Sigcina ukuphuculwa kunye nokugqibelela izinto zethu kunye nokulungiswa.Kwangaxeshanye, senza ngenkuthalo ukwenza uphando kunye nenkqubela-phambili yexabiso leDiscount China Bioer Bsc70 Simplyp Animal Pathogens DNA/Rna Extraction Kit Manufacturer Production, Samkela ngokufudumeleyo yonke imibuzo yembono evela ekhaya naphesheya ukusebenzisana nathi, kwaye sijonge phambili kwimbalelwano yakho.
Sigcina ukuphucula kunye nokugqibelela izinto zethu kunye nokulungiswa.Kwangaxeshanye, sisebenza ngenkuthalo ukwenza uphando kunye nenkqubela phambiliChina Animal DNA / Rna, Amaso Magnetic, Ngenkxaso yeengcali zethu ezinamava kakhulu, senza kwaye sinikezela ngeempahla ezisemgangathweni.Ezi zivavanywe umgangatho ngamaxesha ahlukeneyo ukuqinisekisa ukuba kuphela uluhlu olungenasiphako olunikezelwa kubathengi, sikwalungisa uluhlu njengoko kufuneka sibe nabathengi ukuhlangabezana neemfuno zabathengi.

Iinkcukacha

50 Preps, 200 Preps

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sigcina ukuphuculwa kunye nokugqibelela izinto zethu kunye nokulungiswa.Kwangaxeshanye, senza ngenkuthalo ukwenza uphando kunye nenkqubela-phambili yexabiso leDiscount China Bioer Bsc70 Simplyp Animal Pathogens DNA/Rna Extraction Kit Manufacturer Production, Samkela ngokufudumeleyo yonke imibuzo yembono evela ekhaya naphesheya ukusebenzisana nathi, kwaye sijonge phambili kwimbalelwano yakho.
Ixabiso lesaphuleloChina Animal DNA / Rna, Amaso Magnetic, Ngenkxaso yeengcali zethu ezinamava kakhulu, senza kwaye sinikezela ngeempahla ezisemgangathweni.Ezi zivavanywe umgangatho ngamaxesha ahlukeneyo ukuqinisekisa ukuba kuphela uluhlu olungenasiphako olunikezelwa kubathengi, sikwalungisa uluhlu njengoko kufuneka sibe nabathengi ukuhlangabezana neemfuno zabathengi.