"Umgangatho wokuqala, ukunyaniseka njengesiseko, uncedo olunyanisekileyo kunye nenzuzo efanayo" ngumbono wethu, njengendlela yokuvelisa ngokuqhubekayo kwaye silandele ukugqwesa kwi-Wholesale ye-Wholesale yase-China Ixabiso eliSebenzayo loMenzi we-Wholesales Universal DNA Rna Isolation Kit Reagents, Uphenjelelwe ukusuka kwindawo yentengiso ekhawulezayo yokwakha ukutya okukhawulezayo kunye nesiselo esiphuphumayo sihamba phambili kunye neziphumo ezidliwayo kunye neziselo ezisetyenziswayo. kunye.
"Umgangatho wokuqala, ukunyaniseka njengesiseko, uncedo olunyanisekileyo kunye nenzuzo efanayo" ngumbono wethu, njengendlela yokuvelisa ngokuqhubekayo kunye nokusukela ukugqwesaUkuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, China Universal Rna DNA Reagents, Inkampani yethu ngoku inamasebe amaninzi, kwaye kukho abasebenzi abangaphezu kwama-20 kwinkampani yethu.Siye saseka ivenkile yokuthengisa, igumbi lokubonisa, kunye nendawo yokugcina imveliso.Okwangoku, sibhalise igama lethu.Siye saqinisa uhlolo lomgangatho wemveliso.

Inkcazo

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Le kit isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiiseli ezikhuliswe kwi-96, i-24, i-12, kunye ne-6-well-plates.

Ikiti ibonelela ngeKholamu ye-DNA-Cleaning esebenzayo, ekwazi ukwahlula ngokulula i-supernatant kunye ne-cell lysate, ibophe kwaye isuse i-genomic DNA.Umsebenzi ulula kwaye wonga ixesha.

IKholamu ye-RNA kuphela inokubophelela ngokufanelekileyo i-RNA ngefomula ekhethekileyo.Inani elikhulu leisampulu linokucutshungulwa ngaxeshanye.

Amacandelo ekhithi

*Nceda unxibe iiglavu kwaye uthathe amanyathelo okukhusela ngexesha lomsebenzi njengoko i-Buffer cRL1 kunye ne-Buffer RW1 ziqulethe iityuwa ze-chaotropic ezicaphukisayo.

Iimpawu&izinto eziluncedo

■ Yonke le nkqubo iqhutywa kubushushu begumbi (15-25℃), ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Ikhithi iyonke i-RNase-Free, akukho mfuneko yakuzikhathaza ngokonakala kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-DNA ye-genomic ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa ngemizuzu eyi-11.
■ Ukhuseleko: Akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: I-RNA ecociweyo yeyobunyulu obuphezulu, ayinaprotheyini kunye nobunye ukungcola, kwaye inokumelana nemifuniselo eyahlukeneyo elandelayo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-RNA epheleleyo kwiiseli ezikhuliswe kwii-96, 24, 12, kunye ne-6-well-plates.

Ukuhamba komsebenzi

Umzobo

Umzobo webhetri yejeli ye-agarose yeCell Total RNA Isolation Kit yaphatha amanani angasentla ahlukeneyo eeseli, 20μl umthamo we-elution, thatha i-2μl esulungekisiweyo iyonke ye-RNA 1%.

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) okanye 2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer cRL1 inokugcinwa kwi-4 ℃ inyanga enye emva kokongeza i-2-hydroxy-1-ethanethiol(ukhetho)."Umgangatho wokuqala, Ukunyaniseka njengesiseko, Uncedo olunyanisekileyo kunye nenzuzo efanayo" ngumbono wethu, njengendlela yokuvelisa ngokuqhubekayo kunye nokulandela ukugqwesa kwexabiso leWholesale yaseChina Iindleko eziSebenzayo eziSebenzayo kwiMakethe ye-DNA kwi-Realplace Manufacturer I-Rednassos ukutya okukhawulezayo kunye neziselo ezityiwayo kulo lonke iplanethi, Sifuna phambili ekusebenzeni namaqabane / abathengi ukwenza iziphumo ezilungileyo kunye.
Ixabiso leWholesaleChina Universal Rna DNA Reagents, Ukuhlanjululwa kwe-Acid ye-Nucleic ngokuzenzekelayo, Inkampani yethu ngoku inamasebe amaninzi, kwaye kukho abasebenzi abangaphezu kwama-20 kwinkampani yethu.Siye saseka ivenkile yokuthengisa, igumbi lokubonisa, kunye nendawo yokugcina imveliso.Okwangoku, sibhalise igama lethu.Siye saqinisa uhlolo lomgangatho wemveliso.