Sinale motto engqondweni, sijike saba yenye yezona zinto zinokwenzeka kwezobuchwepheshe, ezonga imali, kunye nabavelisi abakhuphisana ngamaxabiso kuMgangatho oPhezulu.China Nucleic Acid Extraction Reagent, Izixhobo zoVavanyo lwe-Rna Extraction ye-Nucleic Acid Extraction Reagent, Wamkelekile ukuba ngokuqinisekileyo ube yinxalenye yethu ecaleni komnye ukuze wenze umbutho wakho ube lula.Besiqhelekile ukuba liqabane lakho elikhulu xa ufuna ukuba neshishini lakho elincinci.
Sinale motto engqondweni, sijike saba yenye yezona zinto zinokwenzeka kwezobuchwepheshe, ezonga imali, kunye nabavelisi abakhuphisana ngamaxabiso ezinto.China Nucleic Acid Extraction Reagent, Indlela ye-Magnetic Bead, Siza kwenza konke okusemandleni ethu ukusebenzisana kunye nokwaneliseka ngawe sithembele kumgangatho ophezulu kunye nexabiso elikhuphisanayo kwaye emva kwenkonzo, ngokunyanisekileyo sijonge phambili ukusebenzisana nawe kunye nokwenza impumelelo kwixesha elizayo!

Inkcazo

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Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 ingagcinwa kwi-4 ℃ inyanga enye emva kokongeza i-2-hydroxy-1-ethanethiol(ukhetho). Unale motto engqondweni, sijike saba yenye yezona zinto zinokwenzeka zobuchwepheshe zintsha, zingabizi kakhulu, kunye nabavelisi abakhuphisana ngamaxabiso kuMgangatho oPhezulu.China Nucleic Acid Extraction Reagent, Izixhobo zoVavanyo lwe-Rna Extraction ye-Nucleic Acid Extraction Reagent, Wamkelekile ukuba ngokuqinisekileyo ube yinxalenye yethu ecaleni komnye ukuze wenze umbutho wakho ube lula.Besiqhelekile ukuba liqabane lakho elikhulu xa ufuna ukuba neshishini lakho elincinci.
Umgangatho ophezulu waseTshayina weNucleic Acid Extraction Reagent,Indlela ye-Magnetic Bead, Siza kwenza konke okusemandleni ethu ukusebenzisana kunye nokwaneliseka ngawe sithembele kumgangatho ophezulu kunye nexabiso elikhuphisanayo kwaye emva kwenkonzo, ngokunyanisekileyo sijonge phambili ukusebenzisana nawe kunye nokwenza impumelelo kwixesha elizayo!