Sihlala sikholelwa ukuba umlingiswa womntu uthatha isigqibo somgangatho weemveliso, iinkcukacha zithatha isigqibo somgangatho weemveliso, kunye nomoya weqela OLUYIKWENENE, OLUSEBENZAYO KUNYE NONCWAYO weqela laseTshayina lexabiso eliSebenzayo lomvelisi iWholesales Universal DNA Rna Isolation Kit Reagents, Siyabamkela abathengi, imibutho yoshishino kunye namaqabane kuwo onke amacandelo ehlabathi ukuze adibane nathi kwaye aqhagamshelane nathi.
Sihlala sikholelwa ukuba umlingiswa womntu uthatha isigqibo somgangatho weemveliso, iinkcukacha zithatha isigqibo somgangatho weemveliso, kunye nomoya onyanisekileyo, osebenzayo kunye neqela elitsha.China Universal Rna DNA Reagents, Ngenxa yokurhweba kunye neenkonzo zethu ezilungileyo, ngoku sifumene igama elihle kunye nokuthembeka kubathengi basekhaya nabamazwe ngamazwe.Ukuba ufuna ulwazi oluthe kratya kwaye unomdla kuyo nayiphi na into yethu, qiniseka ukuba uziva ukhululekile ukuqhagamshelana nathi.Sijonge phambili ekubeni ngumthengisi wakho kwixesha elizayo elingekude.

Iinkcazelo Kits

50 Preps, 200 Preps

Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwizicubu zezilwanyana ezahlukeneyo kunye nokusebenza kakuhle.Inika iKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukuhlula ngokulula kwaye ibhengeze i-DNA ye-genomic ukusuka kwi-supernatant kunye ne-tissue lysate, elula kunye nokugcina ixesha;IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA kwaye inokucutshungulwa ngaxeshanye ngefomula eyodwa Iisampuli ezininzi.

Yonke inkqubo i-RNase-Free, ukuze i-RNA ekhutshiweyo ingathotywanga;I-Buffer RW1, i-Buffer RW2 inkqubo yokuhlamba i-buffer, ukwenzela ukuba i-RNA efunyenweyo ayinayo iprotheni, i-DNA, i-ion, kunye nongcoliseko lwe-organic compound.

Amacandelo ekhithi:

Iimpawu&izinto eziluncedo

■ Akukho mfuneko yakuzikhathaza ngokuthotywa kweRNA;yonke inkqubo i-RNase-Free
■ Ukususa ngokufanelekileyo i-DNA-usebenzisa i-DNA-Cleaning Column
■ Susa i-DNA ngaphandle kokufaka i-DNase
■ Ilula-yonke imisebenzi igqityezelwa kubushushu begumbi
■ Ukukhawuleza -ukusebenza kunokugqitywa kwimizuzu engama-30
∎ Ekhuselekileyo-akukho zixhobo zokuvuselela izinto eziphilayo ezifunekayo
■ Ukucoceka okuphezulu -OD260/280≈1.8-2.1

Isicelo seKit

Ilungele ukutsalwa kunye nokucocwa kwe-RNA iyonke ukusuka kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli ezikhulisiwe.

Iiparamitha zemveliso

■ Izicelo ezisezantsi: i-first-strand cDNA synthesis, RT-PCR, i-molecular cloning, i-Northern Blot, njl.
■ Iisampulu: izihlunu zezilwanyana, iiseli ezikhuliswayo
■ Umthamo: Izicubu 10-20mg, Iiseli(2-5)×10⁶
■ Ubuninzi be-DNA yokubopha umthamo wekholomu yokucoca: 80 μg
■ Umthamo we-Elution: 50-200 μl

Ukuhamba komsebenzi

Umzobo

Animal Total RNA Isolation Kit baphathwa 20mg
Iisampulu ezintsha zemouse, thatha i-5% esulungekileyo iyonke i-RNA 1% ye-agar

Glycogel electrophoresis
1: Udakada 2: Izintso
3: Isibindi 4: Intliziyo

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezingama-24 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide.I-Buffer RL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-β-mercaptoethanol (ukhetho).

Sihlala sikholelwa ukuba umlingiswa womntu ugqiba umgangatho weemveliso, iinkcukacha zithatha isigqibo somgangatho weemveliso, kunye nomoya weqela OLUYIKWENENE, OLUSEBENZAYO KUNYE NOYINYANGA LWENKULUMO yoKhetho olukhulu lwaseTshayina lweendleko eziSebenzayo zoMvelisi we-Wholesales Universal DNA Rna Isolation Kit Reagents, Samkela abathengi, imibutho yoshishino kunye namaqabane kuwo onke amacandelo onxibelelwano kunye nokufuna intsebenziswano kunye nehlabathi.
Ukhetho olukhulu lweChina Universal Rna DNA Reagents, Ukuhlanjululwa kwe-Acid ye-Automatic Nucleic Acid, Ngenxa yokuthengisa kunye neenkonzo zethu ezilungileyo, ngoku sifumene igama elihle kunye nokuthembeka kubathengi basekhaya nabamazwe ngamazwe.Ukuba ufuna ulwazi oluthe kratya kwaye unomdla kuyo nayiphi na into yethu, qiniseka ukuba uziva ukhululekile ukuqhagamshelana nathi.Sijonge phambili ekubeni ngumthengisi wakho kwixesha elizayo elingekude.