Sisebenzisa inkqubo yolawulo lomgangatho ophezulu wenzululwazi, umgangatho ophezulu ophezulu kunye nokholo oluphezulu, sifumana igama elihle kwaye sahlala kweli shishiniUmgangatho ophezulu we-RNA, Umgaqo wethu "ngamaxabiso afanelekileyo, ixesha lemveliso enoqoqosho kunye nenkonzo egqwesileyo" Sinethemba lokusebenzisana nabathengi abaninzi ngakumbi ukuze siphucule kunye neenzuzo.
Sisebenzisa inkqubo yolawulo lomgangatho ophezulu wenzululwazi, umgangatho ophezulu ophezulu kunye nokholo oluphezulu, sifumana igama elihle kwaye sahlala kweli shishiniUmgangatho ophezulu we-RNA, Inkampani yethu ijonga "amaxabiso afanelekileyo, umgangatho ophezulu, ixesha lokuvelisa elisebenzayo kunye nenkonzo elungileyo emva kokuthengisa" njengesiseko sethu.Sinethemba lokusebenzisana nabathengi abaninzi kuphuhliso olufanayo kunye neenzuzo kwixesha elizayo.Wamkelekile ukuba uqhagamshelane nathi.

Iinkcukacha

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Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa kwi-4 ℃ inyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho). Ukusebenzisa inkqubo yolawulo oluphezulu lwesayensi, umgangatho ophezulu ophezulu kunye nokholo oluphezulu, sifumana igama elihle kwaye sahlala kweli shishini loMgangatho oPhezulu we-China Longyu Ukubonelela ngoMgangatho othembekileyo weNomilin, i-tenet yethu yi "imveliso yexabiso elilungileyo kunye nexesha elingcono kakhulu lemveliso yoqoqosho" uphuculo kunye neenzuzo.
Umgangatho oPhezulu we-China Nomilin, i-Solvent Extraction, Inkampani yethu ijonga "amaxabiso afanelekileyo, umgangatho ophezulu, ixesha elisebenzayo lokuvelisa kunye nenkonzo efanelekileyo emva kokuthengisa" njengesiseko sethu.Sinethemba lokusebenzisana nabathengi abaninzi kuphuhliso olufanayo kunye neenzuzo kwixesha elizayo.Wamkelekile ukuba uqhagamshelane nathi.