Ukubambelela kwimbono "yokwenza iimveliso eziphezulu kuluhlu kunye nomvuzo wamaqabane kunye nabantu namhlanje abavela kwihlabathi lonke", sihlala sibeka umnqweno wabathengi kwindawo yokuqala ye-Wholesale Ixabiso laseChina i-China Best Quality 1% 1-Deoxynojirimycin Mulberry Leaf Extract, Samkela abathengi abatsha nabangaphambili abavela kuzo zonke iindlela zokuphila ukuze baqhagamshelane nathi kunye nobudlelwane bezoshishino ixesha elide!
Ukubambelela kwimbono "yokwenza iimveliso eziphezulu kuluhlu kunye nokufumana amaqabane nabantu namhlanje abavela kwihlabathi liphela", sihlala sibeka umnqweno wabathengi kwindawo yokuqala.China Mulberry Leaf Extract, Ukutsalwa kwesinyibilikisi, Ngophuhliso kunye nokwandiswa kwabathengi abaninzi kumazwe aphesheya, ngoku siye saseka ubudlelwane bentsebenziswano kunye neempawu ezininzi ezinkulu.Sinomzi-mveliso wethu kwaye sinemizi-mveliso emininzi ethembekileyo nesebenzisana kakuhle ebaleni.Ukuthobela “umgangatho kuqala, umthengi kuqala, Sibonelela ngomgangatho ophezulu, iimveliso ezinexabiso eliphantsi kunye nezisombululo kunye nenkonzo yodidi lokuqala kubathengi.Sithemba ngokunyanisekileyo ukuseka ubudlelwane beshishini kunye nabathengi abavela kwihlabathi liphela ngesiseko somgangatho, bazuze ngokufanayo.Siyazamkela iiprojekthi ze-OEM kunye noyilo.

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Ukubambelela kwimbono "yokwenza iimveliso eziphezulu kuluhlu kunye nomvuzo wamaqabane kunye nabantu namhlanje abavela kwihlabathi lonke", sihlala sibeka umnqweno wabathengi kwindawo yokuqala ye-Wholesale Ixabiso laseChina i-China Best Quality 1% 1-Deoxynojirimycin Mulberry Leaf Extract, Samkela abathengi abatsha nabangaphambili abavela kuzo zonke iindlela zokuphila ukuze baqhagamshelane nathi kunye nobudlelwane bezoshishino ixesha elide!
Ixabiso leWholeyile yaseTshayinaChina Mulberry Leaf Extract, Ukutsalwa kwesinyibilikisi, Ngophuhliso kunye nokwandiswa kwabathengi abaninzi kumazwe aphesheya, ngoku siye saseka ubudlelwane bentsebenziswano kunye neempawu ezininzi ezinkulu.Sinomzi-mveliso wethu kwaye sinemizi-mveliso emininzi ethembekileyo nesebenzisana kakuhle ebaleni.Ukuthobela “umgangatho kuqala, umthengi kuqala, Sibonelela ngomgangatho ophezulu, iimveliso ezinexabiso eliphantsi kunye nezisombululo kunye nenkonzo yodidi lokuqala kubathengi.Sithemba ngokunyanisekileyo ukuseka ubudlelwane beshishini kunye nabathengi abavela kwihlabathi liphela ngesiseko somgangatho, bazuze ngokufanayo.Siyazamkela iiprojekthi ze-OEM kunye noyilo.