Ikhomishini yethu kufuneka ibe kukukhonza abasebenzisi bethu bokugqibela kunye nabathengi ngomgangatho ophezulu ogqwesileyo kunye nokhuphiswano lweemveliso zedijithali kunye nezisombululoPCR Reagent, Samkela ngokunyanisekileyo amahlakani amabini aphesheya kunye namashishini asekhaya, kwaye sinethemba lokusebenzisana nawe kwixesha elide!
Ikhomishini yethu kufuneka ibe kukukhonza abasebenzisi bethu bokugqibela kunye nabathengi ngomgangatho ophezulu ogqwesileyo kunye nokhuphiswano lweemveliso zedijithali kunye nezisombululoPCR Reagent, Phantse iminyaka engama-30 yamava kushishino, siye sazithemba kwinkonzo ekumgangatho ophezulu, umgangatho kunye nonikezelo.Samkela ngokufudumeleyo abathengi abavela kwihlabathi liphela ukusebenzisana nenkampani yethu kuphuhliso olufanayo.

Iinkcukacha

5ml, 10ml, 40ml

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukuthembeka okuphezulu: amaxesha ama-6 kune-enzyme yeTaq eqhelekileyo;

■ Isantya sokukhulisa ngokukhawuleza : isantya sokukhulisa yi-5-10sec/kb, ephindwe ka-6-12 kune-enzyme ye-Taq eqhelekileyo;

■ Ukuguquguquka okuthe kratya kwetemplate: Inokukhulisa ngokufanelekileyo iitemplate ezahlukeneyo ze-DNA ezintsonkothileyo eziphezuluUmxholo weGC;

■ Ubuchule obuphezulu bokwandisa: imijikelo esezantsi yokwandisa kune-enzyme yeTaq eqhelekileyo;

■ Ukunyamezela kokusingqongileyo kunamandla: ibekwe kwi-37 ° C ngeveki, igcina umsebenzi ongaphezulu kwe-90%;

■ Inomsebenzi we-5′ →3′ DNA polymerase kunye no-5′→3′ umsebenzi we-exonuclease, ngaphandle kwe-3′ →5′ umsebenzi we-exonuclease.

■ Ikiti ixutywe kwangaphambili kunye ne-Electrophoresis Loading Buffer, emva kokuba i-PCR igqityiwe, isampuli inokulayishwa ngokuthe ngqo kwi-electrophoresis;ukusebenza kulula kwaye kulula.

Isicelo seKit

Ukwandiswa kwePCR yamaqhekeza eDNA, ukuleyibhelishwa kweDNA, ulandelelwano lweDNA, PCR kunye nomsila

Ugcino kunye nobomi beShelf

Inokugcinwa ixesha elide kwi -20 ° C;ukusetyenziswa rhoqo, inokugcinwa kwi-4 ° C ixesha elifutshane.I-2 × Real PCR Easy TM Mix-SYBR enikezelwa yi-Real Time PCR Easy TM-SYBR I-Green I kit yinkqubo entsha ye-premix esebenzisa i-SYBR Green I kwi-Real Time ye-PCR yokuphendula i-amplification, enokuphucula kakhulu imveliso ethile kunye novelwano lokuphendula.I-ROX inikezelwa njengedayi yolawulo lwangaphakathi.Ukuqina kwe-fluorescence yale khithi ngama-3-5 amaxesha eemveliso ezifanayo, ezinokuthi zibe novakalelo kwaye zibonise ngokucacileyo ukuxinwa kwe-template ye-DNA ekujoliswe kuyo.
I-2 × I-PCR yokwenene i-Easy TM Mix-SYBR iqulethe i-Foregene ekhethekileyo eshushu-isiqalo se-Taq DNA Polymerase.Xa kuthelekiswa nee-enzymes ze-Taq eziqhelekileyo, ineengenelo zokusebenza kwe-amplification ephezulu, isakhono esithile esinamandla sokukhulisa kunye nesantya esisezantsi sokungahambelani.Inokunciphisa ukukhuliswa okungangqaliyo kunye nokuphucula ukuchaneka kwe-PCR.