulum Homeostasis.Iseli Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

RNA isoaltion kits for eminye imithombo yesampuluziyafumaneka:

Iseli, isityalo, intsholongwane, igazi, njl.Silandela imfundiso yolawulo "Umgangatho uyamangalisa, iNkampani iphezulu, iGama lokuqala", kwaye siya kudala ngokunyanisekileyo kwaye sabelane ngempumelelo nabo bonke abathengi be-T181H yaseChina, iimveliso kunye nezisombululo zethu ziyakuvuyela ukuthandwa okumangalisayo phakathi kwabathengi bethu.Samkela ithemba, imibutho yenkampani kunye nabahlobo abasenyongweni abavela kuzo zonke iindawo zehlabathi ukuba baqhagamshelane nathi kwaye bafune intsebenziswano ukuze bafumane inzuzo efanayo.
I-China Wholesale Foregene Stool DNA Rna Extraction Kit, ukholo lwethu kukunyaniseka kuqala, ngoko ke sibonelela ngeempahla ezikumgangatho ophezulu kubathengi bethu.Ngenene sinethemba lokuba sinokuba ngamaqabane oshishino.Sikholelwa ukuba sinokuseka ubudlelwane bexesha elide kunye nomnye.Ungaqhagamshelana nathi ngokukhululekileyo ngolwazi oluthe kratya kunye noluhlu lwamaxabiso lweemveliso zethu kunye nezisombululo !

I-RNA ayikhutshwa okanye izivuno ze-RNA ziphantsi

Kuhlala kukho izinto ezahlukeneyo ezichaphazela ukusebenza kakuhle kokubuyisela, njengale: umxholo we-RNA we-tissue, indlela yokusebenza, umthamo we-lution, njl.

1. Ibhafu ye-ice okanye i-cryogenic (4 ° C) i-centrifugation yenziwa ngexesha lokusebenza.

Isincomo: Sebenza kwiqondo lokushisa (15-25 ° C) kuyo yonke inkqubo, musa ukuhlamba i-ice kunye ne-centrifuge kumaqondo aphantsi.

2. Ukugcinwa kwesampulu engafanelekanga okanye ixesha lokugcina isampuli.

Isindululo: Gcina iisampulu kwi -80 °C okanye umkhenkce kwinitrogen engamanzi kwaye uthintele ukusetyenziswa okuphindaphindiweyo komkhenkce;zama ukusebenzisa izicubu ezitsha okanye iiseli ezikhuliswayo zokutsalwa kwe-RNA.

3. I-lysis yesampuli eyaneleyo.

Isincomo: Xa i-homogenizing tissue, qinisekisa ukuba i-tissue i-homogenized ngokwaneleyo kwaye iiseli zethishu zahlulwe ngokwaneleyo ukucacisa ukukhululwa kwe-RNA.

4. I-eluent ayidityaniswa ngokuchanekileyo.

Ingcebiso: Qinisekisa ukuba i-RNase-Free ddH₂I-O yongezwa kwi-dropwise phakathi kwe-membrane yekholomu yokucoca.

5. Umthamo ochanekileyo we-absolute ethanol awuzange wongezwe kwi-Buffer RL2 okanye kwi-Buffer RW2.

Ingcebiso: Landela imiyalelo, yongeza umthamo ochanekileyo we-ethanol epheleleyo kwi-Buffer RL2 kunye ne-Buffer RW2 kwaye udibanise kakuhle phambi kokusebenzisa ikhithi.

6. Idosi yesampula yezicubu ayifanelekanga.

Isincomo: Sebenzisa i-10-20 mg yezicubu okanye (1-5) × 10⁶iiseli nge-500 μl buffer RL1, njengoko ukusetyenziswa kwethishu ngokugqithisileyo kunokubangela ukutsalwa kwe-RNA okucuthiweyo.

7. Umthamo ongafanelekanga okanye i-elution engaphelelanga.

Isincomo: Umthamo we-elution wekholamu yokucoca yi-50-200 μl;ukuba isiphumo se-elution asanelisi, kuyacetyiswa ukuba kwandiswe ixesha lokubeka ubushushu begumbi emva kokongeza i-RNase-Free ddH efudunyeziweyo.₂O, umz. kwi-5-10 min.

8.Ikholamu yokucoca inentsalela ye-ethanol emva kokuhlamba kwe-Buffer RW2.

Isincomo: Ukuba kukho intsalela ye-ethanol emva kokuhlamba kwe-Buffer RW2, i-tube engenanto ye-centrifugation ye-1min, ixesha le-tube engenanto yokusebenza kwe-centrifugation linganyuswa ukuya kwi-2min, okanye ikholomu yokucoca inokubekwa kwindawo yokushisa ye-5 min ukususa ngokwaneleyo i-ethanol eseleyo.

I-RNA ecocekileyo ithotywa

Umgangatho we-RNA ecocekileyo inxulumene nezinto ezifana nokugcinwa kwesampulu, ukungcoliswa kwe-RNase, kunye nokukhwabanisa, njl.

1. Iisampulu zezicubu azigcinwanga ngexesha.

Isincomo: Ukuba iisampulu zethishu okanye iiseli azisetyenziswanga ngexesha elifanelekileyo emva kokuqokelelwa, ngoko nangoko gcina i-cryopreserve kwi -80 °C okanye initrogen engamanzi.Ukukhupha i-RNA, sebenzisa ithishu esanda kuthathwa okanye isampulu yeseli xa kunokwenzeka.

2. Ukunyibilika okuphindaphindiweyo kweesampuli zethishu.

Isincomo: Xa ugcina iisampulu zethishu, kungcono ukuba uzinqumle zibe ngamaqhekeza amancinci ukuze zigcinwe, kwaye ususe esinye seziqwenga xa uzisebenzisa ukuphepha ukunyibilika okuphindaphindiweyo kwesampulu kunye nokuthotywa kwe-RNA.

3. I-RNase yaziswa okanye ayinxibi iiglavu ezilahlwayo, iimaski, njl njl ngexesha lokusebenza.

Isincomo: Imifuniselo yokutsalwa kwe-RNA yenziwa ngcono kumagumbi okukhohlisa e-RNA kwaye itafile iyacinywa phambi kovavanyo.

Nxiba iiglavu ezilahlwayo kunye nemaski ngexesha lovavanyo lokunciphisa ukuthotywa kwe-RNA okubangelwa kukwaziswa kwe-RNase.

4. Ii-reagents zingcolisekile nge-RNase ngexesha lokusetyenziswa.

Ingcebiso: Faka endaweni entsha yeSilwanyana esipheleleyo se-RNA ye-Isolation Kit ukulungiselela imifuniselo ehambelanayo.

5. Iityhubhu ze-centrifuge, iingcebiso, njl.njl ezisetyenziswa kwi-RNA manipulation zingcolile nge-RNase.

Ingcebiso: Qinisekisa ukuba iityhubhu ze-centrifuge, iingcebiso, iipipette, njl.njl ezisetyenziswa kwi-RNA extraction azinayo yonke i-RNase.

I-RNA esulungekileyo ichaphazela imifuniselo esezantsi

I-RNA ihlanjululwe yikholomu yokucoca, ukuba iiyoni zetyuwa, umxholo weprotheyini mkhulu kakhulu uya kuchaphazela umfuniselo osezantsi, onje: uguqulelo lombhalo, Northern Blot et al.

1. I-RNA ekhutshiweyo ineentsalela ze-ion yetyuwa.

Isincomo: Qinisekisa ukuba umthamo ochanekileyo we-ethanol wongezwe kwi-Buffer RW2 kwaye wenze i-2 ikholamu yokuhlanjululwa kwi-speed ye-centrifugal eboniswe ekusebenzeni;ukuba kukho nayiphi na intsalela ye-ion yetyuwa, shiya ikholomu yokucoca kwi-Buffer RW2 ye-5 min kwiqondo lokushisa elingaphakathi kwaye wenze i-centrifugation ukwandisa ukukhutshwa kwetyuwa.

2. Intsalela ye-Ethanol kwi-RNA elutioned.

Isincomo: Qinisekisa ukuba emva kokuhlamba i-buffer RW2, yenza i-tube engenanto ye-centrifugation operation kwisantya se-centrifugation esiboniswe ukusebenza, ukwandisa ixesha le-tube engenanto ye-centrifugation operation ukuya kwi-2 min ukuba kusekho i-ethanol intsalela, okanye uyishiye kwindawo yokushisa kwe-5 min emva kwe-tube engenanto ye-centrifugation yokwandisa ukususwa kwe-ethanol ye-ethanol.